SGPL1 (NM_003901) Human Tagged ORF Clone

SKU RC208705

SGPL1 (Myc-DDK-tagged)-Human sphingosine-1-phosphate lyase 1 (SGPL1)

Product Data

• Target Symbol: SGPL1
• Synonyms: NPHS14; S1PL; SPL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208705 representing NM_003901; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCTAGCACAGACCTTCTGATGTTGAAGGCCTTTGAGCCCTACTTAGAGATTTTGGAAGTATACTCCA; CAAAAGCCAAGAATTATGTAAATGGACATTGCACCAAGTATGAGCCCTGGCAGCTAATTGCATGGAGTGT; CGTGTGGACCCTGCTGATAGTCTGGGGATATGAGTTTGTCTTCCAGCCAGAGAGTTTATGGTCAAGGTTT; AAAAAGAAATGTTTTAAGCTCACCAGGAAGATGCCCATTATTGGTCGTAAGATTCAAGACAAGTTGAACA; AGACCAAGGATGATATTAGCAAGAACATGTCATTCCTGAAAGTGGACAAAGAGTATGTGAAAGCTTTACC; CTCCCAGGGTCTGAGCTCATCTGCTGTTTTGGAGAAACTTAAGGAGTACAGCTCTATGGACGCCTTCTGG; CAAGAGGGGAGAGCCTCTGGAACAGTGTACAGTGGGGAGGAGAAGCTCACTGAGCTCCTTGTGAAGGCTT; ATGGAGATTTTGCATGGAGTAACCCCCTGCATCCAGATATCTTCCCAGGACTACGCAAGATAGAGGCAGA; AATTGTGAGGATAGCTTGTTCCCTGTTCAATGGGGGACCAGATTCGTGTGGATGTGTGACTTCTGGGGGA; ACAGAAAGCATACTGATGGCCTGCAAAGCATATCGGGATCTGGCCTTTGAGAAGGGGATCAAAACTCCAG; AAATTGTGGCTCCCCAAAGTGCCCATGCTGCATTTAACAAAGCAGCCAGTTACTTTGGGATGAAGATTGT; GCGGGTCCCATTGACGAAGATGATGGAGGTGGATGTGCGGGCAATGAGAAGAGCTATCTCCAGGAACACT; GCCATGCTCGTCTGTTCTACCCCACAGTTTCCTCATGGTGTAATAGATCCTGTCCCTGAAGTGGCCAAGC; TGGCTGTCAAATACAAAATACCCCTTCATGTCGACGCTTGTCTGGGAGGCTTCCTCATCGTCTTTATGGA; GAAAGCAGGATACCCACTGGAGCACCCATTTGATTTCCGGGTGAAAGGTGTAACCAGCATTTCAGCTGAC; ACCCATAAGTATGGCTATGCCCCAAAAGGCTCATCATTGGTGTTGTATAGTGACAAGAAGTACAGGAACT; ATCAGTTCTTCGTCGATACAGATTGGCAGGGTGGCATCTATGCTTCCCCAACCATCGCAGGCTCACGGCC; TGGTGGCATTAGCGCAGCCTGTTGGGCTGCCTTGATGCACTTCGGTGAGAACGGCTATGTTGAAGCTACC; AAACAGATCATCAAAACTGCTCGCTTCCTCAAGTCAGAACTGGAAAATATCAAAGGCATCTTTGTTTTTG; GGAATCCCCAATTGTCAGTCATTGCTCTGGGATCCCGTGATTTTGACATCTACCGACTATCAAACCTGAT; GACTGCTAAGGGGTGGAACTTGAACCAGTTGCAGTTCCCACCCAGTATTCATTTCTGCATCACATTACTA; CACGCCCGGAAACGAGTAGCTATACAATTCCTAAAGGACATTCGAGAATCTGTCACTCAAATCATGAAGA; ATCCTAAAGCGAAGACCACAGGAATGGGTGCCATCTATGGCATGGCCCAGACAACTGTTGACAGGAATAT; GGTTGCAGAATTGTCCTCAGTCTTCTTGGACAGCTTGTACAGCACCGACACTGTCACCCAGGGCAGCCAG; ATGAATGGTTCTCCAAAACCCCAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208705 representing NM_003901; Red=Cloning site Green=Tags(s); ; MPSTDLLMLKAFEPYLEILEVYSTKAKNYVNGHCTKYEPWQLIAWSVVWTLLIVWGYEFVFQPESLWSRF; KKKCFKLTRKMPIIGRKIQDKLNKTKDDISKNMSFLKVDKEYVKALPSQGLSSSAVLEKLKEYSSMDAFW; QEGRASGTVYSGEEKLTELLVKAYGDFAWSNPLHPDIFPGLRKIEAEIVRIACSLFNGGPDSCGCVTSGG; TESILMACKAYRDLAFEKGIKTPEIVAPQSAHAAFNKAASYFGMKIVRVPLTKMMEVDVRAMRRAISRNT; AMLVCSTPQFPHGVIDPVPEVAKLAVKYKIPLHVDACLGGFLIVFMEKAGYPLEHPFDFRVKGVTSISAD; THKYGYAPKGSSLVLYSDKKYRNYQFFVDTDWQGGIYASPTIAGSRPGGISAACWAALMHFGENGYVEAT; KQIIKTARFLKSELENIKGIFVFGNPQLSVIALGSRDFDIYRLSNLMTAKGWNLNQLQFPPSIHFCITLL; HARKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQTTVDRNMVAELSSVFLDSLYSTDTVTQGSQ; MNGSPKPH; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003901
• ORF Size: 1704 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003901.2, NP_003892.2
• RefSeq Size: 4701 bp
• RefSeq ORF: 1707 bp
• Locus ID: 8879
• UniProt ID: O95470
• Cytogenetics: 10q22.1
• Domains: pyridoxal_deC
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• MW: 63.3 kDa
• Summary: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis (PubMed:11018465, PubMed:14570870, PubMed:24809814, PubMed:28165339). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts. Involved in the regulation of pro-inflammatory response and neutrophil trafficking. Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (By similarity). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).[UniProtKB/Swiss-Prot Function]