TAB3 (NM_152787) Human Tagged ORF Clone

SKU RC207646

TAB3 (Myc-DDK-tagged)-Human TGF-beta activated kinase 1/MAP3K7 binding protein 3 (TAB3)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TAB3
• Synonyms: MAP3K7IP3; NAP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207646 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGCAAAGCAGCCCACAGCTTGATATTCAGGTTCTCCATGATCTTCGACAACGTTTCCCTGAAATTC; CAGAGGGCGTGGTGTCTCAGTGCATGTTACAGAATAACAACAATCTTGAAGCCTGTTGCCGAGCCCTTTC; CCAGGAGAGTAGCAAATACTTATATATGGAATACCATAGTCCAGATGACAATAGGATGAATAGAAATCGC; CTTTTACATATTAACCTGGGTATCCATTCTCCTAGTAGCTATCACCCAGGAGATGGAGCCCAACTTAATG; GTGGTCGAACACTGGTACATAGCTCAAGTGATGGACATATTGATCCTCAGCATGCAGCAGGTAAACAGCT; GATATGTTTAGTTCAAGAACCACACTCAGCTCCAGCTGTTGTTGCTGCTACTCCCAACTACAATCCATTT; TTTATGAACGAACAGAACAGAAGTGCAGCTACTCCTCCTTCACAACCACCTCAACAGCCATCTTCCATGC; AAACAGGAATGAATCCGTCTGCTATGCAAGGGCCTTCACCACCACCGCCACCTCCTTCATACATGCACAT; ACCTCGGTATAGTACAAATCCAATTACTGTTACAGTATCCCAGAACCTCCCTTCTGGACAGACTGTACCA; AGAGCTTTACAAATTCTTCCACAAATTCCAAGCAATCTCTATGGGTCTCCTGGTTCTATTTATATTAGAC; AGACATCTCAGAGTTCATCAGGAAGACAAACTCCTCAGAGTACGCCGTGGCAGTCCTCACCACAGGGCCC; AGTGCCTCACTATAGCCAGCGTCCTTTACCTGTTTATCCACACCAACAGAACTATCAGCCTTCTCAGTAT; TCTCCCAAACAGCAGCAGATCCCTCAGTCTGCTTACCATTCACCACCTCCTTCTCAATGTCCTTCACCCT; TCAGCTCTCCACAGCATCAAGTGCAACCTTCCCAGTTGGGCCACATCTTTATGCCACCTAGTCCTTCAAC; TACTCCACCCCATCCATATCAACAAGGACCTCCTAGCTATCAGAAACAGGGAAGCCATTCAGTAGCCTAT; CTTCCATACACAGCATCTAGTTTATCCAAAGGTTCCATGAAGAAGATAGAAATTACAGTTGAACCTTCTC; AAAGACCTGGGACAGCAATTAATAGGAGTCCTTCACCCATCAGTAATCAACCATCTCCACGGAATCAACA; CTCACTGTACACAGCCACCACGCCACCTTCAAGTTCTCCTTCAAGAGGGATATCTAGTCAACCAAAACCT; CCATTTAGTGTTAATCCTGTGTATATTACATATACACAGCCAACTGGACCTTCTTGTACTCCATCACCAT; CTCCTCGAGTGATACCAAACCCAACTACAGTTTTTAAAATTACCGTAGGCCGAGCAACGACTGAAAATCT; TTTAAATTTAGTGGACCAAGAAGAGCGCTCTGCAGCACCAGAACCTATTCAGCCCATTTCAGTGATACCA; GGCTCTGGGGGAGAAAAGGGAAGCCATAAATATCAGAGAAGTTCTAGTTCTGGATCAGATGACTATGCCT; ACACACAAGCCTTGCTGTTACATCAACGAGCAAGGATGGAGAGGTTAGCAAAGCAACTGAAACTTGAGAA; AGAGGAGCTAGAGCGGTTGAAGTCTGAAGTTAATGGTATGGAGCATGACCTGATGCAGAGACGGCTCAGA; AGAGTCAGCTGCACCACTGCGATCCCTACGCCTGAGGAAATGACAAGATTGAGAAGCATGAACAGACAAC; TCCAGATAAATGTTGACTGTACACTGAAAGAAGTTGACCTCCTTCAATCTAGAGGAAACTTTGATCCAAA; AGCCATGAATAATTTTTATGACAACATAGAACCTGGCCCAGTTGTACCACCCAAGCCATCTAAAAAAGAC; TCCTCAGACCCCTGCACAATTGAGAGAAAAGCCCGAAGAATTAGCGTGACCTCCAAAGTACAGGCAGACA; TCCATGACACCCAGGCAGCAGCTGCAGATGAGCATCGAACTGGCTCCACACAAAGTCCTCGGACACAACC; TCGAGATGAAGACTACGAAGGGGCTCCATGGAATTGTGATAGCTGCACCTTTCTTAACCACCCAGCACTA; AATCGCTGTGAGCAGTGCGAGATGCCACGGTACACC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207646 protein sequence; Red=Cloning site Green=Tags(s); ; MAQSSPQLDIQVLHDLRQRFPEIPEGVVSQCMLQNNNNLEACCRALSQESSKYLYMEYHSPDDNRMNRNR; LLHINLGIHSPSSYHPGDGAQLNGGRTLVHSSSDGHIDPQHAAGKQLICLVQEPHSAPAVVAATPNYNPF; FMNEQNRSAATPPSQPPQQPSSMQTGMNPSAMQGPSPPPPPPSYMHIPRYSTNPITVTVSQNLPSGQTVP; RALQILPQIPSNLYGSPGSIYIRQTSQSSSGRQTPQSTPWQSSPQGPVPHYSQRPLPVYPHQQNYQPSQY; SPKQQQIPQSAYHSPPPSQCPSPFSSPQHQVQPSQLGHIFMPPSPSTTPPHPYQQGPPSYQKQGSHSVAY; LPYTASSLSKGSMKKIEITVEPSQRPGTAINRSPSPISNQPSPRNQHSLYTATTPPSSSPSRGISSQPKP; PFSVNPVYITYTQPTGPSCTPSPSPRVIPNPTTVFKITVGRATTENLLNLVDQEERSAAPEPIQPISVIP; GSGGEKGSHKYQRSSSSGSDDYAYTQALLLHQRARMERLAKQLKLEKEELERLKSEVNGMEHDLMQRRLR; RVSCTTAIPTPEEMTRLRSMNRQLQINVDCTLKEVDLLQSRGNFDPKAMNNFYDNIEPGPVVPPKPSKKD; SSDPCTIERKARRISVTSKVQADIHDTQAAAADEHRTGSTQSPRTQPRDEDYEGAPWNCDSCTFLNHPAL; NRCEQCEMPRYT; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152787
• ORF Size: 2136 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152787.5
• RefSeq Size: 6798 bp
• RefSeq ORF: 2139 bp
• Locus ID: 257397
• UniProt ID: Q8N5C8
• Cytogenetics: Xp21.2
• Domains: CUE, zf-RanBP
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• MW: 78.7 kDa
• Summary: The product of this gene functions in the NF-kappaB signal transduction pathway. The encoded protein, and the similar and functionally redundant protein MAP3K7IP2/TAB2, forms a ternary complex with the protein kinase MAP3K7/TAK1 and either TRAF2 or TRAF6 in response to stimulation with the pro-inflammatory cytokines TNF or IL-1. Subsequent MAP3K7/TAK1 kinase activity triggers a signaling cascade leading to activation of the NF-kappaB transcription factor. The human genome contains a related pseudogene. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]