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PLCD1 (NM_006225) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG208263
PLCD1 (tGFP-tagged) - Human phospholipase C, delta 1 (PLCD1), transcript variant 2
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$789.00 $892.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PLCD1
Synonyms NDNC3; PLC-III
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG208263 representing NM_006225
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTCGGGCCGGGACTTCCTGACCCTGCACGGCCTACAGGATGATGAGGATCTACAGGCGCTGCTGA
AGGGCAGCCAGCTCCTGAAGGTGAAGTCCAGCTCATGGAGGAGAGAGCGCTTCTACAAGTTGCAGGAGGA
CTGCAAGACCATCTGGCAGGAGTCCCGCAAGGTCATGCGGACCCCGGAGTCCCAGCTGTTCTCCATCGAG
GACATTCAGGAGGTGCGAATGGGGCACCGCACGGAGGGTCTGGAGAAGTTCGCCCGTGATGTGCCCGAGG
ACCGCTGCTTCTCCATTGTCTTCAAGGACCAGCGCAATACACTAGACCTCATCGCCCCATCGCCAGCTGA
TGCCCAGCACTGGGTGCTGGGGCTGCACAAGATCATCCACCACTCAGGCTCCATGGACCAGCGTCAGAAG
CTACAGCACTGGATTCACTCCTGCTTGCGAAAAGCTGACAAAAACAAGGACAACAAGATGAGCTTCAAGG
AGCTGCAGAACTTCCTGAAGGAGCTCAACATCCAGGTGGACGACAGCTATGCCCGGAAGATCTTCAGGGA
GTGTGACCACTCCCAGACAGACTCCCTGGAGGACGAGGAGATTGAGGCCTTCTACAAGATGCTGACCCAG
CGGGTGGAGATCGACCGCACCTTCGCCGAGGCCGCGGGCTCAGGGGAGACTCTGTCGGTGGATCAGTTAG
TGACGTTCCTGCAGCACCAGCAGCGGGAGGAGGCGGCAGGGCCTGCGCTGGCCCTCTCCCTCATTGAGCG
CTACGAGCCCAGCGAGACTGCCAAGGCGCAGCGGCAGATGACCAAGGACGGCTTCCTCATGTACTTACTG
TCGGCTGACGGCAGCGCCTTCAGCCTGGCACACCGCCGTGTCTACCAGGACATGGGCCAGCCACTTAGCC
ACTACCTGGTGTCCTCTTCACACAACACCTACCTGCTGGAGGACCAGCTAGCCGGGCCCAGCAGCACTGA
AGCCTACATCCGGGCACTGTGCAAAGGCTGCCGATGCCTGGAGCTTGACTGCTGGGACGGGCCCAACCAG
GAACCAATCATCTACCACGGCTATACTTTCACTTCCAAGATCCTCTTCTGCGATGTGCTCAGGGCCATCC
GGGACTATGCCTTCAAGGCGTCCCCCTACCCTGTCATCCTATCCCTGGAGAACCACTGCACACTGGAGCA
GCAGCGCGTGATGGCGCGGCACCTGCATGCCATCCTGGGCCCCATGCTGTTGAACCGACCACTGGATGGG
GTCACCAACAGCCTGCCCTCCCCTGAGCAACTGAAGGGGAAGATCCTGCTGAAGGGGAAGAAGCTCGGGG
GGCTCCTGCCCCCTGGAGGGGAGGGTGGCCCTGAGGCCACTGTGGTGTCAGACGAAGACGAGGCTGCTGA
GATGGAGGATGAGGCAGTGAGGAGCCGTGTGCAGCACAAGCCCAAGGAGGACAAGCTCAGGCTAGCACAG
GAGCTCTCTGACATGGTCATTTACTGCAAGAGTGTCCACTTTGGGGGCTTCTCCAGTCCTGGCACCCCTG
GACAGGCCTTCTACGAGATGGCGTCCTTCTCTGAGAACCGTGCCCTTCGACTGCTCCAAGAATCAGGAAA
CGGCTTTGTCCGCCACAACGTGGGGCACCTGAGCAGAATCTACCCGGCTGGATGGAGAACAGACTCCTCC
AACTACAGCCCCGTGGAGATGTGGAATGGGGGCTGCCAGATCGTGGCCCTGAATTTCCAGACACCTGGGC
CAGAGATGGACGTGTACCAGGGCCGCTTCCAGGACAACGGGGCCTGTGGGTACGTGCTGAAGCCCGCCTT
CCTGCGAGACCCCAACGGCACCTTTAACCCCCGCGCCCTGGCTCAGGGGCCCTGGTGGGCACGGAAGCGG
CTCAACATCAGGGTCATTTCGGGGCAGCAGCTGCCAAAAGTCAACAAGAATAAGAATTCAATTGTGGACC
CCAAAGTGACAGTGGAGATCCATGGCGTGAGCCGGGACGTGGCCAGCCGCCAGACTGCTGTCATCACCAA
CAATGGTTTCAACCCATGGTGGGACACGGAGTTTGCGTTTGAGGTAGTTGTGCCTGACCTTGCCCTCATC
CGCTTCTTGGTGGAAGATTATGATGCCTCCTCCAAGAATGACTTCATTGGCCAGAGTACCATCCCCTTGA
ACAGCCTCAAGCAAGGATACCGCCATGTCCACCTCATGTCTAAGAACGGGGACCAGCATCCATCAGCCAC
CCTCTTTGTGAAGATCTCCCTCCAGGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG208263 representing NM_006225
Red=Cloning site Green=Tags(s)

MDSGRDFLTLHGLQDDEDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRTPESQLFSIE
DIQEVRMGHRTEGLEKFARDVPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVLGLHKIIHHSGSMDQRQK
LQHWIHSCLRKADKNKDNKMSFKELQNFLKELNIQVDDSYARKIFRECDHSQTDSLEDEEIEAFYKMLTQ
RVEIDRTFAEAAGSGETLSVDQLVTFLQHQQREEAAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLL
SADGSAFSLAHRRVYQDMGQPLSHYLVSSSHNTYLLEDQLAGPSSTEAYIRALCKGCRCLELDCWDGPNQ
EPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCTLEQQRVMARHLHAILGPMLLNRPLDG
VTNSLPSPEQLKGKILLKGKKLGGLLPPGGEGGPEATVVSDEDEAAEMEDEAVRSRVQHKPKEDKLRLAQ
ELSDMVIYCKSVHFGGFSSPGTPGQAFYEMASFSENRALRLLQESGNGFVRHNVGHLSRIYPAGWRTDSS
NYSPVEMWNGGCQIVALNFQTPGPEMDVYQGRFQDNGACGYVLKPAFLRDPNGTFNPRALAQGPWWARKR
LNIRVISGQQLPKVNKNKNSIVDPKVTVEIHGVSRDVASRQTAVITNNGFNPWWDTEFAFEVVVPDLALI
RFLVEDYDASSKNDFIGQSTIPLNSLKQGYRHVHLMSKNGDQHPSATLFVKISLQD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006225
ORF Size 2268 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006225.4
RefSeq Size 2707 bp
RefSeq ORF 2271 bp
Locus ID 5333
UniProt ID P51178
Cytogenetics 3p22.2
Protein Families Druggable Genome
Protein Pathways Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
Summary This gene encodes a member of the phospholipase C family. Phospholipase C isozymes play critical roles in intracellular signal transduction by catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers diacylglycerol (DAG) and inositol triphosphate (IP3). The encoded protein functions as a tumor suppressor in several types of cancer, and mutations in this gene are a cause of hereditary leukonychia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Dec 2011
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"NM_006225" in other vectors (4)
SKU Description Size Price
RC208263 PLCD1 (Myc-DDK-tagged)-Human phospholipase C, delta 1 (PLCD1), transcript variant 2 10 ug
$589.00 $692.00
RC208263L3 Lenti ORF clone of Human phospholipase C, delta 1 (PLCD1), transcript variant 2, Myc-DDK-tagged 10 ug
$992.00
RC208263L4 Lenti ORF clone of Human phospholipase C, delta 1 (PLCD1), transcript variant 2, mGFP tagged 10 ug
$992.00
SC303752 PLCD1 (untagged)-Human phospholipase C, delta 1 (PLCD1), transcript variant 2 10 ug
$693.00

Plasmid Map for RG208263

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG208263
Circular map for RG208263

file-alt Citations

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