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LIN7C (NM_018362) Human Tagged ORF Clone

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SKU
RC208758
LIN7C (Myc-DDK-tagged)-Human lin-7 homolog C (C. elegans) (LIN7C)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LIN7C
Synonyms LIN-7-C; LIN-7C; MALS-3; MALS3; VELI3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208758 representing NM_018362
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCTAGGGGAACCCGTGCGGCTGGAGAGAGATATTTGTAGAGCAATTGAATTATTGGAAAAAC
TACAAAGGAGTGGAGAAGTACCACCACAGAAACTTCAGGCTTTGCAAAGAGTCCTTCAAAGTGAATTCTG
CAATGCTGTGAGAGAGGTATATGAACATGTCTATGAGACTGTGGACATCAGTAGCAGTCCTGAAGTGAGA
GCGAACGCTACTGCAAAGGCTACTGTTGCTGCATTTGCTGCCAGTGAAGGACATTCTCATCCTCGAGTTG
TTGAGCTACCAAAAACAGAAGAGGGCCTTGGATTCAATATTATGGGAGGCAAAGAACAAAACTCTCCAAT
CTATATATCCCGAATAATTCCAGGTGGAATTGCTGATAGACATGGGGGCCTCAAACGTGGAGATCAACTC
CTCTCTGTTAATGGAGTGAGTGTTGAAGGAGAACATCATGAAAAAGCTGTAGAACTGCTGAAAGCCGCAC
AAGGAAAGGTTAAATTAGTGGTACGATACACACCCAAAGTCTTAGAAGAAATGGAGTCGCGCTTTGAAAA
AATGAGATCAGCAAAACGCAGGCAACAGACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208758 representing NM_018362
Red=Cloning site Green=Tags(s)

MAALGEPVRLERDICRAIELLEKLQRSGEVPPQKLQALQRVLQSEFCNAVREVYEHVYETVDISSSPEVR
ANATAKATVAAFAASEGHSHPRVVELPKTEEGLGFNIMGGKEQNSPIYISRIIPGGIADRHGGLKRGDQL
LSVNGVSVEGEHHEKAVELLKAAQGKVKLVVRYTPKVLEEMESRFEKMRSAKRRQQT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018362
ORF Size 591 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018362.4
RefSeq Size 4729 bp
RefSeq ORF 594 bp
Locus ID 55327
UniProt ID Q9NUP9
Cytogenetics 11p14.1
Domains L27, PDZ
Protein Families Druggable Genome
MW 21.7 kDa
Summary Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.UniProtKB/Swiss-Prot Function
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"NM_018362" in other vectors (6)
SKU Description Size Price
RC208758L1 Lenti ORF clone of Human lin-7 homolog C (C. elegans) (LIN7C), Myc-DDK-tagged 10 ug
$600.00
RC208758L2 Lenti ORF clone of Human lin-7 homolog C (C. elegans) (LIN7C), mGFP tagged 10 ug
$600.00
RC208758L3 Lenti ORF clone of Human lin-7 homolog C (C. elegans) (LIN7C), Myc-DDK-tagged 10 ug
$600.00
RC208758L4 Lenti ORF clone of Human lin-7 homolog C (C. elegans) (LIN7C), mGFP tagged 10 ug
$600.00
RG208758 LIN7C (tGFP-tagged) - Human lin-7 homolog C (C. elegans) (LIN7C) 10 ug
$500.00
SC127489 LIN7C (untagged)-Human lin-7 homolog C (C. elegans) (LIN7C) 10 ug
$300.00

Plasmid Map for RC208758

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208758
Circular map for RC208758

file-alt Citations

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