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CLK2 (NM_003993) Human Tagged ORF Clone

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SKU
RC208778
CLK2 (Myc-DDK-tagged)-Human CDC-like kinase 2 (CLK2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "CLK2 or NM_003993"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CLK2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208778 representing NM_003993
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCATCCTCGAAGGTACCACTCCTCAGAGCGAGGCAGCCGGGGGAGTTACCGTGAACACTATCGGA
GCCGAAAGCATAAGCGACGAAGAAGTCGCTCCTGGTCAAGTAGTAGTGACCGGACACGACGGCGTCGGCG
AGAGGACAGCTACCATGTCCGTTCTCGAAGCAGTTATGATGATCGTTCGTCCGACCGGAGGGTGTATGAC
CGGCGATACTGTGGCAGCTACAGACGCAACGATTATAGCCGGGATCGGGGAGATGCCTACTATGACACAG
ACTATCGGCATTCCTATGAATATCAGCGGGAGAACAGCAGTTACCGCAGCCAGCGCAGCAGCCGGAGGAA
GCACAGACGGCGGAGGAGGCGCAGCCGGACATTTAGCCGCTCATCTTCGCAGCACAGCAGCCGGAGAGCC
AAGAGTGTAGAGGACGACGCTGAGGGCCACCTCATCTACCACGTCGGGGACTGGCTACAAGAGCGATATG
AAATCGTTAGCACCTTAGGAGAGGGGACCTTCGGCCGAGTTGTACAATGTGTTGACCATCGCAGGGGTGG
GGCTCGAGTTGCCCTGAAGATCATTAAGAATGTGGAGAAGTACAAGGAAGCAGCTCGACTTGAGATCAAC
GTGCTAGAGAAAATCAATGAGAAAGACCCTGACAACAAGAACCTCTGTGTCCAGATGTTTGACTGGTTTG
ACTACCATGGCCACATGTGTATCTCCTTTGAGCTTCTGGGCCTTAGCACCTTCGATTTCCTCAAAGACAA
CAACTACCTGCCCTACCCCATCCACCAAGTGCGCCACATGGCCTTCCAGCTGTGCCAGGCTGTCAAGTTC
CTCCATGATAACAAGCTGACACATACAGACCTCAAGCCTGAAAATATTCTGTTTGTGAATTCAGACTATG
AGCTCACCTACAACCTAGAGAAGAAGCGAGATGAGCGCAGTGTGAAGAGCACAGCTGTGCGGGTGGTAGA
CTTTGGCAGTGCCACCTTTGACCATGAGCACCATAGCACCATTGTCTCCACTCGCCATTACCGAGCACCA
GAAGTCATCCTTGAGTTGGGCTGGTCACAGCCTTGTGATGTGTGGAGTATAGGCTGCATCATCTTTGAAT
ACTATGTGGGATTCACCCTCTTCCAGACCCATGACAACAGAGAGCATCTAGCCATGATGGAAAGGATCTT
GGGTCCTATCCCTTCCCGGATGATCCGAAAGACAAGAAAGCAGAAATATTTTTACCGGGGTCGCCTGGAT
TGGGATGAGAACACATCAGCTGGGCGCTATGTTCGTGAGAACTGCAAACCGCTGCGGCGGTATCTGACCT
CAGAGGCAGAGGAACACCACCAGCTCTTCGATCTGATTGAAAGCATGCTAGAGTATGAACCAGCTAAGCG
GCTGACCTTGGGTGAAGCCCTTCAGCATCCTTTCTTCGCCCGCCTTCGGGCTGAGCCGCCCAACAAGTTG
TGGGACTCCAGTCGGGATATCAGTCGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208778 representing NM_003993
Red=Cloning site Green=Tags(s)

MPHPRRYHSSERGSRGSYREHYRSRKHKRRRSRSWSSSSDRTRRRRREDSYHVRSRSSYDDRSSDRRVYD
RRYCGSYRRNDYSRDRGDAYYDTDYRHSYEYQRENSSYRSQRSSRRKHRRRRRRSRTFSRSSSQHSSRRA
KSVEDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKEAARLEIN
VLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQAVKF
LHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAP
EVILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPIPSRMIRKTRKQKYFYRGRLD
WDENTSAGRYVRENCKPLRRYLTSEAEEHHQLFDLIESMLEYEPAKRLTLGEALQHPFFARLRAEPPNKL
WDSSRDISR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003993
ORF Size 1497 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003993.1
RefSeq Size 2175 bp
RefSeq ORF 1497 bp
Locus ID 1196
UniProt ID P49760
Cytogenetics 1q22
Domains pkinase, S_TKc, TyrKc
Protein Families Druggable Genome, Protein Kinase
MW 59.8 kDa
Summary This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jun 2014
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Documents
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"NM_003993" in other vectors (7)
SKU Description Size Price
RC208778L1 Lenti ORF clone of Human CDC-like kinase 2 (CLK2), Myc-DDK-tagged 10 ug
$757.00
RC208778L2 Lenti ORF clone of Human CDC-like kinase 2 (CLK2), mGFP tagged 10 ug
$757.00
RC208778L3 Lenti ORF clone of Human CDC-like kinase 2 (CLK2), Myc-DDK-tagged 10 ug
$757.00
RC208778L4 Lenti ORF clone of Human CDC-like kinase 2 (CLK2), mGFP tagged 10 ug
$757.00
RG208778 CLK2 (tGFP-tagged) - Human CDC-like kinase 2 (CLK2) 10 ug
$489.00 $657.00
SC128171 CLK2 (untagged)-Human CDC-like kinase 2 (CLK2) 10 ug
$457.00
SC323449 CLK2 (untagged)-Kinase deficient mutant (K192M) of Human CDC-like kinase 2 (CLK2) 10 ug
$457.00

Plasmid Map for RC208778

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208778
Circular map for RC208778

file-alt Citations

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