STAT5B (NM_012448) Human Tagged ORF Clone
- TrueORF®
SKU
RG209429
STAT5B (tGFP-tagged) - Human signal transducer and activator of transcription 5B (STAT5B)
$1,281.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | STAT5B |
| Synonyms | GHISID2; STAT5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012448 |
| ORF Size | 2361 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012448.4 |
| RefSeq Size | 5171 bp |
| RefSeq ORF | 2364 bp |
| Locus ID | 6777 |
| UniProt ID | P51692 |
| Cytogenetics | 17q21.2 |
| Domains | SH2, STAT |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors |
| Protein Pathways | Acute myeloid leukemia, Chemokine signaling pathway, Chronic myeloid leukemia, ErbB signaling pathway, Jak-STAT signaling pathway, Pathways in cancer |
| Summary | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012448" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209429 | STAT5B (Myc-DDK-tagged)-Human signal transducer and activator of transcription 5B (STAT5B) | 10 ug |
$1,081.00
|
|
| RC209429L1 | Lenti ORF clone of Human signal transducer and activator of transcription 5B (STAT5B), Myc-DDK-tagged | 10 ug |
$1,381.00
|
|
| RC209429L2 | Lenti ORF clone of Human signal transducer and activator of transcription 5B (STAT5B), mGFP tagged | 10 ug |
$1,381.00
|
|
| RC209429L3 | Lenti ORF clone of Human signal transducer and activator of transcription 5B (STAT5B), Myc-DDK-tagged | 10 ug |
$1,381.00
|
|
| RC209429L4 | Lenti ORF clone of Human signal transducer and activator of transcription 5B (STAT5B), mGFP tagged | 10 ug |
$1,381.00
|
|
| SC115355 | STAT5B (untagged)-Human signal transducer and activator of transcription 5B (STAT5B) | 10 ug |
$1,082.00
|
|
Citations
Antagonizing STAT5B dimerization with an osmium complex
Liu, Wang, Kang et al
Sci Rep (2016) 6, 36044 DOI: 10.1038/srep36044
Liu, Wang, Kang et al
Sci Rep (2016) 6, 36044 DOI: 10.1038/srep36044
AI Summary: OriGene products contributed to this study by providing the pCMV6-AC-GFP-STAT5B plasmid and anti-GFP antibody, which were used to express and detect STAT5B in experiments investigating the inhibition of STAT5B dimerization by an osmium complex.
Snippet: m, pGL-STAT5 and pRL-TK vector were obtained from Promega Corporation (Promega, Madison, WI, USA). The plasmid encoding with STAT5B gene (pCMV6-AC-GFP-STAT5B) and anti-GFP antibody were purchased from OriGene Technologies (Rockville, MD, USA). Prolactin, ANTI-FLAG®M2 Magnetic Beads,
The oncogenic FIP1L1-PDGFRα fusion protein displays skewed signaling properties compared to its wild-type PDGFRα counterpart
Haan, Bahlawane, Wang et al
JAKSTAT (2015) 4 (1), e1062596 DOI: 10.1080/21623996.2015.1062596
Haan, Bahlawane, Wang et al
JAKSTAT (2015) 4 (1), e1062596 DOI: 10.1080/21623996.2015.1062596
Cited by 5
The oncogenic FIP1L1-PDGFRα fusion protein displays skewed signaling properties compared to its wild-type PDGFRα counterpart
Haan, Bahlawane, Wang et al
JAK-STAT (2015) 4, e1062596
Haan, Bahlawane, Wang et al
JAK-STAT (2015) 4, e1062596
Snippet: pCMV-AC-GFP-STAT5B expression construct was purchased from OriGene (CatNo. RG209429). FIP1L1-PDGFRα sequence was extracted from EOL-1 cells. Cellular RNA was isolated using the RNeasy Mini Kit (QIAGEN, 74104) and cDNA was prepared using 1st Strand cDNA Synthesis Kit for RT-PCR (Roche Applied Science, 11483188001)
Showing 1-3 of 3 papers.
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