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MLC1 (NM_015166) Human Untagged Clone

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SKU
SC102570
MLC1 (untagged)-Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MLC1
Synonyms LVM; MLC; VL
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC102570 sequence for NM_015166 edited (data generated by NextGen Sequencing)
ATGACCCAGGAGCCATTCAGAGAGGAGCTGGCCTATGACCGGATGCCCACGCTGGAGCGG
GGCCGGCAAGACCCCGCCAGCTATGCCCCAGACGCGAAGCCGAGCGACCTGCAGCTGTCG
AAGAGACTGCCCCCCTGCTTCAGCCACAAGACGTGGGTCTTCTCTGTGCTGATGGGGAGC
TGCCTCCTGGTGACCTCGGGGTTTTCGCTGTACCTGGGGAACGTGTTCCCGGCTGAGATG
GATTACTTGCGCTGTGCTGCAGGCTCTTGCATCCCCTCGGCAATTGTGAGCTTCACCGTC
TCCAGGAGGAACGCCAATGTGATTCCCAACTTTCAGATATTGTTTGTTTCCACGTTTGCT
GTGACCACTACGTGTTTAATTTGGTTTGGATGCAAACTAGTCCTGAACCCATCAGCAATA
AACATCAACTTCAACCTCATCCTGCTGCTCCTGCTGGAGCTGCTCATGGCGGCCACGGTG
ATCATCGCTGCACGGTCCAGCGAGGAGGACTGCAAGAAAAAGAAGGGCTCCATGTCTGAC
AGCGCCAACATTCTGGACGAAGTGCCATTTCCTGCTCGGGTCCTGAAATCTTACTCAGTC
GTCGAGGTAATCGCAGGCATCTCTGCCGTCCTCGGGGGGATCATTGCCCTGAACGTGGAT
GACTCAGTTTCAGGCCCACACCTCTCAGTGACGTTCTTTTGGATCCTAGTGGCCTGCTTT
CCAAGTGCCATTGCCAGTCATGTGGCAGCAGAGTGTCCCAGCAAGTGTCTGGTGGAGGTC
CTGATTGCCATAAGCAGCCTCACGTCTCCGCTGCTGTTCACAGCCTCTGGATATCTGTCA
TTCAGCATCATGAGAATCGTGGAGATGTTTAAGGATTACCCGCCAGCCATAAAACCATCC
TACGATGTGCTGCTGCTGCTGCTGCTGCTAGTGCTCCTGCTGCAGGCCGGCCTCAACACG
GGCACCGCCATCCAGTGCGTGCGCTTCAAGGTCAGTGCAAGGCTGCAGGGTGCATCCTGG
GACACCCAGAACGGCCCGCAGGAGCGCCTGGCTGGGGAGGTGGCCAGGAGCCCCCTGAAG
GAGTTCGACAAGGAGAAAGCCTGGAGAGCCGTCGTGGTGCAAATGGCCCAGTGA

Clone variation with respect to NM_015166.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_015166 unedited
ATTTTGTAATACGACTTACTATAGGGCGGCCCGCGATTCGGCACGAGGCGTTGAGTAGAG
GCATGTTTTGCAATCTCTCGTTTAGCTACCAATTGGAGCAGTTTAACGTGCTTGAGTCCC
CGGCGTGATGTGTTCATGGACGAAGTTGCCGATGGATGTTGTCAGGTGTTGCACCTGGAG
GGCCCAGATGGATCAATGAGGCATTTAAAGTGTAGAATCTCACCCTGAACCCAGACTATT
CAGGTCCGTTTTTCTAGGAAAGTTATTTCTTTTTCACTTTTAGATTCAAGTGCTCATTTT
CTTCTTCAAATTTAGAGCACTTCAGTATGAACAATATTTCTTTTGTTAAAAAATATTTCT
TGGAAACAACTTATTGAGGTTTCCTCAGAGTGGCCAAAGCCGATGATAATTTTATTCCAG
ATAAAGAACTGGTGACACGTGGCTGTACATTCAGCACAGCTGTGGTGTCCCCAAGTGCCA
TGACCCAGGAGCCATTCAGAGAGGAGCTGGCCTATGACCGGATGCCCACGCTGGAGCGGG
GCCGGCAAGACCCCGCCAGCTATGCCCCAGACGCGAAGCCGAGCGACCTGCAGCTGTCGA
AGAGACTGCCCCCCTGCTTCAGCCACAAGACGTGGGTCTTCTCTGTGCTGATGGGGAGCT
GCCTCCTGGTGACCTCGGGGTTTTCGCTGTACCTGCGGAACGTGTTCCCGGCTGAGATGG
ATTACTTGCGCTGTGCTGCAGGCTCTTGCATCCCCTTCGCCATTGTGAGCTTCACCGTCT
CCAGNAGGAACGCCAATGTGATTCCCAACTTTCAGATATTGTTGTTTCCACGCTTGCTGT
GACCACTACGTGTTTAATTTGGGTTTGGATGCAACTAGTTCTGAACCCATTAGCATTAAC
CATTACTTCACCTCATCCTGCTGCTCCTGCTGGAGCTGCTCATTGGGGCCCAGTGATCAT
TCGTGACGTTCCACGCAGAAGACTGCATA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_015166 unedited
GGGTTTAGCTATGNANCGCGGCCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTGC
AGTCAGCGTTTATGCTCAGCGTTTAAACAAAAAGTAGACTGAAAGAGAAGTTCTTTTAAA
CCCCATTTTTCACGATTTTTTAAGCGCTCTAAAATAAGAAAATAAGAAAGTGCAAGCCAG
CAAAAACGCTCCAAGTGCCTAATTCTGACTCTGAAACTTGAGCTCTCTGGTCTGCCCCCA
AGAAGACATCAGCCCGCCCCGGGTCGTCCCTGTGGCTCCCACCCCATTCCCAGGAGCAGA
CCCCGCCAGCCTCAAAGCTGCAGGGAGGTGGGGGTGGCCTGCAGACAGGGTGGGGTCTGC
ATCCGGTACCAGTGACAGCAGCCTCTCCTCTCCCACGGTGGCGCTTGTTTGGGGCTGTGG
CCAAAGTGTTTGCCCGGCCCCTGACTGTGTCCTTCCGGAGCTGCCGAGGACTGAAGAGAG
GGCCTGGCTTGTCCCCTCTAGGAGCAGCTGGGAAGGTGTCTTGCCTGCATCCCCCTTCAA
TGGTTGAAAATAATGATTCCACTTGTCATGAACACCATGAAGGTATCTTGGCAGCCAGAG
TCACTCCTGTTCCGCAGTGGGAAACCTGGGAGGGTCCTCAAACCCCCTGGCAGGGTCTGC
AGGCCCGCCCCATTCAGCTGCATCTCCCAAGCCTCCTGGTTCTTTTGATCTTGATGGCCC
CAAGCCACAGATGCATCTTCGGGCCTTTCCAGCAGCCCATGGGGGACCAGCCAATACACC
CCCACGGGGGTGGAAGAGAAAACGTTAATGTCTTTCTCAGATCAGAAGGAAAGAACCAAA
CCCTTGCTGAAGAAAACACTGCTGGAAGTAATTATAAAGTAACAGCATTCCAGTTCC
Restriction Sites NotI-NotI |
ACCN NM_015166
Insert Size 4230 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015166.2, NP_055981.1
RefSeq Size 3516 bp
RefSeq ORF 1134 bp
Locus ID 23209
UniProt ID Q15049
Cytogenetics 22q13.33
Protein Families Ion Channels: Other, Transmembrane
Summary The function of this gene product is unknown; however, homology to other proteins suggests that it may be an integral membrane transporter. Mutations in this gene have been associated with megalencephalic leukoencephalopathy with subcortical cysts, an autosomal recessive neurological disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.
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Documents
Resources
"NM_015166" in other vectors (4)
SKU Description Size Price
RC209731 MLC1 (Myc-DDK-tagged)-Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1 10 ug
$457.00
RC209731L3 Lenti ORF clone of Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC209731L4 Lenti ORF clone of Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG209731 MLC1 (tGFP-tagged) - Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1 10 ug
$657.00

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