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PLCD1 (NM_006225) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC208263
PLCD1 (Myc-DDK-tagged)-Human phospholipase C, delta 1 (PLCD1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $692.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "PLCD1 or NM_006225"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PLCD1
Synonyms NDNC3; PLC-III
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006225
ORF Size 2268 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006225.4
RefSeq Size 2683 bp
RefSeq ORF 2271 bp
Locus ID 5333
UniProt ID P51178
Cytogenetics 3p22.2
Protein Families Druggable Genome
Protein Pathways Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
MW 85.7 kDa
Summary This gene encodes a member of the phospholipase C family. Phospholipase C isozymes play critical roles in intracellular signal transduction by catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers diacylglycerol (DAG) and inositol triphosphate (IP3). The encoded protein functions as a tumor suppressor in several types of cancer, and mutations in this gene are a cause of hereditary leukonychia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Dec 2011
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"NM_006225" in other vectors (4)
SKU Description Size Price
RC208263L3 Lenti ORF clone of Human phospholipase C, delta 1 (PLCD1), transcript variant 2, Myc-DDK-tagged 10 ug
$992.00
RC208263L4 Lenti ORF clone of Human phospholipase C, delta 1 (PLCD1), transcript variant 2, mGFP tagged 10 ug
$992.00
RG208263 PLCD1 (tGFP-tagged) - Human phospholipase C, delta 1 (PLCD1), transcript variant 2 10 ug
$789.00 $892.00
SC303752 PLCD1 (untagged)-Human phospholipase C, delta 1 (PLCD1), transcript variant 2 10 ug
$693.00

Plasmid Map for RC208263

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Cloning scheme for RC208263
Circular map for RC208263

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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