PARVB (NM_013327) Human Tagged ORF Clone

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RC208490
PARVB (Myc-DDK-tagged)-Human parvin, beta (PARVB), transcript variant 2
  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PARVB
Synonyms CGI-56
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208490 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTCCGCGCCGCGCTCGCCCACCCCGCGGCCCCGCAGGATGAAGAAGGACGAGTCGTTCCTGGGCA
AGCTGGGCGGCACCCTGGCCAGGAAGCGGAGGGCGCGCGAGGTGAGTGACCTGCAGGAAGAAGGCAAGAA
TGCCATCAACTCACCGATGTCCCCCGCCCTGGCGGATGTTCACCCTGAAGACACCCAGCTCGAGGAGAAC
GAGGAGCGCACGATGATTGACCCCACTTCCAAGGAAGACCCCAAGTTCAAGGAACTGGTCAAGGTCCTCC
TCGACTGGATTAATGACGTGCTGGTGGAGGAGAGGATCATTGTGAAGCAGCTGGAGGAAGACCTGTATGA
CGGCCAGGTGCTGCAGAAGCTCTTGGAAAAACTGGCAGGGTGCAAGCTGAATGTGGCTGAGGTGACACAG
TCCGAAATAGGGCAGAAACAGAAGCTGCAGACGGTGCTGGAAGCAGTACATGACCTGCTGCGGCCCCGAG
GCTGGGCGCTCCGGTGGAGCGTGGACTCAATTCACGGGAAGAACCTGGTGGCCATCCTCCACCTGCTGGT
CTCTCTGGCCATGCACTTCAGGGCCCCCATCCGCCTTCCTGAGCATGTAACGGTGCAGGTGGTGGTCGTG
CGGAAACGGGAAGGCCTGCTGCATTCCAGCCACATCTCGGAGGAGCTGACCACAACTACAGAGATGATGA
TGGGCCGGTTCGAGCGGGATGCCTTCGACACGCTGTTCGACCACGCCCCGGATAAGCTCAGCGTGGTGAA
GAAGTCTCTCATCACTTTTGTGAACAAGCACCTGAACAAGCTGAATTTGGAGGTGACGGAACTGGAGACC
CAGTTTGCAGATGGCGTGTACCTGGTTCTGCTCATGGGCCTTCTGGAAGACTACTTTGTTCCTCTCCACC
ACTTCTACCTGACTCCGGAAAGCTTCGATCAGAAGGTCCACAATGTGTCCTTTGCCTTTGAGCTGATGCT
GGACGGAGGCCTCAAGAAACCCAAGGCTCGTCCTGAAGACGTGGTTAACTTGGACCTCAAATCCACCCTG
AGGGTTCTTTACAACCTGTTCACCAAGTACAAGAACGTGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC208490 protein sequence
Red=Cloning site Green=Tags(s)

MSSAPRSPTPRPRRMKKDESFLGKLGGTLARKRRAREVSDLQEEGKNAINSPMSPALADVHPEDTQLEEN
EERTMIDPTSKEDPKFKELVKVLLDWINDVLVEERIIVKQLEEDLYDGQVLQKLLEKLAGCKLNVAEVTQ
SEIGQKQKLQTVLEAVHDLLRPRGWALRWSVDSIHGKNLVAILHLLVSLAMHFRAPIRLPEHVTVQVVVV
RKREGLLHSSHISEELTTTTEMMMGRFERDAFDTLFDHAPDKLSVVKKSLITFVNKHLNKLNLEVTELET
QFADGVYLVLLMGLLEDYFVPLHHFYLTPESFDQKVHNVSFAFELMLDGGLKKPKARPEDVVNLDLKSTL
RVLYNLFTKYKNVE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013327
ORF Size 1092 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013327.5
RefSeq Size 1725 bp
RefSeq ORF 1095 bp
Locus ID 29780
UniProt ID Q9HBI1
Cytogenetics 22q13.31
Domains CH
Protein Pathways Focal adhesion
MW 41.7 kDa
Summary This gene encodes a member of the parvin family of actin-binding proteins, which play a role in cytoskeleton organization and cell adhesion. These proteins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. This family member binds to alphaPIX and alpha-actinin, and it can inhibit the activity of integrin-linked kinase. This protein also functions in tumor suppression. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Aug 2011
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Documents
Resources
"NM_013327" in other vectors (4)
SKU Description Size Price
RC208490L3 Lenti ORF clone of Human parvin, beta (PARVB), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC208490L4 Lenti ORF clone of Human parvin, beta (PARVB), transcript variant 2, mGFP tagged 10 ug
$757.00
RG208490 PARVB (tGFP-tagged) - Human parvin, beta (PARVB), transcript variant 2 10 ug
$489.00 $657.00
SC324009 PARVB (untagged)-Human parvin, beta (PARVB), transcript variant 2 10 ug
$457.00

Plasmid Map for RC208490

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Cloning scheme for RC208490

Circular map for RC208490

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