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NOXA1 (NM_006647) Human Tagged ORF Clone

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SKU
RC208357
NOXA1 (Myc-DDK-tagged)-Human NADPH oxidase activator 1 (NOXA1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "NOXA1 or NM_006647"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NOXA1
Synonyms NY-CO-31; p51NOX; SDCCAG31
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208357 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCTCTGGGGGACCTGGTGCGCGCCTGGCACCTGGGCGCGCAGGCTGTGGATCGTGGGGACTGGG
CCCGCGCCTTGCACCTCTTCTCGGGCGTCCCGGCGCCGCCCGCCAGGCTGTGCTTCAACGCGGGCTGCGT
GCACCTGCTGGCCGGGGACCCCGAGGCCGCGCTGCGGGCATTTGACCAAGCCGTGACCAAGGACACCTGC
ATGGCGGTTGGCTTCTTCCAGCGAGGAGTGGCCAACTTCCAGCTGGCAAGGTTCCAGGAGGCTCTGTCTG
ACTTCTGGCTGGCCCTGGAGCAGCTGAGGGGCCACGCTGCCATCGACTACACGCAGCTGGGCCTGCGGTT
CAAGCTGCAAGCCTGGGAGGTGCTACACAATGTGGCGTCGGCACAGTGCCAGCTGGGGCTCTGGACAGAG
GCGGCCAGCAGCCTAAGGGAGGCCATGTCCAAGTGGCCGGAGGGGTCCCTGAATGGCCTGGACTCAGCCC
TGGACCAAGTGCAGAGACGGGGCTCACTGCCGCCACGGCAGGTCCCCAGGGGCGAGGTCTTCCGGCCCCA
CCGGTGGCACCTGAAGCACTTGGAGCCCGTGGATTTCCTGGGCAAGGCCAAGGTGGTGGCCTCTGCCATC
CCCGACGACCAGGGCTGGGGCGTCCGCCCTCAGCAGCCACAGGGACCAGGAGCGAACCATGATGCCAGGT
CCCTAATCATGGACTCCCCAAGAGCTGGCACCCACCAGGGCCCCCTCGATGCAGAGACAGAGGTCGGTGC
TGACCGCTGCACGTCGACTGCCTACCAGGAGCAGAGGCCCCAGGTGGAGCAAGTTGGCAAACAGGCTCCT
CTCTCCCCAGGGCTGCCGGCAATGGGGGGGCCTGGCCCCGGCCCCTGTGAGGACCCCGCGGGTGCTGGGG
GAGCAGGTGCAGGGGGCTCCGAGCCCCTGGTGACTGTCACCGTGCAGTGCGCCTTCACAGTGGCCCTGAG
GGCACGAAGAGGAGCCGACCTGTCCAGCCTGCGGGCACTGCTGGGCCAAGCCCTCCCTCACCAGGCCCAG
CTTGGGCAACTCAGTTACCTAGCCCCAGGTGAGGACGGGCACTGGGTCCCCATCCCCGAGGAGGAGTCGC
TGCAGAGGGCCTGGCAGGACGCAGCTGCCTGCCCCAGGGGGCTGCAGCTGCAGTGCAGGGGAGCCGGGGG
TCGGCCGGTCCTCTACCAGGTGGTGGCCCAGCACAGCTACTCCGCCCAGGGGCCAGAGGACCTGGGCTTC
CGACAGGGGGACACGGTGGACGTCCTGTGTGAAGAGCCCGATGTCCCCCTTGCAGTGGACCAGGCATGGC
TGGAGGGCCACTGTGACGGCCGCATCGGCATCTTCCCCAAGTGCTTCGTGGTCCCCGCCGGCCCTCGGAT
GTCAGGAGCCCCCGGCCGCCTGCCCCGATCCCAGCAGGGAGATCAGCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208357 protein sequence
Red=Cloning site Green=Tags(s)

MASLGDLVRAWHLGAQAVDRGDWARALHLFSGVPAPPARLCFNAGCVHLLAGDPEAALRAFDQAVTKDTC
MAVGFFQRGVANFQLARFQEALSDFWLALEQLRGHAAIDYTQLGLRFKLQAWEVLHNVASAQCQLGLWTE
AASSLREAMSKWPEGSLNGLDSALDQVQRRGSLPPRQVPRGEVFRPHRWHLKHLEPVDFLGKAKVVASAI
PDDQGWGVRPQQPQGPGANHDARSLIMDSPRAGTHQGPLDAETEVGADRCTSTAYQEQRPQVEQVGKQAP
LSPGLPAMGGPGPGPCEDPAGAGGAGAGGSEPLVTVTVQCAFTVALRARRGADLSSLRALLGQALPHQAQ
LGQLSYLAPGEDGHWVPIPEEESLQRAWQDAAACPRGLQLQCRGAGGRPVLYQVVAQHSYSAQGPEDLGF
RQGDTVDVLCEEPDVPLAVDQAWLEGHCDGRIGIFPKCFVVPAGPRMSGAPGRLPRSQQGDQP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006647
ORF Size 1449 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006647.2
RefSeq Size 1650 bp
RefSeq ORF 1452 bp
Locus ID 10811
UniProt ID Q86UR1, Q86UR1-2
Cytogenetics 9q34.3
MW 51.7 kDa
Summary This gene encodes a protein which activates NADPH oxidases, enzymes which catalyze a reaction generating reactive oxygen species. The encoded protein contains four N-terminal tetratricopeptide domains and a C-terminal Src homology 3 domain. Interaction between the encoded protein and proteins in the oxidase regulatory complex occur via the tetratricopeptide domains. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Dec 2011
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Documents
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"NM_006647" in other vectors (6)
SKU Description Size Price
RC208357L1 Lenti ORF clone of Human NADPH oxidase activator 1 (NOXA1), Myc-DDK-tagged 10 ug
$757.00
RC208357L2 Lenti ORF clone of Human NADPH oxidase activator 1 (NOXA1), mGFP tagged 10 ug
$757.00
RC208357L3 Lenti ORF clone of Human NADPH oxidase activator 1 (NOXA1), Myc-DDK-tagged 10 ug
$757.00
RC208357L4 Lenti ORF clone of Human NADPH oxidase activator 1 (NOXA1), mGFP tagged 10 ug
$757.00
RG208357 NOXA1 (tGFP-tagged) - Human NADPH oxidase activator 1 (NOXA1) 10 ug
$489.00 $657.00
SC126108 NOXA1 (untagged)-Human NADPH oxidase activator 1 (NOXA1) 10 ug
$457.00

Plasmid Map for RC208357

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208357
Circular map for RC208357

file-alt Citations

Peptide screen identifies a new NADPH oxidase inhibitor: impact on cell migration and invasion
Mousslim, Pagano, Andreotti et al
Eur. J. Pharmacol. (2016)
AI Summary: OriGene products contributed to this study by providing the pCMV6-DDK tagged plasmids for human Noxo1 and human Noxa1, which were used to reconstitute the Nox1/Noxo1/Noxa1 complex and assess the inhibitory effects of the identified peptide NF02 on NADPH oxidase activity.
Snippet: pCMV6-DDK tagged plasmid for human Noxo1 and human Noxa1 wereobtained from OriGene Technologies Inc. (Rockville, MD, USA). Anti-Nox1 antibodysc-5821 (tebu-bio, Le Perray-en-Yvelines, France), anti-DDK antibody ... Cite SaveSaving
Showing 1-1 of 1 papers.
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