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MTMR2 (NM_016156) Human Tagged ORF Clone

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SKU
RC208703
MTMR2 (Myc-DDK-tagged)-Human myotubularin related protein 2 (MTMR2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $598.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MTMR2
Synonyms CMT4B; CMT4B1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208703 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACGAGCTCGAGCTGCGAGAGTCTTGGCTCCCAGCCGGCGGCGGCTCGGCCGCCCAGCGTGGACT
CCTTGTCCAGTGCCTCCACTTCTCATTCAGAGAATTCAGTGCATACAAAATCAGCTTCTGTTGTATCATC
AGATTCCATTTCAACTTCTGCCGACAACTTTTCTCCTGATTTGAGGGTCCTGAGGGAGTCTAACAAGTTA
GCAGAAATGGAAGAACCACCCTTGCTTCCAGGAGAAAATATTAAAGACATGGCCAAAGATGTAACTTATA
TATGTCCATTCACTGGCGCTGTACGAGGAACTCTGACTGTCACGAATTATAGGTTATATTTCAAAAGCAT
GGAACGGGATCCCCCATTTGTTTTAGATGCTTCCCTTGGTGTGATAAATAGAGTAGAAAAAATTGGTGGT
GCTTCTAGTCGAGGTGAAAATTCTTATGGACTAGAAACTGTGTGTAAGGATATTAGGAATTTACGATTTG
CTCATAAACCTGAGGGGCGGACAAGAAGATCCATATTTGAGAATCTAATGAAATATGCATTTCCTGTCTC
TAATAACCTGCCTCTTTTTGCTTTTGAATACAAAGAAGTATTCCCTGAAAATGGGTGGAAGCTATATGAC
CCTCTTTTAGAGTATAGAAGGCAGGGAATTCCAAATGAAAGCTGGAGAATAACAAAGATAAATGAACGAT
ATGAACTTTGTGATACATACCCTGCCCTCCTGGTTGTGCCAGCAAATATTCCTGATGAAGAATTAAAGAG
AGTGGCATCCTTCAGATCAAGAGGCCGTATCCCAGTTTTATCATGGATTCATCCTGAAAGTCAAGCCACA
ATCACTCGGTGTAGCCAGCCCATGGTTGGAGTGAGTGGAAAGCGAAGCAAAGAAGATGAAAAATACCTTC
AAGCTATCATGGATTCCAATGCCCAGTCTCACAAAATCTTTATATTTGATGCCCGGCCAAGTGTTAATGC
TGTTGCCAACAAGGCAAAGGGTGGAGGTTATGAAAGTGAAGATGCCTATCAAAATGCTGAACTAGTTTTC
CTGGATATCCACAATATTCATGTTATGAGAGAATCATTACGAAAACTTAAGGAGATTGTGTACCCCAACA
TTGAGGAAACTCACTGGTTGTCTAACTTGGAATCTACTCATTGGCTAGAACATATTAAGCTTATTCTTGC
AGGGGCTCTTAGGATTGCTGACAAGGTAGAGTCAGGGAAGACGTCTGTGGTAGTGCATTGCAGTGATGGT
TGGGATCGCACAGCTCAGCTCACTTCCCTTGCCATGCTCATGTTGGATGGATACTATCGAACCATCCGAG
GATTTGAAGTCCTTGTGGAGAAAGAATGGCTAAGTTTTGGACATCGATTTCAACTAAGAGTTGGCCATGG
AGATAAGAACCATGCAGATGCAGACAGATCGCCTGTTTTTCTTCAATTTATTGACTGTGTCTGGCAGATG
ACAAGACAGTTTCCTACCGCATTTGAATTCAATGAGTATTTTCTCATTACCATTTTGGACCACCTATACA
GCTGCTTATTCGGAACATTCCTCTGTAATAGTGAACAACAGAGAGGAAAAGAGAATCTTCCTAAAAGGAC
TGTGTCACTGTGGTCTTACATAAACAGCCAGCTGGAAGACTTCACTAATCCTCTCTATGGGAGCTATTCC
AATCATGTCCTTTATCCAGTAGCCAGCATGCGCCACCTAGAGCTCTGGGTGGGATATTACATAAGGTGGA
ATCCACGGATGAAACCACAGGAACCTATTCACAACAGATACAAAGAACTTCTTGCTAAACGAGCAGAGCT
TCAGAAAAAAGTAGAGGAACTACAGAGAGAGATTTCTAACCGATCAACCTCATCCTCAGAGAGAGCCAGC
TCTCCTGCACAGTGTGTCACTCCTGTCCAAACTGTTGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208703 protein sequence
Red=Cloning site Green=Tags(s)

METSSSCESLGSQPAAARPPSVDSLSSASTSHSENSVHTKSASVVSSDSISTSADNFSPDLRVLRESNKL
AEMEEPPLLPGENIKDMAKDVTYICPFTGAVRGTLTVTNYRLYFKSMERDPPFVLDASLGVINRVEKIGG
ASSRGENSYGLETVCKDIRNLRFAHKPEGRTRRSIFENLMKYAFPVSNNLPLFAFEYKEVFPENGWKLYD
PLLEYRRQGIPNESWRITKINERYELCDTYPALLVVPANIPDEELKRVASFRSRGRIPVLSWIHPESQAT
ITRCSQPMVGVSGKRSKEDEKYLQAIMDSNAQSHKIFIFDARPSVNAVANKAKGGGYESEDAYQNAELVF
LDIHNIHVMRESLRKLKEIVYPNIEETHWLSNLESTHWLEHIKLILAGALRIADKVESGKTSVVVHCSDG
WDRTAQLTSLAMLMLDGYYRTIRGFEVLVEKEWLSFGHRFQLRVGHGDKNHADADRSPVFLQFIDCVWQM
TRQFPTAFEFNEYFLITILDHLYSCLFGTFLCNSEQQRGKENLPKRTVSLWSYINSQLEDFTNPLYGSYS
NHVLYPVASMRHLELWVGYYIRWNPRMKPQEPIHNRYKELLAKRAELQKKVEELQREISNRSTSSSERAS
SPAQCVTPVQTVV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016156
ORF Size 1929 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016156.2
RefSeq Size 4603 bp
RefSeq ORF 1932 bp
Locus ID 8898
UniProt ID Q13614
Cytogenetics 11q21
Domains GRAM, PTPc_motif
Protein Families Druggable Genome, Phosphatase
Protein Pathways Fructose and mannose metabolism, Metabolic pathways, Riboflavin metabolism, Thiamine metabolism
MW 73.4 kDa
Summary This gene is a member of the myotubularin family of phosphoinositide lipid phosphatases. The encoded protein possesses phosphatase activity towards phosphatidylinositol-3-phosphate and phosphatidylinositol-3,5-bisphosphate. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4B, an autosomal recessive demyelinating neuropathy. Alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. provided by RefSeq, Aug 2011
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"NM_016156" in other vectors (6)
SKU Description Size Price
RC208703L1 Lenti ORF clone of Human myotubularin related protein 2 (MTMR2), transcript variant 1, Myc-DDK-tagged 10 ug
$898.00
RC208703L2 Lenti ORF clone of Human myotubularin related protein 2 (MTMR2), transcript variant 1, mGFP tagged 10 ug
$898.00
RC208703L3 Lenti ORF clone of Human myotubularin related protein 2 (MTMR2), transcript variant 1, Myc-DDK-tagged 10 ug
$898.00
RC208703L4 Lenti ORF clone of Human myotubularin related protein 2 (MTMR2), transcript variant 1, mGFP tagged 10 ug
$898.00
RG208703 MTMR2 (tGFP-tagged) - Human myotubularin related protein 2 (MTMR2), transcript variant 1 10 ug
$489.00 $798.00
SC127468 MTMR2 (untagged)-Human myotubularin related protein 2 (MTMR2), transcript variant 1 10 ug
$599.00

Plasmid Map for RC208703

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208703
Circular map for RC208703

file-alt Citations

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