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MLC1 (NM_015166) Human Tagged ORF Clone

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SKU
RC209731
MLC1 (Myc-DDK-tagged)-Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MLC1
Synonyms LVM; MLC; VL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209731 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCAGGAGCCATTCAGAGAGGAGCTAGCCTATGACCGGATGCCCACGCTGGAGCGGGGCCGGCAAG
ACCCCGCCAGCTATGCCCCAGACGCGAAGCCGAGCGACCTGCAGCTGTCGAAGAGACTGCCCCCCTGCTT
CAGCCACAAGACGTGGGTCTTCTCTGTGCTGATGGGGAGCTGCCTCCTGGTGACCTCGGGGTTTTCGCTG
TACCTGGGGAACGTGTTCCCGGCTGAGATGGATTACTTGCGCTGTGCTGCAGGCTCTTGCATCCCCTCGG
CAATTGTGAGCTTCACCGTCTCCAGGAGGAACGCCAATGTGATTCCCAACTTTCAGATATTGTTTGTTTC
CACGTTTGCTGTGACCACTACGTGTTTAATTTGGTTTGGATGCAAACTAGTCCTGAACCCATCAGCAATA
AACATCAACTTCAACCTCATCCTGCTGCTCCTGCTGGAGCTGCTCATGGCGGCCACGGTGATCATCGCTG
CACGGTCCAGCGAGGAGGACTGCAAGAAAAAGAAGGGCTCCATGTCTGACAGCGCCAACATTCTGGACGA
AGTGCCATTTCCTGCTCGGGTCCTGAAATCTTACTCAGTCGTCGAGGTAATCGCAGGCATCTCTGCCGTC
CTCGGGGGGATCATTGCCCTGAACGTGGATGACTCAGTTTCAGGCCCACACCTCTCAGTGACGTTCTTTT
GGATCCTAGTGGCCTGCTTTCCAAGTGCCATTGCCAGTCATGTGGCAGCAGAGTGTCACAGCAAGTGTCT
GGTGGAGGTCCTGATTGCCATAAGCAGCCTCACGTCTCCGCTGCTGTTCACAGCCTCTGGATATCTGTCA
TTCAGCATCATGAGAATCGTGGAGATGTTTAAGGATTACCCGCCAGCCATAAAACCATCCTACGATGTGC
TGCTGCTGCTGCTGCTGCTAGTGCTCCTGCTGCAGGCCGGCCTCAACACGGGCACCGCCATCCAGTGCGT
GCGCTTCAAGGTCAGTGCAAGGCTGCAGGGTGCATCCTGGGACACCCAGAACGGCCCGCAGGAGCGCCTG
GCTGGGGAGGTGGCCAGGAGCCCCCTGAAGGAGTTCGACAAGGAGAAAGCCTGGAGAGCCGTCGTGGTGC
AAATGGCCCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209731 protein sequence
Red=Cloning site Green=Tags(s)

MTQEPFREELAYDRMPTLERGRQDPASYAPDAKPSDLQLSKRLPPCFSHKTWVFSVLMGSCLLVTSGFSL
YLGNVFPAEMDYLRCAAGSCIPSAIVSFTVSRRNANVIPNFQILFVSTFAVTTTCLIWFGCKLVLNPSAI
NINFNLILLLLLELLMAATVIIAARSSEEDCKKKKGSMSDSANILDEVPFPARVLKSYSVVEVIAGISAV
LGGIIALNVDDSVSGPHLSVTFFWILVACFPSAIASHVAAECHSKCLVEVLIAISSLTSPLLFTASGYLS
FSIMRIVEMFKDYPPAIKPSYDVLLLLLLLVLLLQAGLNTGTAIQCVRFKVSARLQGASWDTQNGPQERL
AGEVARSPLKEFDKEKAWRAVVVQMAQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015166
ORF Size 1131 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015166.4
RefSeq Size 3960 bp
RefSeq ORF 1134 bp
Locus ID 23209
UniProt ID Q15049, A0A024R4V4
Cytogenetics 22q13.33
Protein Families Ion Channels: Other, Transmembrane
MW 41.2 kDa
Summary The function of this gene product is unknown; however, homology to other proteins suggests that it may be an integral membrane transporter. Mutations in this gene have been associated with megalencephalic leukoencephalopathy with subcortical cysts, an autosomal recessive neurological disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Jul 2008
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"NM_015166" in other vectors (4)
SKU Description Size Price
RC209731L3 Lenti ORF clone of Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC209731L4 Lenti ORF clone of Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG209731 MLC1 (tGFP-tagged) - Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1 10 ug
$657.00
SC102570 MLC1 (untagged)-Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1 10 ug
$457.00

Plasmid Map for RC209731

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209731
Circular map for RC209731

file-alt Citations

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