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LEPROT (NM_017526) Human Tagged ORF Clone

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SKU
RC208878
LEPROT (Myc-DDK-tagged)-Human leptin receptor overlapping transcript (LEPROT), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "LEPROT or NM_017526"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LEPROT
Synonyms LEPR; OB-RGRP; OBRGRP; VPS55
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208878 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCGTTAAAGCTCTCGTGGCATTATCCTTCAGTGGGGCTATTGGACTGACTTTTCTTATGCTGG
GATGTGCCTTAGAGGATTATGGCGTTTACTGGCCCTTATTCGTCCTGATTTTCCACGCCATCTCCCCCAT
CCCCCATTTCATTGCCAAAAGAGTCACCTATGACTCAGATGCAACCAGTAGTGCCTGTCGGGAACTGGCA
TATTTCTTCACTACTGGAATTGTTGTTTCTGCCTTTGGATTTCCTGTTATTCTTGCTCGTGTGGCTGTGA
TCAAATGGGGAGCCTGCGGCCTTGTGTTGGCAGGCAATGCAGTCATTTTCCTTACAATTCAAGGGTTTTT
CCTTATATTTGGAAGAGGAGATGATTTTAGCTGGGAGCAGTGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208878 protein sequence
Red=Cloning site Green=Tags(s)

MAGVKALVALSFSGAIGLTFLMLGCALEDYGVYWPLFVLIFHAISPIPHFIAKRVTYDSDATSSACRELA
YFFTTGIVVSAFGFPVILARVAVIKWGACGLVLAGNAVIFLTIQGFFLIFGRGDDFSWEQW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017526
ORF Size 393 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017526.5
RefSeq Size 4777 bp
RefSeq ORF 396 bp
Locus ID 54741
UniProt ID O15243
Cytogenetics 1p31.3
Domains Vps55
Protein Families Druggable Genome, Transmembrane
MW 14.3 kDa
Summary LEPROT is associated with the Golgi complex and endosomes and has a role in cell surface expression of growth hormone receptor (GHR; MIM 600946) and leptin receptor (OBR, or LEPR; MIM 601007), thereby altering receptor-mediated cell signaling (Couturier et al., 2007 PubMed 18042720; Touvier et al., 2009 PubMed 19907080).supplied by OMIM, Jul 2010
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"NM_017526" in other vectors (6)
SKU Description Size Price
RC208878L1 Lenti ORF clone of Human leptin receptor overlapping transcript (LEPROT), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC208878L2 Lenti ORF clone of Human leptin receptor overlapping transcript (LEPROT), transcript variant 1, mGFP tagged 10 ug
$450.00
RC208878L3 Lenti ORF clone of Human leptin receptor overlapping transcript (LEPROT), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC208878L4 Lenti ORF clone of Human leptin receptor overlapping transcript (LEPROT), transcript variant 1, mGFP tagged 10 ug
$450.00
RG208878 LEPROT (tGFP-tagged) - Human leptin receptor overlapping transcript (LEPROT), transcript variant 1 10 ug
$489.00
SC127307 LEPROT (untagged)-Human leptin receptor overlapping transcript (LEPROT), transcript variant 1 10 ug
$150.00

Plasmid Map for RC208878

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208878
Circular map for RC208878

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