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LEPROT (NM_017526) Human Untagged Clone

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SKU
SC127307
LEPROT (untagged)-Human leptin receptor overlapping transcript (LEPROT), transcript variant 1
  $150.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol LEPROT
Synonyms LEPR; OB-RGRP; OBRGRP; VPS55
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC127307 sequence for NM_017526 edited (data generated by NextGen Sequencing)
ATGGCGGGCGTTAAAGCTCTCGTGGCATTATCCTTCAGTGGGGCTATTGGACTGACTTTT
CTTATGCTGGGATGTGCCTTAGAGGATTATGGCGTTTACTGGCCCTTATTCGTCCTGATT
TTCCACGCCATCTCCCCCATCCCCCATTTCATTGCCAAAAGAGTCACCTATGACTCAGAT
GCAACCAGTAGTGCCTGTCGGGAACTGGCATATTTCTTCACTACTGGAATTGTTGTTTCT
GCCTTTGGATTTCCTGTTATTCTTGCTCGTGTGGCTGTGATCAAATGGGGAGCCTGCGGC
CTTGTGTTGGCAGGCAATGCAGTCATTTTCCTTACAATTCAAGGGTTTTTCCTTATATTT
GGAAGAGGAGATGATTTTAGCTGGGAGCAGTGGTAG

Clone variation with respect to NM_017526.4
5' Read Nucleotide Sequence
>OriGene 5' read for NM_017526 unedited
AATATCCCCGCCCGTTGACGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
CAGAGCTCATTTAGGTGACACTATAGAATACAGGCTACTTGTTCTTTTTGCAGCGGCCGC
GAATTCGGCACGAGGGCTGCCCGGGCCGTGGCAGGAAGCCGGAAGCAGCCGCGGCCCCAG
TTCGGGAGACATGGCGGGCGTTAAAGCTCTCGTGGCATTATCCTTCAGTGGGGCTATTGG
ACTGACTTTTCTTATGCTGGGATGTGCCTTAGAGGATTATGGCGTTTACTGGCCCTTATT
CGTCCTGAGTTTCCACGCCATCTCCCCCATCCCCCATTTCATTGCCAAAAGAGTCACCTA
TGACTCAGATGCAACCAGTAGTGCCTGTCGGGAACTGGCATATTTCTTCACTACTGGAAT
TGTTGTTTCTGCCTTTGGATTTCCTGTTATTCTTGCTCGTGTGGCTGTGATCAAATGGGG
AGCCTGCGGCCTTGTGTTGGCAGGCAATGCAGTCATTTTCCTTACAATTCACGGGTTTTT
CCTTATATTTGGAAGAGGAGATGATTTTAGCTGGGAGCAGTGGTAGCACTTTATTCTGAG
TACAGTGCATTGAATTTCTTAGAACTCATACTATCTGTATACATGTGCACATGCGGCATT
TTACTATGAAATTTAATATGCTGGGTTTTTTAATACCTTTATATATCATGTTCACTTTCA
GAGAGACTTCATAAGTAGGAGATGAGTTNTATTCTCAGCAGATAGACCTGTCACATTTAG
ATTATGTTACTCACAGTATGTTACTTGTTTGGCTGNTCATGTAGTCACGGTGCTCTCAGA
GCAATATCACGCAGTCTTGTAGGCAGCTGCCACATATGCAGTGCATCGAAACTTTTGCTT
GGGGATGTGCTTGNAGAGGCGATACGCTGAGC
Restriction Sites NotI-NotI |
ACCN NM_017526
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017526.2, NP_059996.1
RefSeq Size 1147 bp
RefSeq ORF 396 bp
Locus ID 54741
UniProt ID O15243
Cytogenetics 1p31.3
Domains Vps55
Protein Families Druggable Genome, Transmembrane
Summary LEPROT is associated with the Golgi complex and endosomes and has a role in cell surface expression of growth hormone receptor (GHR; MIM 600946) and leptin receptor (OBR, or LEPR; MIM 601007), thereby altering receptor-mediated cell signaling (Couturier et al., 2007 PubMed 18042720; Touvier et al., 2009 PubMed 19907080).supplied by OMIM, Jul 2010
Transcript Variant: This variant (1) is dominant and encodes the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_017526" in other vectors (6)
SKU Description Size Price
RC208878 LEPROT (Myc-DDK-tagged)-Human leptin receptor overlapping transcript (LEPROT), transcript variant 1 10 ug
$150.00
RC208878L1 Lenti ORF clone of Human leptin receptor overlapping transcript (LEPROT), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC208878L2 Lenti ORF clone of Human leptin receptor overlapping transcript (LEPROT), transcript variant 1, mGFP tagged 10 ug
$450.00
RC208878L3 Lenti ORF clone of Human leptin receptor overlapping transcript (LEPROT), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC208878L4 Lenti ORF clone of Human leptin receptor overlapping transcript (LEPROT), transcript variant 1, mGFP tagged 10 ug
$450.00
RG208878 LEPROT (tGFP-tagged) - Human leptin receptor overlapping transcript (LEPROT), transcript variant 1 10 ug
$489.00

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