NOXA1 (NM_006647) Human Tagged ORF Clone

  • TrueORF®
SKU
RG208357
NOXA1 (tGFP-tagged) - Human NADPH oxidase activator 1 (NOXA1)
$489.00 $657.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NOXA1
Synonyms NY-CO-31; p51NOX; SDCCAG31
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG208357 representing NM_006647
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCTCTGGGGGACCTGGTGCGCGCCTGGCACCTGGGCGCGCAGGCTGTGGATCGTGGGGACTGGG
CCCGCGCCTTGCACCTCTTCTCGGGCGTCCCGGCGCCGCCCGCCAGGCTGTGCTTCAACGCGGGCTGCGT
GCACCTGCTGGCCGGGGACCCCGAGGCCGCGCTGCGGGCATTTGACCAAGCCGTGACCAAGGACACCTGC
ATGGCGGTTGGCTTCTTCCAGCGAGGAGTGGCCAACTTCCAGCTGGCAAGGTTCCAGGAGGCTCTGTCTG
ACTTCTGGCTGGCCCTGGAGCAGCTGAGGGGCCACGCTGCCATCGACTACACGCAGCTGGGCCTGCGGTT
CAAGCTGCAAGCCTGGGAGGTGCTACACAATGTGGCGTCGGCACAGTGCCAGCTGGGGCTCTGGACAGAG
GCGGCCAGCAGCCTAAGGGAGGCCATGTCCAAGTGGCCGGAGGGGTCCCTGAATGGCCTGGACTCAGCCC
TGGACCAAGTGCAGAGACGGGGCTCACTGCCGCCACGGCAGGTCCCCAGGGGCGAGGTCTTCCGGCCCCA
CCGGTGGCACCTGAAGCACTTGGAGCCCGTGGATTTCCTGGGCAAGGCCAAGGTGGTGGCCTCTGCCATC
CCCGACGACCAGGGCTGGGGCGTCCGCCCTCAGCAGCCACAGGGACCAGGAGCGAACCATGATGCCAGGT
CCCTAATCATGGACTCCCCAAGAGCTGGCACCCACCAGGGCCCCCTCGATGCAGAGACAGAGGTCGGTGC
TGACCGCTGCACGTCGACTGCCTACCAGGAGCAGAGGCCCCAGGTGGAGCAAGTTGGCAAACAGGCTCCT
CTCTCCCCAGGGCTGCCGGCAATGGGGGGGCCTGGCCCCGGCCCCTGTGAGGACCCCGCGGGTGCTGGGG
GAGCAGGTGCAGGGGGCTCCGAGCCCCTGGTGACTGTCACCGTGCAGTGCGCCTTCACAGTGGCCCTGAG
GGCACGAAGAGGAGCCGACCTGTCCAGCCTGCGGGCACTGCTGGGCCAAGCCCTCCCTCACCAGGCCCAG
CTTGGGCAACTCAGTTACCTAGCCCCAGGTGAGGACGGGCACTGGGTCCCCATCCCCGAGGAGGAGTCGC
TGCAGAGGGCCTGGCAGGACGCAGCTGCCTGCCCCAGGGGGCTGCAGCTGCAGTGCAGGGGAGCCGGGGG
TCGGCCGGTCCTCTACCAGGTGGTGGCCCAGCACAGCTACTCCGCCCAGGGGCCAGAGGACCTGGGCTTC
CGACAGGGGGACACGGTGGACGTCCTGTGTGAAGAGCCCGATGTCCCCCTTGCAGTGGACCAGGCATGGC
TGGAGGGCCACTGTGACGGCCGCATCGGCATCTTCCCCAAGTGCTTCGTGGTCCCCGCCGGCCCTCGGAT
GTCAGGAGCCCCCGGCCGCCTGCCCCGATCCCAGCAGGGAGATCAGCCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG208357 representing NM_006647
Red=Cloning site Green=Tags(s)

MASLGDLVRAWHLGAQAVDRGDWARALHLFSGVPAPPARLCFNAGCVHLLAGDPEAALRAFDQAVTKDTC
MAVGFFQRGVANFQLARFQEALSDFWLALEQLRGHAAIDYTQLGLRFKLQAWEVLHNVASAQCQLGLWTE
AASSLREAMSKWPEGSLNGLDSALDQVQRRGSLPPRQVPRGEVFRPHRWHLKHLEPVDFLGKAKVVASAI
PDDQGWGVRPQQPQGPGANHDARSLIMDSPRAGTHQGPLDAETEVGADRCTSTAYQEQRPQVEQVGKQAP
LSPGLPAMGGPGPGPCEDPAGAGGAGAGGSEPLVTVTVQCAFTVALRARRGADLSSLRALLGQALPHQAQ
LGQLSYLAPGEDGHWVPIPEEESLQRAWQDAAACPRGLQLQCRGAGGRPVLYQVVAQHSYSAQGPEDLGF
RQGDTVDVLCEEPDVPLAVDQAWLEGHCDGRIGIFPKCFVVPAGPRMSGAPGRLPRSQQGDQP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006647
ORF Size 1449 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006647.2
RefSeq Size 1650 bp
RefSeq ORF 1452 bp
Locus ID 10811
UniProt ID Q86UR1
Cytogenetics 9q34.3
Summary This gene encodes a protein which activates NADPH oxidases, enzymes which catalyze a reaction generating reactive oxygen species. The encoded protein contains four N-terminal tetratricopeptide domains and a C-terminal Src homology 3 domain. Interaction between the encoded protein and proteins in the oxidase regulatory complex occur via the tetratricopeptide domains. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Dec 2011
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Documents
Resources
"NM_006647" in other vectors (6)
SKU Description Size Price
RC208357 NOXA1 (Myc-DDK-tagged)-Human NADPH oxidase activator 1 (NOXA1) 10 ug
$457.00
RC208357L1 Lenti ORF clone of Human NADPH oxidase activator 1 (NOXA1), Myc-DDK-tagged 10 ug
$757.00
RC208357L2 Lenti ORF clone of Human NADPH oxidase activator 1 (NOXA1), mGFP tagged 10 ug
$757.00
RC208357L3 Lenti ORF clone of Human NADPH oxidase activator 1 (NOXA1), Myc-DDK-tagged 10 ug
$757.00
RC208357L4 Lenti ORF clone of Human NADPH oxidase activator 1 (NOXA1), mGFP tagged 10 ug
$757.00
SC126108 NOXA1 (untagged)-Human NADPH oxidase activator 1 (NOXA1) 10 ug
$457.00

Plasmid Map for RG208357

Download sequence for

Cloning scheme for RG208357

Circular map for RG208357

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