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Peripherin (PRPH) (NM_006262) Human Untagged Clone

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SKU
SC123831
PRPH (untagged)-Human peripherin (PRPH)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Peripherin
Synonyms NEF4; PRPH1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_006262 edited
GCTCTGCGAACGGTGACTGCCCATCCTTGGCCGCAATGAGCCACCACCCGTCGGGCCTCC
GGGCCGGCTTCAGCTCCACCTCATACCGCCGTACCTTCGGTCCACCGCCCTCACTATCCC
CCGGGGCCTTCTCCTACTCGTCCAGCTCCCGCTTCTCCAGCAGCCGCCTGCTGGGCTCCG
CGTCCCCGAGCTCCTCGGTGCGCCTGGGCAGCTTCCGTAGCCCCCGAGCGGGAGCGGGCG
CCCTCCTGCGCCTGCCCTCGGAGCGCCTCGACTTCTCCATGGCCGAGGCCCTCAACCAGG
AGTTCCTGGCCACGCGCAGCAACGAGAAGCAGGAGCTGCAGGAGCTCAACGACCGCTTCG
CCAACTTCATCGAGAAGGTACGCTTTCTGGAGCAGCAGAACGCGGCCCTGCGCGGGGAGC
TGAGCCAAGCCCGGGGCCAGGAGCCGGCGCGCGCCGACCAGCTGTGCCAGCAGGAGCTGC
GCGAGCTGCGGCGAGAGCTGGAGCTGTTGGGCCGCGAGCGTGACCGGGTGCAGGTGGAGC
GCGACGGGCTGGCGGAGGACCTGGCGGCGCTCAAGCAGAGGTTGGAGGAGGAGACGCGCA
AGCGGGAGGACGCGGAGCACAACCTCGTGCTCTTCCGCAAGGACGTGGACGATGCCACTC
TGTCCCGCCTGGAACTAGAGCGCAAGATTGAGTCTCTGATGGATGAGATTGAGTTCCTCA
AGAAGCTGCACGAGGAGGAGCTGCGAGACCTGCAGGTGAGTGTGGAGAGCCAGCAGGTGC
AGCAGGTGGAGGTGGAAGCCACGGTGAAGCCCGAGCTGACGGCAGCGCTGAGGGACATCC
GCGCGCAGTACGAGAGCATCGCCGCGAAGAACCTGCAGGAGGCGGAGGAGTGGTACAAGT
CCAAGTACGCGGACCTGTCCGACGCTGCCAACCGGAACCACGAGGCCCTGCGCCAGGCCA
AGCAGGAGATGAACGAGTCCCGACGCCAGATCCAGAGTCTAACGTGCGAGGTGGACGGGC
TGCGCGGCACGAACGAGGCGCTGCTCAGGCAGTTGAGAGAGCTGGAGGAGCAGTTCGCCC
TGGAGGCGGGGGGCTACCAGGCGGGCGCTGCGCGGCTCGAGGAGGAGCTGCGACAGCTAA
AAGAGGAGATGGCGCGGCACCTGAGGGAGTACCAGGAGCTCCTCAACGTCAAGATGGCCC
TGGACATCGAGATCGCCACCTACCGCAAGCTGCTGGAGGGCGAGGAGAGCCGGATCTCCG
TGCCCGTCCATTCTTTTGCCTCCTTAAATATAAAGACGACTGTGCCTGAGGTGGAGCCTC
CCCAGGACAGCCACAGCCGGAAGACGGTTCTGATCAAGACCATTGAGACCCGGAATGGGG
AGGTGGTGACAGAGTCCCAGAAGGAGCAGCGCAGTGAGCTGGACAAGTCTTCTGCCCACA
GTTACTGAACCCCTTGGTCCGGAGCCTTGACTCTGCCCTAGGCCTGCTCAAAGCCCAAAC
CCTAAGACCACTCCTGAATTGTCTCCTCTCCCTCTGCATGTGTCTAAAAGGTGGTACCAG
GCATCCCTTTCCTGGCTTATGGCCAAGCCCTACCCGGCCAGCAGTCGCTGGGCCTCTCCC
TGCCCTGACACTTGATGTGACCTATGTGCTTCCCTTTTCATGTCCCGATAAGAAGCCAAT
GATCCCCCCTCAGGACAAATCTACTCCAGCCACGATGAGAAGTGGGTGAGCCAGGGTCTG
AGTTTCACATTTGAACCAAATAAAATGCTGTCAAGAGAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006262 unedited
GTTTGCATTTGTNATACGACTCACTATAGGGCGGCCGCGACTTCCCGGGGTGCTCTGCGA
ACGGTGACTGCCCATCCTTGGCCGCAATGAGCCACCACCCGTCGGGCCTCCGGGCCGGCT
TCAGCTCCACCTCATACCGCCGTACCTTCGGTCCACCGCCCTCACTATCCCCCGGGCCTT
CTCCTACTCGTCCAGCTCCCGCTTCTCCAGCAGCCGCCTGCTGGGCTCCGCGTCCCCGAG
CTCCGTCGTGCGCCTGGGCAGCTTCCGTAGCCCCCGAGCGGGAGCGGGCGCCCTCCTGCG
CCTGCCCTCGGAGCGCCTCGACTTCTCCATGGCCGATGCCCTCAACCAGGAGTTCCTGGC
CACGCGCATCAACGAGAAGCAGGAGCTGCAGGAGCTCAACGACCGCTTCGCCAACTTCAT
CGAGAAGGTACGCTTTCTGGAGCAGCAGAACGCGGCCCTGCGCGGGGAGCTGAGCCAACC
CCGGGCCAGGAGCCGGCGCGCCCCGACCAGCTGTGCCAGCAGGAGCTGCGCGAGCTGGCG
CAAGAGCTGGAACTTTTGGGCCGCGAGCGTGACCGGTTGCAGGTGGAGCCCGACGGGCTG
GCGGAGGACCTGGCGGCGCCTCAACCCTAGGTGGGAGGAGGAAACCCCCCAAGCGGAAGG
ACGCGGAACACAACCTTCGGGCTCTTCCGCAAGGACTTGGACAATGCCCCTCTGGCCCGC
CCGGGACTAAAACCCAAGATTGAGGCCCCTGAGGGGTGAGATTGAATTCCCTCAAGAAAC
CTGCCCAAAGGAGGACCTGCCAAACCCTGCCGTGGGATTGGTGGGAAAGCACGGCAGGTG
CCACCATGGGTGGGTGTGAGATCCACCGGGGAAACCCCGAACTGAACGGCACCCCTGGAG
GACATCCCGCGCCCCTTCCGAAACCTTCCCCGCGAAAACCTGGGCGAGGG
Restriction Sites Please inquire |
ACCN NM_006262
Insert Size 1850 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006262.3, NP_006253.2
RefSeq Size 1833 bp
RefSeq ORF 1413 bp
Locus ID 5630
UniProt ID P41219
Cytogenetics 12q13.12
Protein Pathways Amyotrophic lateral sclerosis (ALS)
Summary This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_006262" in other vectors (6)
SKU Description Size Price
RC207561 PRPH (Myc-DDK-tagged)-Human peripherin (PRPH) 10 ug
$457.00
RC207561L1 Lenti ORF clone of Human peripherin (PRPH), Myc-DDK-tagged 10 ug
$757.00
RC207561L2 Lenti ORF clone of Human peripherin (PRPH), mGFP tagged 10 ug
$757.00
RC207561L3 Lenti ORF clone of Human peripherin (PRPH), Myc-DDK-tagged 10 ug
$757.00
RC207561L4 Lenti ORF clone of Human peripherin (PRPH), mGFP tagged 10 ug
$757.00
RG207561 PRPH (tGFP-tagged) - Human peripherin (PRPH) 10 ug
$489.00 $657.00

file-alt Citations

A PRPH splice-donor variant associates with reduced sural nerve amplitude and risk of peripheral neuropathy
Bjornsdottir, Ivarsdottir, Bjarnadottir et al
Nat Commun (2019) 10 (1), 1777 DOI: 10.1038/s41467-019-09719-4
AI Summary: OriGene’s PRPH cDNA (NM_006262.3) in the pCMV6-Entry mammalian expression vector (RC207561) was used to generate plasmids for functional studies of PRPH splice variants, enabling the authors to assess the impact of the disease-associated mutation on protein structure and function.
Snippet: ormalized by sequencing depth. Generation of PRPH splice variant plasmids The full-length PRPH cDNA (NM_006262.3) in pCMV6-Entry mammalian expression vector including a Myc-DDK tag was obtained from Origene (RC207561). Plasmids for the 4 bp- and intron-retention splice variants were generated by mutating the full-length PRPH plasmid in two separated PCR reactions using Q5 site-directed mutagene
FigureFigureFigure
Showing 1-1 of 1 papers.
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