SGPL1 (NM_003901) Human Tagged ORF Clone
$529.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SGPL1 |
| Synonyms | NPHS14; S1PL; SPL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003901 |
| ORF Size | 1704 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003901.2, NP_003892.2 |
| RefSeq Size | 4701 bp |
| RefSeq ORF | 1707 bp |
| Locus ID | 8879 |
| UniProt ID | O95470 |
| Cytogenetics | 10q22.1 |
| Domains | pyridoxal_deC |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| MW | 63.3 kDa |
| Summary | Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis (PubMed:11018465, PubMed:14570870, PubMed:24809814, PubMed:28165339). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts. Involved in the regulation of pro-inflammatory response and neutrophil trafficking. Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (By similarity). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003901" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208705L1 | Lenti ORF clone of Human sphingosine-1-phosphate lyase 1 (SGPL1), Myc-DDK-tagged | 10 ug |
$829.00
|
|
| RC208705L2 | Lenti ORF clone of Human sphingosine-1-phosphate lyase 1 (SGPL1), mGFP tagged | 10 ug |
$829.00
|
|
| RC208705L3 | Lenti ORF clone of Human sphingosine-1-phosphate lyase 1 (SGPL1), Myc-DDK-tagged | 10 ug |
$829.00
|
|
| RC208705L4 | Lenti ORF clone of Human sphingosine-1-phosphate lyase 1 (SGPL1), mGFP tagged | 10 ug |
$829.00
|
|
| RG208705 | SGPL1 (tGFP-tagged) - Human sphingosine-1-phosphate lyase 1 (SGPL1) | 10 ug |
$489.00
$729.00
|
|
| SC308998 | SGPL1 (untagged)-Human sphingosine-1-phosphate lyase 1 (SGPL1) | 10 ug |
$794.00
|
|
Citations
Insights From Long-term Follow-up of a Girl With Adrenal Insufficiency and Sphingosine-1-Phosphate Lyase Deficiency
Maharaj, Güran, Buonocore et al
J Endocr Soc (2022) 6 (5), bvac020 DOI: 10.1210/jendso/bvac020
Maharaj, Güran, Buonocore et al
J Endocr Soc (2022) 6 (5), bvac020 DOI: 10.1210/jendso/bvac020
Cited by 7
AI Summary: OriGene’s human SGPL1 Myc-DDK-tagged ORF cDNA clone (Cat# RC223459) was used to express wild-type and mutant SGPL1 in cell models, enabling the authors to assess the functional impact of the p.D350G variant on protein expression and cortisol production in adrenal cells.
Insights from long term follow-up of a girl with adrenal insufficiency and sphingosine-1-phosphate lyase deficiency
Maharaj, Güran, Buonocore et al
J Endocr Soc (2022) 6 (5), bvac020 DOI: 10.1210/jendso/bvac020
Maharaj, Güran, Buonocore et al
J Endocr Soc (2022) 6 (5), bvac020 DOI: 10.1210/jendso/bvac020
Cited by 7
AI Summary: In this study, the OriGene Human Tagged ORF Clone for SGPL1 (RC208705) was used as the template for site-directed mutagenesis to generate specific SGPL1 variants, enabling functional analysis of the mutant protein’s role in adrenal insufficiency.
Snippet: … Site-directed Mutagenesis Site-directed mutagenesis of an SGPL1 (NM_003901) Human Tagged ORF Clone (ORIGENE, RC208705) was performed using the QuikChange II XL site-directed mutagenesis kit (Agilent, 200521) according to the manufacturer’s instructions. Primers for generation of 4 specific mutants;Site-directed mutagenesis of an SGPL1 (NM_003901) Human Tagged ORF Clone (ORIGENE, RC208705) was performed using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent,
A Sphingosine-1-Phosphate Lyase Mutation Associated With Congenital Nephrotic Syndrome and Multiple Endocrinopathy
Maharaj, Theodorou, Banerjee et al
Front Pediatr (2020) 8, 151 DOI: 10.3389/fped.2020.00151
Maharaj, Theodorou, Banerjee et al
Front Pediatr (2020) 8, 151 DOI: 10.3389/fped.2020.00151
Cited by 25
AI Summary: OriGene's SGPL1 Human Tagged ORF Clone was used to generate mutant SGPL1 constructs via site-directed mutagenesis, enabling the study of protein expression and function in this paper.
Snippet: Site directed mutagenesis of SGPL1 Human Tagged ORF Clone (Origene) and oligonucleotide primer design was performed using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) and web based QuikChange program in keeping with the manufacturer's;- Site directed mutagenesis of SGPL1 Human Tagged ORF Clone (Origene) and oligonucleotide primer design was performed using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) and web based QuikChange program in keeping with the manufacturer's
First evidence of SGPL1 expression in the cell membrane silencing the extracellular S1P siren in mammary epithelial cells
Engel, Adamus, Frank et al
PLoS ONE (2018) 13 (5), e0196854 DOI: 10.1371/journal.pone.0196854
Engel, Adamus, Frank et al
PLoS ONE (2018) 13 (5), e0196854 DOI: 10.1371/journal.pone.0196854
AI Summary: OriGene’s TrueORF clone RC208705, encoding full-length human SGPL1 with a C-terminal DDK tag, was used to produce SGPL1 protein for expression and functional studies in this research.
Snippet: lyase 1 (SGPL1) was produced with TrueORF clone, RC208705 encoding the full-length human SGPL1 with C-terminal DDK tag, from human HEK293 cells (GenBank accession: NM_003901, Predicted molecular weight: 63.3 kDa, purchased from OriGene Technologies Cached
Showing 1-4 of 4 papers.
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