STAT5B (NM_012448) Human Tagged ORF Clone
SKU
RC209429
STAT5B (Myc-DDK-tagged)-Human signal transducer and activator of transcription 5B (STAT5B)
$1,081.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | STAT5B |
| Synonyms | GHISID2; STAT5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012448 |
| ORF Size | 2361 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012448.4 |
| RefSeq Size | 5171 bp |
| RefSeq ORF | 2364 bp |
| Locus ID | 6777 |
| UniProt ID | P51692 |
| Cytogenetics | 17q21.2 |
| Domains | SH2, STAT |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors |
| Protein Pathways | Acute myeloid leukemia, Chemokine signaling pathway, Chronic myeloid leukemia, ErbB signaling pathway, Jak-STAT signaling pathway, Pathways in cancer |
| MW | 89.7 kDa |
| Summary | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012448" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209429L1 | Lenti ORF clone of Human signal transducer and activator of transcription 5B (STAT5B), Myc-DDK-tagged | 10 ug |
$1,381.00
|
|
| RC209429L2 | Lenti ORF clone of Human signal transducer and activator of transcription 5B (STAT5B), mGFP tagged | 10 ug |
$1,381.00
|
|
| RC209429L3 | Lenti ORF clone of Human signal transducer and activator of transcription 5B (STAT5B), Myc-DDK-tagged | 10 ug |
$1,381.00
|
|
| RC209429L4 | Lenti ORF clone of Human signal transducer and activator of transcription 5B (STAT5B), mGFP tagged | 10 ug |
$1,381.00
|
|
| RG209429 | STAT5B (tGFP-tagged) - Human signal transducer and activator of transcription 5B (STAT5B) | 10 ug |
$1,281.00
|
|
| SC115355 | STAT5B (untagged)-Human signal transducer and activator of transcription 5B (STAT5B) | 10 ug |
$1,082.00
|
|
Citations
Parallel genome-wide RNAi screens identify lymphocyte-specific protein tyrosine kinase (LCK) as a targetable vulnerability of cell proliferation and chemoresistance in nasopharyngeal carcinoma
Liew, Yu, Wei Pua et al
Cancer Lett (2021) 504, 81-90 DOI: 10.1016/j.canlet.2021.02.006
Liew, Yu, Wei Pua et al
Cancer Lett (2021) 504, 81-90 DOI: 10.1016/j.canlet.2021.02.006
Cited by 15
AI Summary: OriGene contributed to this study by providing the STAT5b expression construct, which was used to investigate the role of STAT5b in LCK-mediated proliferation and chemoresistance in nasopharyngeal carcinoma cells.
Snippet: The target sequences are listed in Supplemental Table 1. STAT5b expression construct was obtained from Origene, Rockville, MD, USA. Briefly
A novel heterozygous STAT5B variant in a patient with short stature and partial growth hormone insensitivity (GHI)
Ramírez, Sanguineti, Scaglia et al
Growth Horm IGF Res (2020) 50, 61-70 DOI: 10.1016/j.ghir.2019.12.005
Ramírez, Sanguineti, Scaglia et al
Growth Horm IGF Res (2020) 50, 61-70 DOI: 10.1016/j.ghir.2019.12.005
Cited by 11
A novel heterozygous STAT5B variant in a patient with short stature and partial growth hormone insensitivity (GHI)
Ramírez, Sanguineti, Scaglia et al
Growth Hormone & IGF Research (2020) 50, 61-70 DOI: 10.1016/j.ghir.2019.12.005
Ramírez, Sanguineti, Scaglia et al
Growth Hormone & IGF Research (2020) 50, 61-70 DOI: 10.1016/j.ghir.2019.12.005
Cited by 11
AI Summary: OriGene’s pCMV6-Entry STAT5B cDNA plasmid (RC209429) was used as the template for introducing the STAT5B gene variant, enabling functional analysis of the mutation described in the study.
Snippet: STAT5B gene variant was introduced into a commercial plasmid (pCMV6-Entry, RC209429, Origene, Rockville, MD, USA) containing the wild-type (WT) STAT5B cDNA (NM_012448), using the Quick Change II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa;- STAT5B gene variant was introduced into a commercial plasmid (pCMV6-Entry, RC209429, Origene, Rockville, MD, USA) containing the wild-type (WT) STAT5B cDNA (NM_012448), using the Quick Change II XL Site-Directed Mutagenesis Kit (Agilent Technologies
Type II enteropathy-associated T-cell lymphoma features a unique genomic profile with highly recurrent SETD2 alterations
Roberti, Dobay, Bisig et al
Nat Commun (2016) 7, 12602 DOI: 10.1038/ncomms12602
Roberti, Dobay, Bisig et al
Nat Commun (2016) 7, 12602 DOI: 10.1038/ncomms12602
Cited by 188
AI Summary: OriGene’s pCMV6-Entry STAT5B expression plasmid was used as a template to generate STAT5B mutants for functional studies in this paper.
Snippet: An expression plasmid pCMV6-Entry containing the wild-type sequencing of STAT5B (OriGene) was used as a template for site-directed mutagenesis to generate STAT5B mutants for functional study
Showing 1-4 of 4 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
