ECEL1 (NM_004826) Human Tagged ORF Clone

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SKU
RC208405
ECEL1 (Myc-DDK-tagged)-Human endothelin converting enzyme-like 1 (ECEL1)
  $1,064.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ECEL1
Synonyms DA5D; DINE; ECEX; XCE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208405 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCCCGTATTCGCTGACGGCGCACTACGATGAGTTCCAAGAGGTCAAGTACGTGAGCCGCTGCG
GCGCGGGGGGCGCGCGCGGGGCCTCCCTGCCCCCGGGCTTCCCGTTGGGCGCTGCGCGCAGCGCCACCGG
GGCCCGGTCCGGGCTGCCGCGCTGGAACCGGCGCGAGGTGTGCCTGCTGTCGGGGCTGGTGTTCGCCGCC
GGCCTCTGCGCCATTCTGGCGGCTATGCTGGCCCTCAAGTACCTGGGCCCGGTCGCGGCCGGCGGCGGCG
CCTGTCCCGAGGGCTGCCCTGAGCGCAAGGCCTTCGCGCGCGCCGCTCGCTTCCTGGCCGCCAACCTGGA
CGCCAGCATCGACCCATGCCAGGACTTCTACTCGTTCGCCTGCGGCGGTTGGCTGCGGCGCCACGCCATC
CCCGACGACAAGCTCACCTATGGCACCATCGCGGCCATCGGCGAGCAAAACGAGGAGCGCCTACGGCGCC
TGCTGGCGCGGCCCGGGGGTGGGCCTGGCGGCGCGGCCCAGCGCAAGGTGCGCGCCTTCTTCCGCTCGTG
CCTCGACATGCGCGAGATCGAGCGACTGGGCCCGCGACCCATGCTAGAGGTCATCGAGGACTGCGGGGGC
TGGGACCTGGGCGGCGCGGAGGAGCGTCCGGGGGTCGCGGCGCGATGGGACCTCAACCGGCTGCTGTACA
AGGCGCAGGGCGTGTACAGCGCCGCCGCGCTCTTCTCGCTCACGGTCAGCCTGGACGACAGGAACTCCTC
GCGCTACGTCATCCGCATTGACCAGGATGGGCTCACCCTGCCAGAGAGGACCCTGTACCTCGCTCAGGAT
GAGGACAGTGAGAAGATCCTGGCAGCATACAGGGTGTTCATGGAGCGAGTGCTCAGCCTCCTGGGTGCAG
ACGCTGTGGAACAGAAGGCCCAAGAGATCCTGCAAGTGGAGCAGCAGCTGGCCAACATCACTGTGTCAGA
GTATGACGACCTACGGCGAGATGTCAGCTCCATGTACAACAAGGTGACGCTGGGGCAGCTGCAGAAGATC
ACCCCCCACTTGCGGTGGAAGTGGCTGCTAGACCAGATCTTCCAGGAGGACTTCTCAGAGGAAGAGGAGG
TGGTGCTGCTGGCGACAGACTACATGCAGCAGGTGTCGCAGCTCATCCGCTCCACACCCCACCGGGTCCT
GCACAACTACCTGGTGTGGCGCGTGGTGGTGGTCCTGAGTGAACACCTGTCCCCGCCATTCCGTGAGGCA
CTGCACGAGCTGGCACAGGAGATGGAGGGCAGCGACAAGCCACAGGAGCTGGCCCGGGTCTGCTTGGGCC
AGGCCAATCGCCACTTTGGCATGGCGCTTGGCGCCCTCTTTGTACATGAGCACTTCTCAGCTGCCAGCAA
AGCCAAGGTGCAGCAGCTAGTGGAAGACATCAAGTACATCCTGGGCCAGCGCCTGGAGGAGCTGGACTGG
ATGGACGCCGAGACCAGGGCTGCTGCTCGGGCCAAGCTCCAGTACATGATGGTGATGGTCGGCTACCCGG
ACTTCCTGCTGAAACCCGATGCTGTGGACAAGGAGTATGAGTTTGAGGTCCATGAGAAGACCTACTTCAA
GAACATCTTGAACAGCATCCGCTTCAGCATCCAGCTCTCAGTTAAGAAGATTCGGCAGGAGGTGGACAAG
TCCACGTGGCTGCTCCCCCCACAGGCGCTCAATGCCTACTATCTACCCAACAAGAACCAGATGGTGTTCC
CCGCGGGCATCCTGCAGCCCACCCTGTACGACCCTGACTTCCCACAGTCTCTCAACTACGGGGGCATCGG
CACCATCATTGGACATGAGCTGACCCACGGCTACGACGACTGGGGGGGCCAGTATGACCGCTCAGGGAAC
CTGCTGCACTGGTGGACGGAGGCCTCCTACAGCCGCTTCCTGCGAAAGGCTGAGTGCATCGTCCGTCTCT
ATGACAACTTCACTGTCTACAACCAGCGGGTGAACGGGAAACACACGCTTGGGGAGAACATCGCAGATAT
GGGCGGCCTCAAGCTGGCCTACCACGCCTATCAGAAGTGGGTGCGGGAGCACGGCCCAGAGCACCCACTT
CCCCGGCTCAAGTACACACATGACCAGCTCTTCTTCATTGCCTTTGCCCAGAACTGGTGCATCAAGCGGC
GGTCGCAGTCCATCTACCTGCAGGTGCTGACTGACAAGCATGCCCCTGAGCACTACAGGGTGCTGGGCAG
TGTGTCCCAGTTTGAGGAGTTTGGCCGGGCTTTCCACTGTCCCAAGGACTCACCCATGAACCCTGCCCAC
AAGTGTTCCGTGTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC208405 protein sequence
Red=Cloning site Green=Tags(s)

MEPPYSLTAHYDEFQEVKYVSRCGAGGARGASLPPGFPLGAARSATGARSGLPRWNRREVCLLSGLVFAA
GLCAILAAMLALKYLGPVAAGGGACPEGCPERKAFARAARFLAANLDASIDPCQDFYSFACGGWLRRHAI
PDDKLTYGTIAAIGEQNEERLRRLLARPGGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGG
WDLGGAEERPGVAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQD
EDSEKILAAYRVFMERVLSLLGADAVEQKAQEILQVEQQLANITVSEYDDLRRDVSSMYNKVTLGQLQKI
TPHLRWKWLLDQIFQEDFSEEEEVVLLATDYMQQVSQLIRSTPHRVLHNYLVWRVVVVLSEHLSPPFREA
LHELAQEMEGSDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKYILGQRLEELDW
MDAETRAAARAKLQYMMVMVGYPDFLLKPDAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDK
STWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGN
LLHWWTEASYSRFLRKAECIVRLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYHAYQKWVREHGPEHPL
PRLKYTHDQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPAH
KCSVW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004826
ORF Size 2325 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004826.4
RefSeq Size 2909 bp
RefSeq ORF 2328 bp
Locus ID 9427
UniProt ID O95672
Cytogenetics 2q37.1
Protein Families Druggable Genome, Protease, Transmembrane
MW 87.8 kDa
Summary This gene encodes a member of the M13 family of endopeptidases. Members of this family are zinc-containing type II integral-membrane proteins that are important regulators of neuropeptide and peptide hormone activity. Mutations in this gene are associated with autosomal recessive distal arthrogryposis, type 5D. This gene has multiple pseudogenes on chromosome 2. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Mar 2014
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Documents
Resources
"NM_004826" in other vectors (4)
SKU Description Size Price
RC208405L3 Lenti ORF clone of Human endothelin converting enzyme-like 1 (ECEL1), Myc-DDK-tagged 10 ug
$1,364.00
RC208405L4 Lenti ORF clone of Human endothelin converting enzyme-like 1 (ECEL1), mGFP tagged 10 ug
$1,364.00
RG208405 ECEL1 (tGFP-tagged) - Human endothelin converting enzyme-like 1 (ECEL1) 10 ug
$1,264.00
SC125561 ECEL1 (untagged)-Human endothelin converting enzyme-like 1 (ECEL1) 10 ug
$1,066.00

Plasmid Map for RC208405

Download sequence for

Cloning scheme for RC208405

Circular map for RC208405

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