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WEE1 (NM_003390) Human Untagged Clone

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SKU
SC323510
WEE1 (untagged)-Kinase deficient mutant (K328M) of Human WEE1 homolog (S. pombe) (WEE1)
  $602.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol WEE1
Synonyms WEE1A; WEE1hu
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC323510 sequence for NM_003390 edited (data generated by NextGen Sequencing)
ATGAGCTTCCTGAGCCGACAGCAGCCGCCGCCACCCCGCCGCGCCGGGGCGGCCTGCACC
TTGCGGCAGAAGCTGATCTTCTCGCCCTGCAGCGACTGTGAGGAGGAGGAAGAAGAGGAG
GAGGAGGAGGGCAGCGGCCACAGCACCGGGGAGGACTCGGCCTTTCAAGAGCCCGACTCG
CCGCTGCCGCCCGCGCGGAGCCCCACGGAGCCCGGGCCCGAGCGCCGCCGCTCGCCCGGG
CCGGCCCCCGGGAGCCCCGGCGAGCTGGAGGAGGACCTGTTGCTGCCCGGCGCCTGCCCG
GGCGCGGACGAGGCGGGCGGTGGGGCGGAGGGCGACTCGTGGGAGGAGGAGGGCTTCGGC
TCCTCGTCGCCGGTCAAGTCGCCGGCGGCCCCCTACTTCCTGGGTAGCTCTTTCTCGCCG
GTGCGCTGCGGCGGCCCAGGAGATGCGTCGCCGCGGGGTTGCGGGGCGCGCCGGGCGGGC
GAAGGCCGCCGCTCGCCGCGGCCGGACCACCCGGGCACCCCGCCACACAAGACCTTCCGC
AAGCTGCGACTCTTCGACACCCCGCACACGCCCAAGAGTTTGCTCTCCAAAGCTCGGGGA
ATTGATTCCAGCTCTGTTAAACTCCGGGGTAGTTCTCTCTTCATGGATACAGAAAAATCA
GGAAAAAGGGAATTTGATGTGCGACAGACTCCTCAAGTGAATATTAATCCTTTTACTCCG
GATTCTTTGTTGCTTCATTCCTCAGGACAGTGTCGTCGTAGAAAGAGAACGTATTGGAAT
GATTCCTGTGGTGAAGACATGGAAGCCAGTGATTATGAGCTTGAAGATGAAACAAGACCT
GCTAAGAGAATTACAATTACTGAAAGCAATATGAAGTCCCGGTATACAACAGAATTTCAT
GAGCTAGAGAAAATCGGCTCTGGAGAATTTGGTTCTGTATTTAAGTGTGTGAAGAGGCTG
GATGGATGCATTTATGCCATTATGCGATCAAAAAAGCCATTGGCGGGCTCTGTTGATGAG
CAGAACGCTTTGAGAGAAGTATATGCTCATGCAGTGCTTGGACAGCATTCTCATGTAGTT
CGATATTTCTCTGCGTGGGCAGAAGATGATCATATGCTTATACAGAATGAATATTGTAAT
GGTGGAAGTTTAGCTGATGCTATAAGTGAAAACTACAGAATCATGAGTTACTTTAAAGAA
GCAGAGTTGAAGGATCTCCTTTTGCAAGTTGGCCGAGGCTTGAGGTATATTCATTCAATG
TCTTTGGTTCACATGGATATAAAACCTAGTAATATTTTCATATCTCGAACCTCAATCCCA
AATGCTGCCTCTGAAGAAGGAGACGAAGATGATTGGGCATCCAACAAAGTTATGTTTAAA
ATAGGTGATCTTGGGCATGTAACAAGGATCTCCAGTCCACAAGTTGAAGAGGGCGATAGT
CGTTTTCTTGCAAATGAAGTTTTACAGGAGAATTATACCCATCTACCAAAAGCAGATATT
TTTGCGCTTGCCCTCACAGTGGTATGTGCTGCTGGTGCTGAACCTCTTCCGAGAAATGGA
GATCAATGGCATGAAATCAGACAGGGTAGATTACCTCGGATACCACAAGTGCTTTCCCAA
GAATTTACAGAGTTGCTAAAAGTTATGATTCATCCAGATCCAGAGAGAAGACCTTCAGCA
ATGGCACTGGTAAAGCATTCAGTATTGCTGTCCGCTTCTAGAAAGAGTGCAGAACAATTA
CGAATAGAATTGAATGCCGAAAAGTTCAAAAATTCACTTTTACAAAAAGAACTCAAGAAA
GCACAGATGGCAAAAGCTGCAGCTGAGGAAAGAGCACTCTTCACTGACCGGATGGCCACT
AGGTCCACCACCCAGAGTAATAGAACATCTCGACTTATTGGAAAGAAAATGAACCGCTCT
GTCAGCCTTACTATATACTGA

Clone variation with respect to NM_003390.3
252 c=>g;983 a=>t
5' Read Nucleotide Sequence
>OriGene 5' read for mutant NM_003390 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGCCCCGCCTCT
GCCGGAAAGTCCGCGCCGCCGCTGCCGCCACCGTCCGCAGCCCGAGCGCCCCGGAGCCGCAGGCCGCCGC
CGCGCAGAGACGCCGCGGCTGCGACTAGGCGCGCCCAGCCGCACGTGGCGGACCCGCCCCCAGGCCCCGC
AGTGTCCTGGACCCCGCAGGCCTCCGCTCTCCTGTCCCTCGGCCCCGTCCCCAGGGCCCGCGATGAGCTT
TCCTGAGCCCGACAGCAGCCCGCCGCCCACCCCGCCCGCGCCGGGGGCGGCCTGCACCTTGGCGGCGGAG
CCTGATTCTTCCTCGCCCCTGCAGCCGACTGGTGAGGAGGAGGAAGAAGAGGAGGAAGGAGGAGGGCACC
GGCCACAGACCGGGAAGGCTTCGGCCTTCAGAACCCAACTGCCGTTGCGCCGGCCGAGCCCCGGACCCGG
GCCAAGCCCGCGTTCGCCGGCCGGCCCGGGGACCCGGAACTGGAGAGACGTGTGTGCG
Kinase Domain Sequence
>SC323510 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCGGCTWACACAGATTTCATGAGCTAGAGAAAATCGGCTCTGGAGAATTTGGTTCTGTATTTAAGTGTGT
GAAGAGGCTGGATGGATGCATTTATGCCATTATGCGATCAAAAAAGCCATTGGCGGGCTCTGTTGATGAG
CAGAACGCTTTGAGAGAAGTATATGCTCATGCAGTGCTTGGACAGCATTCTCATGTAGTTCGATATTTCT
CTGCGTGGGCAGAAGATGATCATATGCTTATACAGAATGAATATT
Restriction Sites Please inquire |
ACCN NM_003390
Insert Size 3670 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003390.2, NP_003381.1
RefSeq Size 4232 bp
RefSeq ORF 1941 bp
Locus ID 7465
UniProt ID P30291
Cytogenetics 11p15.4
Domains pkinase, S_TKc, TyrKc
Protein Families Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways Cell cycle
Summary This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_003390" in other vectors (7)
SKU Description Size Price
RC209760 WEE1 (Myc-DDK-tagged)-Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1 10 ug
$601.00
RC209760L1 Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1, Myc-DDK-tagged 10 ug
$901.00
RC209760L2 Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1, mGFP tagged 10 ug
$901.00
RC209760L3 Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1, Myc-DDK-tagged 10 ug
$901.00
RC209760L4 Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1, mGFP tagged 10 ug
$901.00
RG209760 WEE1 (tGFP-tagged) - Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1 10 ug
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SC117999 WEE1 (untagged)-Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1 10 ug
$602.00

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