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ECEL1 (NM_004826) Human Untagged Clone

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SKU
SC125561
ECEL1 (untagged)-Human endothelin converting enzyme-like 1 (ECEL1)
  $1,066.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ECEL1
Synonyms DA5D; DINE; ECEX; XCE
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_004826 edited
GGCGGCGGGCGCTGGGAGACACCGGACGCCCGCTCGGCTGCGCTGCGGCTCAGGCCCCCG
CTCGGGCCCGACCCGCTCGGTCACCGCCGGCTCGGGCGCGCACCTGCCGGCTGCGGCCCC
AGGGCCATGCGGAGGCCCACGAGGAGGCCGGCGGCCACGCGCATCCCGTAGCCCAGGTGG
CCCAGGTCTGCACCGCGGCGGCCTCGGCGCCATGGAGCCCCCGTATTCGCTGACGGCGCA
CTACGATGAGTTCCAAGAGGTCAAGTACGTGAGCCGCTGCGGCGCGGGGGGCGCGCGCGG
GGCCTCCCTGCCCCCGGGCTTCCCGTTGGGCGCTGCGCGCAGCGCCACCGGGGCCCGGTC
CGGGCTGCCGCGCTGGAACCGGCGCGAGGTGTGCCTGCTGTCGGGGCTGGTGTTCGCCGC
CGGCCTCTGCGCCATTCTGGCGGCTATGCTGGCCCTCAAGTACCTGGGCCCGGTCGCGGC
CGGCGGCGGCGCCTGTCCCGAGGGCTGCCCTGAGCGCAAGGCCTTCGCGCGCGCCGCTCG
CTTCCTGGCCGCCAACCTGGACGCCAGCATCGACCCATGCCAGGACTTCTACTCGTTCGC
CTGCGGCGGTTGGCTGCGGCGCCACGCCATCCCCGACGACAAGCTCACCTATGGCACCAT
CGCGGCCATCGGCGAGCAAAACGAGGAGCGCCTACGGCGCCTGCTGGCGCGGCCCGGGGG
TGGGCCTGGCGGCGCGGCCCAGCGCAAGGTGCGCGCCTTCTTCCGCTCGTGCCTCGACAT
GCGCGAGATCGAGCGACTGGGCCCGCGACCCATGCTAGAGGTCATCGAGGACTGCGGGGG
CTGGGACCTGGGCGGCGCGGAGGAGCGTCCGGGGGTCGCGGCGCGATGGGACCTCAACCG
GCTGCTGTACAAGGCGCAGGGCGTGTACAGCGCCGCCGCGCTCTTCTCGCTCACGGTCAG
CCTGGACGACAGGAACTCCTCGCGCTACGTCATCCGCATTGACCAGGATGGGCTCACCCT
GCCAGAGAGGACCCTGTACCTCGCTCAGGATGAGGACAGTGAGAAGATCCTGGCAGCATA
CAGGGTGTTCATGGAGCGAGTGCTCAGCCTCCTGGGTGCAGACGCTGTGGAACAGAAGGC
CCAAGAGATCCTGCAAGTGGAGCAGCAGCTGGCCAACATCACTGTGTCAGAGTATGACGA
CCTACGGCGAGATGTCAGCTCCATGTACAACAAGGTGACGCTGGGGCAGCTGCAGAAGAT
CACCCCCCACTTGCGGTGGAAGTGGCTGCTAGACCAGATCTTCCAGGAGGACTTCTCAGA
GGAAGAGGAGGTGGTGCTGCTGGCGACAGACTACATGCAGCAGGTGTCGCAGCTCATCCG
CTCCACACCCCACCGGGTCCTGCACAACTACCTGGTGTGGCGCGTGGTGGTGGTCCTGAG
TGAACACCTGTCCCCGCCATTCCGTGAGGCACTGCACGAGCTGGCACAGGAGATGGAGGG
CAGCGACAAGCCACAGGAGCTGGCCCGGGTCTGCTTGGGCCAGGCCAATCGCCACTTTGG
CATGGCGCTTGGCGCCCTCTTTGTACATGAGCACTTCTCAGCTGCCAGCAAAGCCAAGGT
GCAGCAGCTAGTGGAAGACATCAAGTACATCCTGGGCCAGCGCCTGGAGGAGCTGGACTG
GATGGACGCCGAGACCAGGGCTGCTGCTCGGGCCAAGCTCCAGTACATGATGGTGATGGT
CGGCTACCCGGACTTCCTGCTGAAACCCGATGCTGTGGACAAGGAGTATGAGTTTGAGGT
CCATGAGAAGACCTACTTCAAGAACATCTTGAACAGCATCCGCTTCAGCATCCAGCTCTC
AGTTAAGAAGATTCGGCAGGAGGTGGACAAGTCCACGTGGCTGCTCCCCCCACAGGCGCT
CAATGCCTACTATCTACCCAACAAGAACCAGATGGTGTTCCCCGCGGGCATCCTGCAGCC
CACCCTGTACGACCCTGACTTCCCACAGTCTCTCAACTACGGGGGCATCGGCACCATCAT
TGGACATGAGCTGACCCACGGCTACGACGACTGGGGGGGCCAGTATGACCGCTCAGGGAA
CCTGCTGCACTGGTGGACGGAGGCCTCCTACAGCCGCTTCCTGCGAAAGGCTGAGTGCAT
CGTCCGTCTCTATGACAACTTCACTGTCTACAACCAGCGGGTGAACGGGAAACACACGCT
TGGGGAGAACATCGCAGATATGGGCGGCCTCAAGCTGGCCTACCACGCCTATCAGAAGTG
GGTGCGGGAGCACGGCCCAGAGCACCCACTTCCCCGGCTCAAGTACACACATGACCAGCT
CTTCTTCATTGCCTTTGCCCAGAACTGGTGCATCAAGCGGCGGTCGCAGTCCATCTACCT
GCAGGTGCTGACTGACAAGCATGCCCCTGAGCACTACAGGGTGCTGGGCAGTGTGTCCCA
GTTTGAGGAGTTTGGCCGGGCTTTCCACTGTCCCAAGGACTCACCCATGAACCCTGCCCA
CAAGTGTTCCGTGTGGTGAGCCTGGCTGCCCGCCTGCACGCCCCCACTGCCCCCGCACGA
ATCACCTCCTGCTGGCTACCGGGGCAGGCATGCACCCGGTGCCAGCCCCGCTCTGGGCAC
CACCTGCCTTCCAGCCCCTCCAGGACCCGGTCCCCCTGCTGCCCCTCACTTCAGGAGGGG
CCTGGAGCAGGGTGAGGCTGGACTTTGGGGGGCTGTGAGGGAAATATACTGGGGTCCCCA
GATTCTGCTCTAAGGGGGCCAGACCCTCTGCCAGGCTGGATTGTACGGGCCCCACCTTCG
CTGTGTTCTTGCTGCAAAGTCTGGTCAATAAATCACTGCACTGTTAAAAAAAAAAAAAAA
AA
Restriction Sites NotI-NotI |
ACCN NM_004826
Insert Size 2900 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a good match to the protein associated to this reference except for one SNP.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004826.1, NP_004817.1
RefSeq Size 2872 bp
RefSeq ORF 2328 bp
Locus ID 9427
UniProt ID O95672
Cytogenetics 2q37.1
Protein Families Druggable Genome, Protease, Transmembrane
Summary This gene encodes a member of the M13 family of endopeptidases. Members of this family are zinc-containing type II integral-membrane proteins that are important regulators of neuropeptide and peptide hormone activity. Mutations in this gene are associated with autosomal recessive distal arthrogryposis, type 5D. This gene has multiple pseudogenes on chromosome 2. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Mar 2014
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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"NM_004826" in other vectors (4)
SKU Description Size Price
RC208405 ECEL1 (Myc-DDK-tagged)-Human endothelin converting enzyme-like 1 (ECEL1) 10 ug
$1,064.00
RC208405L3 Lenti ORF clone of Human endothelin converting enzyme-like 1 (ECEL1), Myc-DDK-tagged 10 ug
$1,364.00
RC208405L4 Lenti ORF clone of Human endothelin converting enzyme-like 1 (ECEL1), mGFP tagged 10 ug
$1,364.00
RG208405 ECEL1 (tGFP-tagged) - Human endothelin converting enzyme-like 1 (ECEL1) 10 ug
$1,264.00

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