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KRTCAP2 (NM_173852) Human Tagged ORF Clone

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SKU
RC208887
KRTCAP2 (Myc-DDK-tagged)-Human keratinocyte associated protein 2 (KRTCAP2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "KRTCAP2 or NM_173852"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KRTCAP2
Synonyms KCP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208887 representing NM_173852
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCATAGCTAACCGCACCCGGTTCAGCTCGCCTTTCTTGGCCAGAGGCGCCGGTTGGACTCACGGGC
GGGGCATGATGGTGGTGGGTACGGGCACCTCGCTGGCGCTCTCCTCCCTCCTGTCCCTGCTGCTCTTTGC
TGGGATGCAGATGTACAGCCGTCAGCTGGCCTCCACCGAGTGGCTCACCATCCAGGGCGGCCTGCTTGGT
TCGGGTCTCTTCGTGTTCTCGCTCACTGCCTTCAATAATCTGGAGAATCTTGTCTTTGGCAAAGGATTCC
AAGCAAAGATCTTCCCTGAGATTCTCCTGTGCCTCCTGTTGGCTCTCTTTGCATCTGGCCTCATCCACCG
AGTCTGTGTCACCACCTGCTTCATCTTCTCCATGGTTGGTCTGTACTACATCAACAAGATCTCCTCCACC
CTGTACCAGGCAGCAGCTCCAGTCCTCACACCAGCCAAGGTCACAGGCAAGAGCAAGAAGAGAAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208887 representing NM_173852
Red=Cloning site Green=Tags(s)

MRIANRTRFSSPFLARGAGWTHGRGMMVVGTGTSLALSSLLSLLLFAGMQMYSRQLASTEWLTIQGGLLG
SGLFVFSLTAFNNLENLVFGKGFQAKIFPEILLCLLLALFASGLIHRVCVTTCFIFSMVGLYYINKISST
LYQAAAPVLTPAKVTGKSKKRN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173852
ORF Size 486 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173852.3, NP_776251.1
RefSeq Size 577 bp
RefSeq ORF 411 bp
Locus ID 200185
UniProt ID Q8N6L1
Cytogenetics 1q22
Protein Families Transmembrane
MW 17.4 kDa
Summary Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (PubMed:22467853). May be involved in N-glycosylation of APP (amyloid-beta precursor protein). Can modulate gamma-secretase cleavage of APP by enhancing endoprotelysis of PSEN1 (PubMed:21768116).UniProtKB/Swiss-Prot Function
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"NM_173852" in other vectors (7)
SKU Description Size Price
RC208887L1 Lenti ORF clone of Human keratinocyte associated protein 2 (KRTCAP2), Myc-DDK-tagged 10 ug
$450.00
RC208887L2 Lenti ORF clone of Human keratinocyte associated protein 2 (KRTCAP2), mGFP tagged 10 ug
$450.00
RC208887L3 Lenti ORF clone of Human keratinocyte associated protein 2 (KRTCAP2), Myc-DDK-tagged 10 ug
$450.00
RC208887L4 Lenti ORF clone of Human keratinocyte associated protein 2 (KRTCAP2), mGFP tagged 10 ug
$450.00
RG208887 KRTCAP2 (tGFP-tagged) - Human keratinocyte associated protein 2 (KRTCAP2) 10 ug
$350.00
SC101215 KRTCAP2 (untagged)-Human keratinocyte associated protein 2 (KRTCAP2) 10 ug
$150.00
SC320977 KRTCAP2 (untagged)-Human keratinocyte associated protein 2 (KRTCAP2) 10 ug
$150.00

Plasmid Map for RC208887

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208887
Circular map for RC208887

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