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SRC (NM_005417) Human Untagged Clone

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SKU
SC125208
SRC (untagged)-Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1
  $500.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SRC
Synonyms ASV; c-SRC; p60-Src; SRC1; THC6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC125208 sequence for NM_005417 edited (data generated by NextGen Sequencing)
ATGGGTAGCAACAAGAGCAAGCCCAAGGATGCCAGCCAGCGGCGCCGCAGCCTGGAGCCC
GCCGAGAACGTGCACGGCGCTGGCGGGGGCGCTTTCCCCGCCTCGCAGACCCCCAGCAAG
CCAGCCTCGGCCGACGGCCACCGCGGCCCCAGCGCGGCCTTCGCCCCCGCGGCCGCCGAG
CCCAAGCTGTTCGGAGGCTTCAACTCCTCGGACACCGTCACCTCCCCGCAGAGGGCGGGC
CCGCTGGCCGGTGGAGTGACCACCTTTGTGGCCCTCTATGACTATGAGTCTAGGACGGAG
ACAGACCTGTCCTTCAAGAAAGGCGAGCGGCTCCAGATTGTCAACAACACAGAGGGAGAC
TGGTGGCTGGCCCACTCGCTCAGCACAGGACAGACAGGCTACATCCCCAGCAACTACGTG
GCGCCCTCCGACTCCATCCAGGCTGAGGAGTGGTATTTTGGCAAGATCACCAGACGGGAG
TCAGAGCGGTTACTGCTCAATGCAGAGAACCCGAGAGGGACCTTCCTCGTGCGAGAAAGT
GAGACCACGAAAGGTGCCTACTGCCTCTCAGTGTCTGACTTCGACAACGCCAAGGGCCTC
AACGTGAAGCACTACAAGATCCGCAAGCTGGACAGCGGCGGCTTCTACATCACCTCCCGC
ACCCAGTTCAACAGCCTGCAGCAGCTGGTGGCCTACTACTCCAAACACGCCGATGGCCTG
TGCCACCGCCTCACCACCGTGTGCCCCACGTCCAAGCCGCAGACTCAGGGCCTGGCCAAG
GATGCCTGGGAGATCCCTCGGGAGTCGCTGCGGCTGGAGGTCAAGCTGGGCCAGGGCTGC
TTTGGCGAGGTGTGGATGGGGACCTGGAACGGTACCACCAGGGTGGCCATCAAAACCCTG
AAGCCTGGCACGATGTCTCCAGAGGCCTTCCTGCAGGAGGCCCAGGTCATGAAGAAGCTG
AGGCATGAGAAGCTGGTGCAGTTGTATGCTGTGGTTTCAGAGGAGCCCATTTACATCGTC
ACGGAGTACATGAGCAAGGGGAGTTTGCTGGACTTTCTCAAGGGGGAGACAGGCAAGTAC
CTGCGGCTGCCTCAGCTGGTGGACATGGCTGCTCAGATCGCCTCAGGCATGGCGTACGTG
GAGCGGATGAACTACGTCCACCGGGACCTTCGTGCAGCCAACATCCTGGTGGGAGAGAAC
CTGGTGTGCAAAGTGGCCGACTTTGGGCTGGCTCGGCTCATTGAAGACAATGAGTACACG
GCGCGGCAAGGTGCCAAATTCCCCATCAAGTGGACGGCTCCAGAAGCTGCCCTCTATGGC
CGCTTCACCATCAAGTCGGACGTGTGGTCCTTCGGGATCCTGCTGACTGAGCTCACCACA
AAGGGACGGGTGCCCTACCCTGGGATGGTGAACCGCGAGGTGCTGGACCAGGTGGAGCGG
GGCTACCGGATGCCCTGCCCGCCGGAGTGTCCCGAGTCCCTGCACGACCTCATGTGCCAG
TGCTGGCGGAAGGAGCCTGAGGAGCGGCCCACCTTCGAGTACCTGCAGGCCTTCCTGGAG
GACTACTTCACGTCCACCGAGCCCCAGTACCAGCCCGGGGAGAACCTCTAG

Clone variation with respect to NM_005417.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005417 unedited
NAGTTCGCAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCCGGA
CGGCCAGGCCGCCGTCTGCCCGTCCCGCTGGACGTCCCGCGGTCCGCCCTCCCGTGCGTC
CGTCTGCCGGTGAGCCCGCCCGCCCGCCGGCCCAGAACAGAGAACAGAAGCTCAGAGAAG
TGAAGCAACTTGCCCAGCTATGAGAGACAGAGCCAGGATTTGAAACCAGATGAGGACGCT
GAGGCCCAGAGAGGGAAAGCCACTTGCCTAGGGACACACAGCGGGGAGAGGTGGAGCAGG
GCCTCTATTTCGAGACCCCTGACTCCACACCTGGTGTTTGTGCCAAGACCCCAGGCTGCC
TCCCAGGTCCTCTGGGACAGCCCCTGCCTTCTACCAGGACCATGGGTAGCAACAAGAGCA
AGCCCAAGGATGCCAGCCAGCGGCGCCGCAGCCTGGAGCCCGCCGAGAACGTGCACGGCG
CTGGCGGGGGCGCTTTCCCCGCCTCGCAGACCCCCAGCAAGCCAGCCTCGGCCGACGGCC
ACCGCGGCCCCAGCGCGGCCTTCGCCCCCGCGGCCGCCGAGCCCAAGCTGTTCGGAGGCT
TCAACTCCTCGGACACCGTCACCTCCCCGCAGAGGGCGGGCCCGCTGGCCGGTGGAGTGA
CCACCTTTGTGGCCCTCTATGACTATGAGTCTAGGACGGAGACAGACCTGTCCTTCAAGA
AGGCGAGCGGGCTCCGATTGTCAACAACACAAAGGGAGACTGGTGGCTGGCCCACTCGCT
CAGCACAGGACAGACAGGCTACATTCCCAGCAACTAAGTGGCGCCCTTCGACTCCTTCCA
GGCTGAAGGATTGGTATTTTGGGCAGATCACCAGACGGGGAGTG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005417 unedited
NANTTGCACTGGGGAGGCGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTAT
GACCGTGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTCTGAGGTCTGTTTGT
GTTTAATCAGACAATATGCAAAGTATTTACAACCAATTCTGGATACCCCCCCACCCCCCG
CAAGTCTGGGCCTTGGAATTTCGGAGCCCCAGGCCCGGCCCAGAGCCAGGGGTCCCCCAG
GCCTCTGCATAGTCATCTGAAATCTACAAAACACTGTTAAAAAAAAAAAGGACAGTATTA
AGACACCTTACACAAAGGGCCTTATGCAGTTGGAGAGGGATTTGAGAGCTCGCTGGGGTG
AGTGAGCCCAGATTTGACTGGAATGGACGGGAGAAGACTTGGGTAATAAAGACGTATGAA
AGGAAAAGTTGAAATTTCATAGGCAGGCACTGCTTGGCCTCCTTCCCCACTGGCAGGGCC
TTGCTGGCTTCAAGAACCCCTGCAGTGGGAATGTTATTGCTATGATGAGGGGNGCCATGG
TGAATGTACAACAATTGTTGGNGGACGNGAGCGNGGGGACATTNTTACATGTTAAATATT
GGACGCACATGGAGACACGCACACATGCACACACATACACACACACAGGGACCCTTTGCA
AGATGCCACNACTTGTAGCTTGCCTTTTACAGATGGGCACACTGGGCCCAGGGAGATCAA
CACACAACCAAAGGCCGACAGAGCANAAGCCNATTTNCTTGATGTCAAGCGTTGNCTTTC
ATCTTTGCTGTCCAAG
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_005417
Insert Size 1611 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation no
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005417.3
RefSeq Size 4131 bp
RefSeq ORF 1611 bp
Locus ID 6714
UniProt ID P12931
Cytogenetics 20q11.23
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Stem cell relevant signaling - JAK/STAT signaling pathway
Protein Pathways Adherens junction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, GnRH signaling pathway, Tight junction, VEGF signaling pathway
MW 59.8 kDa
Summary This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.
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Documents
Resources
"NM_005417" in other vectors (7)
SKU Description Size Price
RC208622 SRC (Myc-DDK-tagged)-Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1 10 ug
$289.00 $748.00
RC208622L1 Lenti ORF clone of Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1, Myc-DDK-tagged 10 ug
$1,048.00
RC208622L2 Lenti ORF clone of Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1, mGFP tagged 10 ug
$1,048.00
RC208622L3 Lenti ORF clone of Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1, Myc-DDK-tagged 10 ug
$1,048.00
RC208622L4 Lenti ORF clone of Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1, mGFP tagged 10 ug
$1,048.00
RG208622 SRC (tGFP-tagged) - Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1 10 ug
$489.00 $948.00
SC323642 SRC (untagged)-Kinase deficient mutant (K298M) of Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1 10 ug
$500.00

file-alt Citations

A Dimerization Function in the Intrinsically Disordered N-Terminal Region of Src
Spassov, Ruiz-Saenz, Piple et al
Cell Rep (2018) 25 (2), 449-463.e4 DOI: 10.1016/j.celrep.2018.09.035
AI Summary: OriGene’s pCMV6-cSRC plasmid was used in this study to investigate the role of Src’s N-terminal region in kinase dimerization and its effects on autophosphorylation and substrate phosphorylation.
Snippet: pDEST40-2XHA-nSNAP Modified from pDEST40 N/A pDEST40-2XFLAG-cSNAP Modified from pDEST40 N/A pCMV6-cSRC Origene sc12
All-trans-retinoic acid activates the pro-invasive Src-YAP-Interleukin 6 axis in triple-negative MDA-MB-231 breast cancer cells while cerivastatin reverses this action
Mezquita, Mezquita, Pau et al
Sci Rep (2018) 8 (1), 7047 DOI: 10.1038/s41598-018-25526-1
AI Summary: OriGene contributed to this study by providing the Src plasmid (sc125208), which was used to investigate the role of Src in the pro-invasive signaling pathway activated by all-trans-retinoic acid in breast cancer cells.
Snippet: Transfection of Src Transfections were carried using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions. Src plasmid was from Origene (sc125208). Interference of Src Interference of Src was accomplished using a pre-designed ON-TARGET SMART pool from ThermoScientific (L-003175). …
FigureFigureFigure
Frank-ter Haar syndrome protein Tks4 regulates epidermal growth factor-dependent cell migration
Bögel, Gujdár, Geiszt et al
J. Biol. Chem. (2012) 287 (37), 31321-9 DOI: 10.1074/jbc.M111.324897
Cited by 1
AI Summary: OriGene’s pCMV6-Src mammalian expression vector was used to provide full-length Src cDNA, enabling the study of Src’s role in Tks4 phosphorylation and EGF-dependent cell migration in this research.
Snippet: using the QuikChange Site-directed Mutagenesis Kit (Stratagene). cDNA of full-length Src in mammalian expression vector pCMV6 was purchased from OriGene Technologies, Inc. Chicken ΔSH2 Src and ΔSH3 Src constructs
Src tyrosine kinase phosphorylation of nuclear receptor HNF4α correlates with isoform-specific loss of HNF4α in human colon cancer
Chellappa, Jankova, Schnabl et al
Proc Natl Acad Sci U S A (2012) 109 (7), 2302-7 DOI: 10.1073/pnas.1106799109
Cited by 86
AI Summary: OriGene products, specifically the HNF4A cDNA clone (RC211919) and HNF4A antibody (TA500494), were used in this study to investigate the phosphorylation and expression of HNF4α by Src kinase in colon cancer cells.
Showing 1-4 of 4 papers.
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