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c-Jun (JUN) (NM_002228) Human Untagged Clone

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SKU
SC118762
JUN (untagged)-Human jun proto-oncogene (JUN)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol c-Jun
Synonyms AP-1; AP1; c-Jun; cJUN; p39
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_002228 edited
GAATTCGGCACGAGGGTGGCTGAAGCAGCGAGGCGGGAGTGGAGGTGCGCGGAGTCAGGC
AGACAGACAGACACAGCCAGCCAGCCAGGTCGGCAGTATAGTCCGAACTGCAAATCTTAT
TTTCTTTTCACCTTCTCTCTAACTGCCCAGAGCTAGCGCCTGTGGCTCCCGGGCTGGTGT
TTCGGGAGTGTCCAGAGAGCCTGGTCTCCAGCCGCCCCCGGGAGGAGAGCCCTGCTGCCC
AGGCGCTGTTGACAGCGGCGGAAAGCAGCGGTACCCACGCGCCCGCCGGGGGAAGTCGGC
GAGCGGCTGCAGCAGCAAAGAACTTTCCCGGCTGGGAGGACCGGAGACAAGTGGCAGAGT
CCCGGAGCGAACTTTTGCAAGCCTTTCCTGCGTCTTAGGCTTCTCCACGGCGGTAAAGAC
CAGAAGGCGGCGGAGAGCCACGCAAGAGAAGAAGGACGTGCGCTCAGCTTCGCTCGCACC
GGTTGTTGAACTTGGGCGAGCGCGAGCCGCGGCTGCCGGGCGCCCCCTCCCCCTAGCAGC
GGAGGAGGGGACAAGTCGTCGGAGTCCGGGCGGCCAAGACCCGCCGCCGGCCGGCCACTG
CAGGGTCCGCACTGATCCGCTCCGCGGGGAGAGCCGCTGCTCTGGGAAGTGAGTTCGCCT
GCGGACTCCGAGGAACCGCTGCGCCCGAAGAGCGCTCAGTGAGTGACCGCGACTTTTCAA
AGCCGGGTAGCGCGCGCGAGTCGACAAGTAAGAGTGCGGGAGGCATCTTAATTAACCCTG
CGCTCCCTGGAGCGAGCTGGTGAGGAGGGCGCAGCGGGGACGACAGCCAGCGGGTGCGTG
CGCTCTTAGAGAAACTTTCCCTGTCAAAGGCTCCGGGGGGCGCGGGTGTCCCCCGCTTGC
CAGAGCCCTGTTGCGGCCCCGAAACTTGTGCGCGCAGCCCAAACTAACCTCACGTGAAGT
GACGGACTGTTCTATGACTGCAAAGATGGAAACGACCTTCTATGACGATGCCCTCAACGC
CTCGTTCCTCCCGTCCGAGAGCGGACCTTATGGCTACAGTAACCCCAAGATCCTGAAACA
GAGCATGACCCTGAACCTGGCCGACCCAGTGGGGAGCCTGAAGCCGCACCTCCGCGCCAA
GAACTCGGACCTCCTCACCTCGCCCGACGTGGGGCTGCTCAAGCTGGCGTCGCCCGAGCT
GGAGCGCCTGATAATCCAGTCCAGCAACGGGCACATCACCACCACGCCGACCCCCACCCA
GTTCCTGTGCCCCAAGAACGTGACAGATGAGCAGGAGGGCTTCGCCGAGGGCTTCGTGCG
CGCCCTGGCCGAACTGCACAGCCAGAACACGCTGCCCAGCGTCACGTCGGCGGCGCAGCC
GGTCAACGGGGCAGGCATGGTGGCTCCCGCGGTAGCCTCGGTGGCAGGGGGCAGCGGCAG
CGGCGGCTTCAGCGCCAGCCTGCACAGCGAGCCGCCGGTCTACGCAAACCTCAGCAACTT
CAACCCAGGCGCGCTGAGCAGCGGCGGCGGGGCGCCCTCCTACGGCGCGGCCGGCCTGGC
CTTTCCCGCGCAACCCCAGCAGCAGCAGCAGCCGCCGCACCACCTGCCCCAGCAGATGCC
CGTGCAGCACCCGCGGCTGCAGGCCCTGAAGGAGGAGCCTCAGACAGTGCCCGAGATGCC
CGGCGAGACACCGCCCCTGTCCCCCATCGACATGGAGTCCCAGGAGCGGATCAAGGCGGA
GAGGAAGCGCATGAGGAACCGCATCGCTGCCTCCAAGTGCCGAAAAAGGAAGCTGGAGAG
AATCGCCCGGCTGGAGGAAAAAGTGAAAACCTTGAAAGCTCAGAACTCGGAGCTGGCGTC
CACGGCCAACATGCTCAGGGAACAGGTGGCACAGCTTAAACAGAAAGTCATGAACCACGT
TAACAGTGGGTGCCAACTCATGCTAACGCAGCAGTTGCAAACATTTTGAAGAGAGACCGT
CGGGGGCTGAGGGGCAACGAAAAAAAAAAAAAAAAAACTCGAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002228 unedited
GAATTCGGCACGAGGGTGGCTGAAGCAGCGAGGCGGGAGTGGAGGTGCGCGGAGTCAGGC
AGACAGAACAGAACACAGCCAGCCAGCCAGGTCGGCAGTATAGTCCGAACTGCAAATCTT
ATTTTCTTTTCACCTTCTCTCTAACTGCCCAGAAGCTAGCGCCTGTGGCTCCCGGGCTGG
TGTTTCGGGAGTGTCCAGAGAGCCTGGTCTCCAGCCGCCCCCGGGAGGAGAGCCCTGCTG
CCCAGGCGCTGTTGACAGCGGCGGAAAGCAGCGGTACCCACGCGCCCGCCGGGGGAAGTC
GGCGAGCGGCTGCAGCAGCAAAGAACTTTCCCGGCTGGGAGGACCGGAGACAAGTGGCAG
AGTCCCGGAGCGAACTTTTGCAAGCCTTTCCTGCGTCTTAGGCTTCTCCACGGCGGTAAA
GACCAGAAGGCGGCGGAGAGCCACGCAAGAGAAGAAGGACGTGCGCTCAGCTTCGCTCGC
ACCGGTTGTTGAACTTGGGCGAGCGCGAGCCGCGGCTGCCGGGCGCCCCCTCCCCCTAGC
AGCGGAGGAGGGGACAAGTCGTCGGAGTCCGGGCGGCCAGACCCNNGCGCGGCCGGCACT
GCANGGGTCGCACTGATCCGCTCCGCGGGGAGAGCCGCTGCTTGGGAAGTGAGGTCGCCT
GCGGGACTCGAGAACCGCTGCGCCGAGAGCGCTCAGTGAGTGACCGCGACTTTTCAAGCC
GGNTANCGCGCGCGAGTCCACAGTTAAAAGTGCGGGAGGCATCTTATTTAACCCTGCGCT
CCCTGA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002228 unedited
TTAGCTATGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCGTTGC
CCCTCAGCCCCCGACGGTCTCTCTTCAAAATGTTTGCAACTGCTGCGTTAGCATGAGTTG
GCACCCACTGTTAACGTGGTTCATGACTTTCTGTTTAAGCTGTGCCACCTGTTCCCTGAG
CATGTTGGCCGTGGACGCCAGCTCCGAGTTCTGAGCTTTCAAGGTTTTCACTTTTTCCTC
CAGCCGGGCGATTCTCTCCAGCTTCCTTTTTCGGCACTTGGAGGCAGCGATGCGGTTCCT
CATGCGCTTCCTCTCCGCCTTGATCCGCTCCTGGGACTCCATGTCGATGGGGGACAGGGG
CGGTGTCTCGCCGGGCATCTCGGGCACTGTCTGAGGCTCCTCCTTCAGGGCCTGCAGCCG
CGGGTGCTGCACGGGCATCTGCTGGGGCAGGTGGTGCGGCGGCTGCTGCTGCTGCTGGGG
TTGCGCGGGAAAGGCCAGGCCGGCCGCGCCGTAGGAGGGCGCCCCGCCGCCGCTGCTCAN
CGCGCCTGGGTTGAAGTTGCTGAGGTTTGCGTAAACCGGCGGCTCGCTGTGCAGGCTGGC
GCTGAANCCGCCGCTGCCGCTGCCCCCCTGCCACCGAGGCTACCGCGGGAGCCACCATGC
CTGCCCCGTTGACCGGCTGCGCCGCCGACGTGACGCTGGGCAGCGTGTTCTGGCTGTGCC
AATTCGCCAGGGCGCGCACGAAACCCTTCGCGAAGCCCTCCTGCTCATCTGTCACGTTCT
TGGGGCACAGGAACTGGGGTGGGGGTCGCGGTGGGGGGGAATTACCCCCTTCTTGGACTG
GATTATAAGGGCCTTCACTTGGGGGGAGGCCAGCTTGAGAATCCCCACCTCGGGGAGGTG
GGGGGGGCTCATTTCTTGGTCCCGGGGGGCGGGTTCAAGCTCAC
Restriction Sites NotI-NotI |
ACCN NM_002228
Insert Size 2080 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002228.3, NP_002219.1
RefSeq Size 3338 bp
RefSeq ORF 996 bp
Locus ID 3725
UniProt ID P05412
Cytogenetics 1p32.1
Domains BRLZ, Jun
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
Protein Pathways B cell receptor signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, Pathways in cancer, Renal cell carcinoma, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Wnt signaling pathway
Summary This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_002228" in other vectors (6)
SKU Description Size Price
RC209804 JUN (Myc-DDK-tagged)-Human jun proto-oncogene (JUN) 10 ug
$300.00
RC209804L1 Lenti ORF clone of Human jun proto-oncogene (JUN), Myc-DDK-tagged 10 ug
$600.00
RC209804L2 Lenti ORF clone of Human jun proto-oncogene (JUN), mGFP tagged 10 ug
$600.00
RC209804L3 Lenti ORF clone of Human jun proto-oncogene (JUN), Myc-DDK-tagged 10 ug
$600.00
RC209804L4 Lenti ORF clone of Human jun proto-oncogene (JUN), mGFP tagged 10 ug
$600.00
RG209804 JUN (tGFP-tagged) - Human jun proto-oncogene (JUN) 10 ug
$489.00 $500.00

file-alt Citations

FOS and JUN regulate oxidative stress and steroidogenesis in human aldosterone-producing adenomas
Wei, Duregon, Papotti et al
Redox Biol (2025) 89, 103982 DOI: 10.1016/j.redox.2025.103982
Cited by 3
AI Summary: In this study, OriGene's JUN cDNA (SC118762) was used to co-overexpress JUN in HAC15 cells, enabling the authors to investigate the role of JUN in regulating oxidative stress and steroidogenesis in aldosterone-producing adenomas.
Snippet: One million HAC15 cells were co-transfected with FOS cDNA (GenScript, OHu22292) and JUN cDNA (Origene, SC118762) using the Cell Line Nucleofector Kit R (Amaxa, VCA-1001) according to the manufacturer's instruction. After 48 h of transfection, cells were harvested for PCR and Western blotting analysis
Suppression of endothelial nitric oxide synthase expression and endothelial cell proliferation by an intronic 27-ntmiRNA and it's a novel link to AP-1
Li, Yan, Zhang et al
Am J Transl Res (2015) 7 (2), 285-97
AI Summary: In this study, the pCMV6-XL4-AP-1 plasmid from OriGene Technologies was used to overexpress AP-1 protein in endothelial cells, enabling the researchers to demonstrate that AP-1 can partially restore eNOS expression and cell proliferation suppressed by 27nt-miRNA.
Snippet: Anti-AP-1 antibody was from Santa Cruz (Santa Cruz, CA, USA). Methylthiazolyl blue tetrazolium (MTT) and dimethyl sulphoxide (DMSO) were from Sigma (St. Louis, MO, USA). The pCMV6-XL4-AP-1 plasmid was from OriGene Technologies, Inc. (Rockville, MD, USA ... Cite SaveSaving
The nuclear import of oncoprotein hepatitis B X-interacting protein depends on interacting with c-Fos and phosphorylation of both proteins in breast cancer cells
Zhang, Zhao, Li et al
J. Biol. Chem. (2013) 288 (26), 18961-74 DOI: 10.1074/jbc.M113.458638
AI Summary: OriGene products, specifically the pCMV-c-Fos and pCMV-c-Jun plasmids, were used in this study to investigate the interactions and nuclear import mechanisms of HBXIP and c-Fos in breast cancer cells.
Snippet: The full-length pCMV-c-Fos and pCMV-c-Jun plasmids were purchased from OriGene Technologies Company (USA). ... The value is obtained by the intensity ratio between the target protein and β-actin band for cytoplasmic fractionation in each lane.
Complement-mediated regulation of the IL-17A axis is a central genetic determinant of the severity of experimental allergic asthma
Lajoie, Lewkowich, Suzuki et al
Nat Immunol (2010) 11 (10), 928-35 DOI: 10.1038/ni.1926
Cited by 330
AI Summary: OriGene products contributed to this study by providing specific qPCR primers (Mm-Il17a-01, Mm-Il23a-01, and Mm-Il17ra-01) used to quantify gene expression levels of IL-17A, IL-23a, and IL-17 receptor A, enabling precise analysis of cytokine regulation in experimental allergic asthma.
FigureFigureFigure
Surfactant-associated protein B is critical to survival in nickel-induced injury in mice
Bein, Wesselkamper, Liu et al
Am J Respir Cell Mol Biol (2009) 41 (2), 226-36 DOI: 10.1165/rcmb.2008-0317OC
AI Summary: OriGene products contributed to this study by providing the SFTPB cDNA clone (OriGene, MC202874) and SFTPB-specific qPCR primers (OriGene, MP208447), which were used to analyze and manipulate SFTPB expression levels in mouse lung epithelial cells and in transgenic mice.
Surfactant-associated protein B is critical to survival in nickel-induced injury in mice
Bein, Wesselkamper, Liu et al
Am J Respir Cell Mol Biol (2009) 41 (2), 226-36 DOI: 10.1165/rcmb.2008-0317OC
AI Summary: OriGene products contributed to this study by providing the mouse SFTPB cDNA (OriGene, MC200446) used for transfection experiments to assess the role of surfactant-associated protein B in nickel-induced lung injury.
Showing 1-6 of 7 papers.
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