WEE1 (NM_003390) Human Tagged ORF Clone
$601.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | WEE1 |
| Synonyms | WEE1A; WEE1hu |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003390 |
| ORF Size | 1938 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003390.4 |
| RefSeq Size | 4232 bp |
| RefSeq ORF | 1941 bp |
| Locus ID | 7465 |
| UniProt ID | P30291 |
| Cytogenetics | 11p15.4 |
| Domains | pkinase, S_TKc, TyrKc |
| Protein Families | Druggable Genome, Protein Kinase, Stem cell - Pluripotency |
| Protein Pathways | Cell cycle |
| MW | 71.4 kDa |
| Summary | This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003390" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209760L1 | Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$901.00
|
|
| RC209760L2 | Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1, mGFP tagged | 10 ug |
$901.00
|
|
| RC209760L3 | Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$901.00
|
|
| RC209760L4 | Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1, mGFP tagged | 10 ug |
$901.00
|
|
| RG209760 | WEE1 (tGFP-tagged) - Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1 | 10 ug |
$489.00
$801.00
|
|
| SC117999 | WEE1 (untagged)-Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1 | 10 ug |
$602.00
|
|
| SC323510 | WEE1 (untagged)-Kinase deficient mutant (K328M) of Human WEE1 homolog (S. pombe) (WEE1) | 10 ug |
$602.00
|
|
Citations
YTHDF2 promotes mitotic entry and is regulated by cell cycle mediators
Fei, Zou, Roundtree et al
PLoS Biol. (2020) 18 (4), e3000664 DOI: 10.1371/journal.pbio.3000664
Fei, Zou, Roundtree et al
PLoS Biol. (2020) 18 (4), e3000664 DOI: 10.1371/journal.pbio.3000664
Cited by 112
AI Summary: OriGene products, specifically the WEE1 construct (Cat# RC209760) and the empty vector control (Cat# PS100001), were used in this study for plasmid transfection experiments to investigate the role of YTHDF2 in regulating cell cycle progression.
Snippet: … The WEE1 construct (Category Number RC209760) and empty vector control (Category Number PS100001) were ordered from OriGene. …The WEE1 construct (Category Number RC209760) and empty vector control (Category Number PS100001 [https://www.origene.com/catalog/vectors/tagged-cloning-vectors/ps100001/pcmv6-entry-tagged-cloning-vector]) were ordered from OriGene. …
Circadian rhythm disruption impairs tissue homeostasis and exacerbates chronic inflammation in the intestine
Pagel, Bär, Schröder et al
FASEB J. (2017)
Pagel, Bär, Schröder et al
FASEB J. (2017)
AI Summary: In this study, OriGene products pCMV6-Entry (PS100001) and pCMV6-Entry-Wee1 vector (RC209760) were used for overexpression experiments to investigate the role of Wee1 in intestinal epithelial cell apoptosis and cell cycle regulation under circadian rhythm disruption.
Snippet: … PS100001;OriGene Technologies, Rockville, MD, USA) or pCMV6-Entry-Wee1 vector (RC209760;OriGene Technologies) by lipofection with Lipofectamine 2000, according to the manufacturer's instruc- tions. …PS100001;OriGene Technologies) by lipofection with Lipofectamine 2000, according to the manufacturer's instruc- tions. …
Showing 1-2 of 2 papers.
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