PARVB (NM_013327) Human Untagged Clone

SKU SC324009

PARVB (untagged)-Human parvin, beta (PARVB), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: PARVB
• Synonyms: CGI-56
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_013327.3 TGCGCCCGCCGCCGGCCCCACGCGCGGCCCATGTCCTCCGCGCCGCGCTCGCCCACCCCG; CGGCCCCGCAGGATGAAGAAGGACGAGTCGTTCCTGGGCAAGCTGGGCGGCACCCTGGCC; AGGAAGCGGAGGGCGCGCGAGGTGAGTGACCTGCAGGAAGAAGGCAAGAATGCCATCAAC; TCACCGATGTCCCCCGCCCTGGCGGATGTTCACCCTGAAGACACCCAGCTCGAGGAGAAC; GAGGAGCGCACGATGATTGACCCCACTTCCAAGGAAGACCCCAAGTTCAAGGAACTGGTC; AAGGTCCTCCTCGACTGGATTAATGACGTGCTGGTGGAGGAGAGGATCATTGTGAAGCAG; CTGGAGGAAGACCTGTATGACGGCCAGGTGCTGCAGAAGCTCTTGGAAAAACTGGCAGGG; TGCAAGCTGAATGTGGCTGAGGTGACACAGTCCGAAATAGGGCAGAAACAGAAGCTGCAG; ACGGTGCTGGAAGCAGTACATGACCTGCTGCGGCCCCGAGGCTGGGCGCTCCGGTGGAGC; GTGGACTCAATTCACGGGAAGAACCTGGTGGCCATCCTCCACCTGCTGGTCTCTCTGGCC; ATGCACTTCAGGGCCCCCATCCGCCTTCCTGAGCATGTAACGGTGCAGGTGGTGGTCGTG; CGGAAACGGGAAGGCCTGCTGCATTCCAGCCACATCTCGGAGGAGCTGACCACAACTACA; GAGATGATGATGGGCCGGTTCGAGCGGGATGCCTTCGACACGCTGTTCGACCACGCCCCG; GATAAGCTCAGCGTGGTGAAGAAGTCTCTCATCACTTTTGTGAACAAGCACCTGAACAAG; CTGAATTTGGAGGTGACGGAACTGGAGACCCAGTTTGCAGATGGCGTGTACCTGGTTCTG; CTCATGGGCCTTCTGGAAGACTACTTTGTTCCTCTCCACCACTTCTACCTGACTCCGGAA; AGCTTCGATCAGAAGGTCCACAATGTGTCCTTTGCCTTTGAGCTGATGCTGGACGGAGGC; CTCAAGAAACCCAAGGCTCGTCCTGAAGACGTGGTTAACTTGGACCTCAAATCCACCCTG; AGGGTTCTTTACAACCTGTTCACCAAGTACAAGAACGTGGAGTGACGGGGGAGCTGTGGA; TGGTGGCAGGAGTGTCCCAGCAAGAAAGGCGGCATCCGTCTGTGCCCTGTGCCTTTCCAG; GGAGCCAGGCGCCATGGGCTTCTGGTCCAAGCTGTGTTGACTGTCATCCCCACCCCACCC; CTACCTCACGCCTGCCCCACCCCCTGCCTCTTTTGGTTGTTGTTCTTAATCTCCTCTCCA; TGTAGTTCCCAGTGGGCAAGAGCCTTTGAAAATGCAGGATTCTAAACACTCGTGCTTGCG; TTTGAAGCCTCGCGTCACTCAGTCGCGTGGGATGATGAGTCCGTTGTTGTCTGCCTTGGC; CGAAAGATGAAAAAAAGCCTGAACCCCAACCCCCAGCTGGTTGAGAGCACCCTGCATTCT; GCCTCATGGTGCAGTTAGCGATCACAGGCCTTCAGAAGTACAACATCAGCTCAGCAGGAA; CGCCGGCTCCCCGAGGACCCAGGCTTGACGATTCACCGGGGATCTCCTGGGCTGGGCTCT; CCTTGGAAGTGAGGCCTTTTATTAAAAATAAAAGGGTTTTGCAGTTTGAAAAAAAAAAAA; AAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_013327
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013327.3, NP_037459.2
• RefSeq Size: 1713 bp
• RefSeq ORF: 1095 bp
• Locus ID: 29780
• UniProt ID: Q9HBI1
• Cytogenetics: 22q13.31
• Domains: CH
• Protein Pathways: Focal adhesion
• Summary: This gene encodes a member of the parvin family of actin-binding proteins, which play a role in cytoskeleton organization and cell adhesion. These proteins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. This family member binds to alphaPIX and alpha-actinin, and it can inhibit the activity of integrin-linked kinase. This protein also functions in tumor suppression. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]; Transcript Variant: This variant (2, also known as ParvB2) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The resulting isoform (b) has a distinct and shorter N-terminus, compared to isoform a.