PRKAR1B (NM_002735) Human Tagged ORF Clone

SKU RC207809

PRKAR1B (Myc-DDK-tagged)-Human protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PRKAR1B
• Synonyms: PRKAR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207809 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCTCCCCGCCCGCCTGCCCCTCGGAGGAGGACGAGAGCCTGAAGGGCTGTGAGCTGTACGTGCAGC; TGCACGGGATCCAGCAGGTCCTCAAAGACTGTATCGTCCACCTCTGCATCTCCAAGCCCGAACGCCCCAT; GAAGTTCCTCCGGGAGCACTTCGAGAAGCTGGAGAAGGAAGAAAACAGGCAGATTTTGGCGCGGCAAAAG; TCAAACTCACAGTCGGACTCCCATGATGAGGAGGTGTCGCCCACCCCCCCGAACCCTGTGGTGAAGGCCC; GCCGCCGGCGAGGAGGCGTGAGTGCCGAGGTGTACACCGAGGAGGACGCCGTGTCCTACGTCAGGAAGGT; GATTCCCAAGGACTACAAAACCATGACTGCGCTGGCCAAGGCCATCTCCAAGAACGTGCTCTTCGCTCAC; CTGGATGACAACGAGAGGAGTGACATATTCGATGCCATGTTCCCTGTCACTCACATCGCTGGGGAGACTG; TTATACAGCAAGGGAATGAAGGAGACAACTTCTATGTCGTTGATCAAGGGGAAGTGGATGTGTACGTGAA; CGGAGAGTGGGTGACCAACATCAGCGAGGGAGGCAGCTTCGGGGAGCTGGCGCTCATCTACGGCACCCCC; AGGGCTGCGACCGTGAAAGCCAAGACGGACCTCAAGCTCTGGGGGATCGACCGGGACAGCTACCGGCGCA; TCCTTATGGGCAGCACGCTGAGGAAACGCAAGATGTACGAGGAGTTCCTCAGCAAGGTCTCCATCCTAGA; GTCCCTGGAGAAGTGGGAGCGTCTGACCGTGGCGGATGCGCTGGAGCCCGTCCAGTTTGAAGATGGAGAG; AAAATTGTGGTCCAGGGAGAGCCTGGGGACGACTTTTACATCATCACGGAGGGCACCGCGTCCGTGCTGC; AGCGCCGGTCCCCCAATGAGGAGTACGTGGAGGTGGGGCGCCTGGGACCCTCTGACTACTTCGGGGAGAT; TGCACTGCTGCTGAACCGGCCCCGGGCGGCCACTGTCGTGGCCCGGGGGCCCCTCAAGTGTGTGAAGCTG; GACCGGCCCCGCTTCGAGCGTGTGCTGGGGCCCTGCTCTGAGATCCTCAAGAGGAACATTCAGCGTTACA; ACAGCTTCATCTCCCTCACCGTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207809 protein sequence; Red=Cloning site Green=Tags(s); ; MASPPACPSEEDESLKGCELYVQLHGIQQVLKDCIVHLCISKPERPMKFLREHFEKLEKEENRQILARQK; SNSQSDSHDEEVSPTPPNPVVKARRRRGGVSAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKNVLFAH; LDDNERSDIFDAMFPVTHIAGETVIQQGNEGDNFYVVDQGEVDVYVNGEWVTNISEGGSFGELALIYGTP; RAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGE; KIVVQGEPGDDFYIITEGTASVLQRRSPNEEYVEVGRLGPSDYFGEIALLLNRPRAATVVARGPLKCVKL; DRPRFERVLGPCSEILKRNIQRYNSFISLTV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002735
• ORF Size: 1143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002735.2
• RefSeq Size: 2512 bp
• RefSeq ORF: 1146 bp
• Locus ID: 5575
• UniProt ID: P31321
• Cytogenetics: 7p22.3
• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis, Insulin signaling pathway
• MW: 43.1 kDa
• Summary: The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2015]