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IBRDC2 (RNF144B) (NM_182757) Human Untagged Clone

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SKU
SC121747
RNF144B (untagged)-Human ring finger protein 144B (RNF144B)
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol IBRDC2
Synonyms bA528A10.3; IBRDC2; p53RFP; PIR2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC121747 sequence for NM_182757 edited (data generated by NextGen Sequencing)
ATGGGCTCAGCTGGTAGGCTCCACTATCTCGCCATGACTGCTGAAAATCCCACTCCTGGA
GACCTGGCTCCGGCCCCCCTCATCACTTGCAAACTCTGCCTGTGTGAGCAGTCTCTGGAC
AAGATGACCACACTCCAGGAATGCCAGTGCATCTTTTGCACAGCTTGCCTGAAACAGTAC
ATGCAGCTGGCAATCCGAGAAGGATGTGGGTCTCCCATCACTTGCCCTGACATGGTGTGC
CTAAACCACGGGACCCTGCAGGAAGCTGAGATTGCCTGTTTGGTACCTGTGGACCAGTTT
CAACTTTATCAGAGGTTAAAATTTGAAAGAGAAGTTCATCTGGACCCCTACCGAACATGG
TGTCCTGTTGCAGACTGTCAGACAGTGTGCCCTGTTGCCTCGAGTGACCCAGGACAGCCT
GTGCTGGTGGAATGCCCTTCTTGCCACCTGAAATTCTGCTCGTGTTGCAAGGATGCTTGG
CATGCAGAGGTCTCCTGTAGAGACAGTCAGCCTATTGTCCTGCCAACAGAGCACCGAGCC
CTCTTTGGGACAGATGCAGAAGCCCCCATTAAGCAGTGCCCAGTTTGCCGGGTTTATATC
GAACGCAATGAAGGCTGCGCTCAGATGATGTGCAAAAACTGCAAGCATACATTTTGCTGG
TACTGCCTCCAGAACTTGGATAATGACATTTTCCTCAGACATTATGACAAAGGGCCATGC
AGGAATAAACTTGGCCACTCAAGAGCATCAGTGATGTGGAACCGAACACAGGTGGTGGGG
ATTCTCGTAGGCTTGGGCATCATTGCCTTGGTTACTTCACCCTTGTTACTCCTGGCCTCC
CCATGTATAATCTGTTGTGTCTGCAAGTCCTGTCGGGGCAAGAAGAAAAAGCACGACCCA
TCCACAACCTAA

Clone variation with respect to NM_182757.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_182757 unedited
GCCGCGAATTCGGCACGAGCAGAGCACGGAGGAAAGACGGAGAGAATGGAAGAGCTCCTG
TCCGGTGTGCCAGCAGCCCGGACTGGCGGTGAGCGCGAGGGAGGCTACTGAGAAGCCCGG
CGACGGAGGAACGCAGGTCTGCTGCCAGGGATTGAGGAGACTGAAGAACGCTGAAGACAG
GCTGATGGGCTCAGCTGGTAGGCTCCACTATCTCGCCATGACTGCTGAAAATCCCACTCC
TGGAGACCTGGCTCCGGCCCCCCTCATCACTTGCAAACTCTGCCTGTGTGAGCAGTCTCT
GGACAAGATGACCACACTCCAGGAATGCCAGTGCATCTTTTGCACAGCTTGCCTGAAACA
GTACATGCAGCTGGCAATCCGAGAAGGATGTGGGTCTCCCATCACTTGCCCTGACATGGT
GTGCCTAAACCACGGGACCCTGCAGGAAGCTGAGATTGCCTGTTTGGTACCTGTGGACCA
GTTTCAACTTTATCAGAGGTTAAAATTTGAAAGAGAAGTTCATCTGGACCCCTACCGAAC
ATGGTGTCCTGTTGCAGACTGTCAGACAGTGTGCCCTGTTGCCTCGAGTGACCCAGGACA
GCCTGTGCTGGTGGAATGCCCTTCTTGCCACCTGAAATTCTGCTCGTGTTGCAAGGATGC
TTGGCATGCANAGGTCTNCTGTANAGACAGTCAGCCTATTGTCCTGCCACAGAGCACCGA
GCCCTCTTTGGGACAGATGCAGAAGCCCC
Restriction Sites NotI-NotI |
ACCN NM_182757
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182757.2, NP_877434.2
RefSeq Size 4782 bp
RefSeq ORF 912 bp
Locus ID 255488
UniProt ID Q7Z419
Cytogenetics 6p22.3
Protein Families Transmembrane
Summary E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.UniProtKB/Swiss-Prot Function
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"NM_182757" in other vectors (6)
SKU Description Size Price
RC209302 RNF144B (Myc-DDK-tagged)-Human ring finger protein 144B (RNF144B) 10 ug
$300.00
RC209302L1 Lenti ORF clone of Human ring finger protein 144B (RNF144B), Myc-DDK-tagged 10 ug
$600.00
RC209302L2 Lenti ORF clone of Human ring finger protein 144B (RNF144B), mGFP tagged 10 ug
$600.00
RC209302L3 Lenti ORF clone of Human ring finger protein 144B (RNF144B), Myc-DDK-tagged 10 ug
$600.00
RC209302L4 Lenti ORF clone of Human ring finger protein 144B (RNF144B), mGFP tagged 10 ug
$600.00
RG209302 RNF144B (tGFP-tagged) - Human ring finger protein 144B (RNF144B) 10 ug
$489.00 $500.00

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