Peripherin (PRPH) (NM_006262) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Peripherin |
| Synonyms | NEF4; PRPH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_006262 |
| ORF Size | 1410 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006262.4 |
| RefSeq Size | 1833 bp |
| RefSeq ORF | 1413 bp |
| Locus ID | 5630 |
| UniProt ID | P41219 |
| Cytogenetics | 12q13.12 |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS) |
| MW | 53.7 kDa |
| Summary | This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006262" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207561L1 | Lenti ORF clone of Human peripherin (PRPH), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC207561L2 | Lenti ORF clone of Human peripherin (PRPH), mGFP tagged | 10 ug |
$757.00
|
|
| RC207561L3 | Lenti ORF clone of Human peripherin (PRPH), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC207561L4 | Lenti ORF clone of Human peripherin (PRPH), mGFP tagged | 10 ug |
$757.00
|
|
| RG207561 | PRPH (tGFP-tagged) - Human peripherin (PRPH) | 10 ug |
$489.00
$657.00
|
|
| SC123831 | PRPH (untagged)-Human peripherin (PRPH) | 10 ug |
$457.00
|
|
Citations
The Type III Intermediate Filament Protein Peripherin Regulates Lysosomal Degradation Activity and Autophagy
Romano, Cordella, Bucci
Int J Mol Sci (2025) 26 (2) DOI: 10.3390/ijms26020549
Romano, Cordella, Bucci
Int J Mol Sci (2025) 26 (2) DOI: 10.3390/ijms26020549
Cited by 5
AI Summary: OriGene's myc-tagged peripherin plasmid (RC207561) was used in rescue experiments to restore peripherin expression after silencing, enabling the authors to assess the specific role of peripherin in regulating lysosomal activity and autophagy.
Snippet: Peripherin belongs to heterogeneous class III of intermediate filaments, and it is the only intermediate filament protein selectively expressed in the neurons of the;GAATT-3′ and antisense sequence 5′-UUCUUCUUCUAACCUCUGCTT-3′). In rescue experiments, 48 h after silencing, cells were transfected with a plasmid coding for myc-tagged peripherin purchased from Origene (RC207561, Rockville, MD, USA) and using Metafectene Pro from Biontex following the manufacturer’s instructions. …
High serum prevalence of autoreactive IgG antibodies against peripheral nerve structures in patients with neurological post-COVID-19 vaccination syndrome
Arlt, Breuer, Trampenau et al
Front Immunol (2024) 15, 1404800 DOI: 10.3389/fimmu.2024.1404800
Arlt, Breuer, Trampenau et al
Front Immunol (2024) 15, 1404800 DOI: 10.3389/fimmu.2024.1404800
Cited by 10
AI Summary: OriGene products, specifically plasmids encoding Neurofilament heavy chain (NFH; #RC213487), Neurofilament medium chain (NFM; #RC224475), Neurofilament light chain (NFL; #RC205920), and Peripherin (PRPH; #RC207561), were used to identify and confirm autoantibody targets in patients with neurological post-COVID-19 vaccination syndrome.
Snippet: vity against neuronal intermediate filament antigens was adapted from previously published protocols (13). In brief, commercially available plasmids encoding Neurofilament heavy chain (NFH;#RC213487 Origene, Rockville, MD, USA), Neurofilament medium chain (NFM;#RC224475 Origene), Neurofilament light chain (NFL;#RC205920 Origene), Peripherin (PRPH;#RC207561 Origene), and Alpha-Internexin (INA
A PRPH splice-donor variant associates with reduced sural nerve amplitude and risk of peripheral neuropathy
Bjornsdottir, Ivarsdottir, Bjarnadottir et al
Nat Commun (2019) 10 (1), 1777 DOI: 10.1038/s41467-019-09719-4
Bjornsdottir, Ivarsdottir, Bjarnadottir et al
Nat Commun (2019) 10 (1), 1777 DOI: 10.1038/s41467-019-09719-4
AI Summary: OriGene’s PRPH cDNA (NM_006262.3) in the pCMV6-Entry mammalian expression vector (RC207561) was used to generate plasmids for functional studies of PRPH splice variants, enabling the authors to assess the impact of the disease-associated mutation on protein structure and function.
Snippet: ormalized by sequencing depth. Generation of PRPH splice variant plasmids The full-length PRPH cDNA (NM_006262.3) in pCMV6-Entry mammalian expression vector including a Myc-DDK tag was obtained from Origene (RC207561). Plasmids for the 4 bp- and intron-retention splice variants were generated by mutating the full-length PRPH plasmid in two separated PCR reactions using Q5 site-directed mutagene
Charcot-Marie-Tooth type 2B disease-causing RAB7A mutant proteins show altered interaction with the neuronal intermediate filament peripherin
Cogli, Progida, Thomas et al
Acta Neuropathol. (2013) 125 (2), 257-72 DOI: 10.1007/s00401-012-1063-8
Cogli, Progida, Thomas et al
Acta Neuropathol. (2013) 125 (2), 257-72 DOI: 10.1007/s00401-012-1063-8
Cited by 54
AI Summary: OriGene’s pCMV6-AN-HA vector (PS100013) was used to express HA-tagged peripherin, enabling the researchers to study interactions between peripherin and RAB7A proteins in their investigation of Charcot-Marie-Tooth type 2B disease mechanisms.
Snippet: Peri- pherin ORF was transferred using AsiSI and MluI restriction enzymes to pCMV6-AN-HA (Origene, PS100013) to obtain a vector for the expression of HA- tagged peripherin. ... Strikingly, anti-RAB7A antibodies recognized a band Fig.
Showing 1-4 of 4 papers.
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