SRC (NM_005417) Human Tagged ORF Clone
- TrueORF®
SKU
RC208622
SRC (Myc-DDK-tagged)-Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1
$289.00
$748.00
2 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SRC |
| Synonyms | ASV; c-SRC; p60-Src; SRC1; THC6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005417 |
| ORF Size | 1608 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005417.4 |
| RefSeq Size | 4145 bp |
| RefSeq ORF | 1611 bp |
| Locus ID | 6714 |
| UniProt ID | P12931 |
| Cytogenetics | 20q11.23 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Stem cell relevant signaling - JAK/STAT signaling pathway |
| Protein Pathways | Adherens junction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, GnRH signaling pathway, Tight junction, VEGF signaling pathway |
| MW | 59.7 kDa |
| Summary | This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005417" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208622L1 | Lenti ORF clone of Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,048.00
|
|
| RC208622L2 | Lenti ORF clone of Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1, mGFP tagged | 10 ug |
$1,048.00
|
|
| RC208622L3 | Lenti ORF clone of Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,048.00
|
|
| RC208622L4 | Lenti ORF clone of Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1, mGFP tagged | 10 ug |
$1,048.00
|
|
| RG208622 | SRC (tGFP-tagged) - Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1 | 10 ug |
$489.00
$948.00
|
|
| SC125208 | SRC (untagged)-Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1 | 10 ug |
$500.00
|
|
| SC323642 | SRC (untagged)-Kinase deficient mutant (K298M) of Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1 | 10 ug |
$500.00
|
|
Citations
Lusianthridin targeting of lung cancer stem cells via Src-STAT3 suppression
Bhummaphan, Petpiroon, Prakhongcheep et al
Phytomedicine (2019) 62, 152932 DOI: 10.1016/j.phymed.2019.152932
Bhummaphan, Petpiroon, Prakhongcheep et al
Phytomedicine (2019) 62, 152932 DOI: 10.1016/j.phymed.2019.152932
Cited by 57
AI Summary: In this study, OriGene products—specifically the Src (RC217839) expression plasmid and shRNA-Src (TL313386)—were used to manipulate Src levels in lung cancer cells, enabling the researchers to demonstrate that lusianthridin suppresses cancer stem cell phenotypes via the Src-STAT3 pathway.
EGF regulates the interaction of Tks4 with Src through its SH2 and SH3 domains
Dulk, Szeder, Glatz et al
Biochemistry (2018)
Dulk, Szeder, Glatz et al
Biochemistry (2018)
AI Summary: OriGene's pCMV6 mammalian expression vector containing the full-length human Src gene was used in this study to investigate the molecular interaction between Tks4 and Src in EGF signaling.
Snippet: expression vector pcDNA3.1/TOPO-V5-His was described previously11. The mammalian expression vector pCMV6 harboring the full-length coding region of human Src (UniProt ID P12931) was purchased from OriGene Technologies, Inc. These plasmids were used
Androgen enhances the activity of ETS-1 and promotes the proliferation of HCC cells
Ren, Ren, Zhang et al
Oncotarget (2017) 8 (65), 109271-109288 DOI: 10.18632/oncotarget.22669
Ren, Ren, Zhang et al
Oncotarget (2017) 8 (65), 109271-109288 DOI: 10.18632/oncotarget.22669
Snippet: The expression vectors of ETS-1, FLAG-ETS1, AR, FLAG-AR, AR siRNA, ARE-Luc, MMP1-Luc, and MMP9-Luc were gifts from Dr. Jiajun Cui and Dr. Fan Feng as described in references [2] and [3]. The ETS-1 point mutation sequence was obtained by using chemical synthesis methods and then cloned into a pcDNA3.1 plasmid. The expression vectors of SRC-1 and AIB-1 were purchased from Origene Company, USA
CDK dependent phosphorylation of PHD1 on Serine 130 determines specificity in substrate targeting in cells
Ortmann, Bensaddek, Carvalhal et al
J. Cell. Sci. (2015)
Ortmann, Bensaddek, Carvalhal et al
J. Cell. Sci. (2015)
Cited by 19
AI Summary: OriGene products contributed to this study by providing the CMV-HIF1α and CMV-Src expression constructs, which were used to investigate the molecular mechanisms underlying PHD1 phosphorylation and its effects on substrate specificity.
Snippet: as templates with the primers described in Sup. Information. CMV-HIF1α and CMV-Src expression constructs were obtained from Origene. E2F1-ER and c-Myc widtype and mutant expression constructs were a kind gift from Dr. Victoria Cowling (University of Dundee ... Cite SaveSaving
Showing 1-4 of 4 papers.
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