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IBRDC2 (RNF144B) (NM_182757) Human Tagged ORF Clone

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SKU
RC209302
RNF144B (Myc-DDK-tagged)-Human ring finger protein 144B (RNF144B)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IBRDC2
Synonyms bA528A10.3; IBRDC2; p53RFP; PIR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209302 representing NM_182757
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTCAGCTGGTAGGCTCCACTATCTCGCCATGACTGCTGAAAATCCCACTCCTGGAGACCTGGCTC
CGGCCCCCCTCATCACTTGCAAACTCTGCCTGTGTGAGCAGTCTCTGGACAAGATGACCACACTCCAGGA
ATGCCAGTGCATCTTTTGCACAGCTTGCCTGAAACAGTACATGCAGCTGGCAATCCGAGAAGGATGTGGG
TCTCCCATCACTTGCCCTGACATGGTGTGCCTAAACCACGGGACCCTGCAGGAAGCTGAGATTGCCTGTT
TGGTACCTGTGGACCAGTTTCAACTTTATCAGAGGTTAAAATTTGAAAGAGAAGTTCATCTGGACCCCTA
CCGAACATGGTGTCCTGTTGCAGACTGTCAGACAGTGTGCCCTGTTGCCTCGAGTGACCCAGGACAGCCT
GTGCTGGTGGAATGCCCTTCTTGCCACCTGAAATTCTGCTCGTGTTGCAAGGATGCTTGGCATGCAGAGG
TCTCCTGTAGAGACAGTCAGCCTATTGTCCTGCCAACAGAGCACCGAGCCCTCTTTGGGACAGATGCAGA
AGCCCCCATTAAGCAGTGCCCAGTTTGCCGGGTTTATATCGAACGCAATGAAGGCTGCGCTCAGATGATG
TGCAAAAACTGCAAGCATACATTTTGCTGGTACTGCCTTCAGAACTTGGATAATGACATTTTCCTCAGAC
ATTATGACAAAGGGCCATGCAGGAATAAACTTGGCCACTCAAGAGCATCAGTGATGTGGAACCGAACACA
GGTGGTGGGGATTCTCGTAGGCTTGGGCATCATTGCCTTGGTTACTTCCCCCTTACTCCTGGCCTCCCCA
TGTATAATCTGTTGTGTCTGCAAGTCCTGTCGGGGCAAGAAGAAAAAGCACGACCCATCCACAACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209302 representing NM_182757
Red=Cloning site Green=Tags(s)

MGSAGRLHYLAMTAENPTPGDLAPAPLITCKLCLCEQSLDKMTTLQECQCIFCTACLKQYMQLAIREGCG
SPITCPDMVCLNHGTLQEAEIACLVPVDQFQLYQRLKFEREVHLDPYRTWCPVADCQTVCPVASSDPGQP
VLVECPSCHLKFCSCCKDAWHAEVSCRDSQPIVLPTEHRALFGTDAEAPIKQCPVCRVYIERNEGCAQMM
CKNCKHTFCWYCLQNLDNDIFLRHYDKGPCRNKLGHSRASVMWNRTQVVGILVGLGIIALVTSPLLLASP
CIICCVCKSCRGKKKKHDPSTT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182757
ORF Size 906 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182757.1
RefSeq Size 4782 bp
RefSeq ORF 912 bp
Locus ID 255488
UniProt ID Q7Z419
Cytogenetics 6p22.3
Protein Families Transmembrane
MW 33.5 kDa
Summary E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.UniProtKB/Swiss-Prot Function
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"NM_182757" in other vectors (6)
SKU Description Size Price
RC209302L1 Lenti ORF clone of Human ring finger protein 144B (RNF144B), Myc-DDK-tagged 10 ug
$600.00
RC209302L2 Lenti ORF clone of Human ring finger protein 144B (RNF144B), mGFP tagged 10 ug
$600.00
RC209302L3 Lenti ORF clone of Human ring finger protein 144B (RNF144B), Myc-DDK-tagged 10 ug
$600.00
RC209302L4 Lenti ORF clone of Human ring finger protein 144B (RNF144B), mGFP tagged 10 ug
$600.00
RG209302 RNF144B (tGFP-tagged) - Human ring finger protein 144B (RNF144B) 10 ug
$489.00 $500.00
SC121747 RNF144B (untagged)-Human ring finger protein 144B (RNF144B) 10 ug
$300.00

Plasmid Map for RC209302

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209302
Circular map for RC209302

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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