CPA5 (NM_080385) Human Tagged ORF Clone

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SKU
RC207397
CPA5 (Myc-DDK-tagged)-Human carboxypeptidase A5 (CPA5), transcript variant 1
  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CPA5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207397 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGGCACTCCTGGAGGCGGGACGCGCCCTGGGCCATCCCCCGTGGACAGGCGGACACTCCTGGTCT
TCAGCTTTATCCTGGCAGCAGCTTTGGGCCAAATGAATTTCACAGGGGACCAGGTTCTTCGAGTCCTGGC
CAAAGATGAGAAGCAGCTTTCACTTCTCGGGGATCTGGAGGGCCTGAAACCCCAGAAGGTGGACTTCTGG
CGTGGCCCAGCCAGGCCCAGCCTCCCTGTGGATATGAGAGTTCCTTTCTCTGAACTGAAAGACATCAAAG
CTTATCTGGAGTCTCATGGACTTGCTTACAGCATCATGATAAAGGACATCCAGGTGCTGCTGGATGAGGA
AAGACAGGCCATGGCGAAATCCCGCCGGCTGGAGCGCAGCACCAACAGCTTCAGTTACTCATCATACCAC
ACCCTGGAGGAGATATATAGCTGGATTGACAACTTTGTAATGGAGCATTCCGATATTGTCTCAAAAATTC
AGATTGGCAACAGCTTTGAAAACCAGTCCATTCTTGTCCTGAAGTTCAGCACTGGAGGTTCTCGGCACCC
AGCCATCTGGATTGACACTGGAATTCACTCCCGGGAGTGGATCACCCATGCCACCGGCATCTGGACTGCC
AATAAGATTGTCAGTGATTATGGCAAAGACCGTGTCCTGACAGACATACTGAATGCCATGGACATCTTCA
TAGAGCTCGTCACAAACCCTGATGGGTTTGCTTTTACCCACAGCATGAACCGCTTATGGCGGAAGAACAA
GTCCATCAGACCTGGAATCTTCTGCATCGGCGTGGATCTCAACAGGAACTGGAAGTCGGGTTTTGGAGGA
AATGGTTCTAACAGCAACCCCTGCTCAGAAACTTATCACGGGCCCTCCCCTCAGTCGGAGCCGGAGGTGG
CTGCCATAGTGAACTTCATCACAGCCCATGGCAACTTCAAGGCTCTGATCTCCATCCACAGCTACTCTCA
GATGCTTATGTACCCTTACGGCCGATCGCTGGATCCCGTTTCAAATCAGAGGGAGTTGTACGATCTTGCC
AAGGATGCGGTGGAGGCCTTGTATAAGGTCCATGGGATCGAGTACATTTTTGGCAGCATCAGCACCACCC
TCTATGTGGCCAGTGGGATCACCGTCGACTGGGCCTACGACAGTGGCATCAAGTACGCCTTCAGCTTTGA
GCTCCGGGACACTGGGCAGTATGGCTTCCTGCTGCCGGCCACACAGATCATCCCCACGGCCCAGGAGACG
TGGATGGCGCTTCGGACCATCATGGAGCACACCCTGAATCACCCCTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC207397 protein sequence
Red=Cloning site Green=Tags(s)

MQGTPGGGTRPGPSPVDRRTLLVFSFILAAALGQMNFTGDQVLRVLAKDEKQLSLLGDLEGLKPQKVDFW
RGPARPSLPVDMRVPFSELKDIKAYLESHGLAYSIMIKDIQVLLDEERQAMAKSRRLERSTNSFSYSSYH
TLEEIYSWIDNFVMEHSDIVSKIQIGNSFENQSILVLKFSTGGSRHPAIWIDTGIHSREWITHATGIWTA
NKIVSDYGKDRVLTDILNAMDIFIELVTNPDGFAFTHSMNRLWRKNKSIRPGIFCIGVDLNRNWKSGFGG
NGSNSNPCSETYHGPSPQSEPEVAAIVNFITAHGNFKALISIHSYSQMLMYPYGRSLDPVSNQRELYDLA
KDAVEALYKVHGIEYIFGSISTTLYVASGITVDWAYDSGIKYAFSFELRDTGQYGFLLPATQIIPTAQET
WMALRTIMEHTLNHPY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080385
ORF Size 1308 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080385.3
RefSeq Size 2078 bp
RefSeq ORF 1311 bp
Locus ID 93979
UniProt ID Q8WXQ8, A4D1M2
Cytogenetics 7q32.2
Protein Families Druggable Genome, Secreted Protein
MW 49 kDa
Summary Carboxypeptidases have functions ranging from digestion of food to selective biosynthesis of neuroendocrine peptides. Members of the A/B subfamily of carboxypeptidases, such as CPA5, contain an approximately 90-amino acid pro region that assists in the folding of the active carboxypeptidase domain. Cleavage of the pro region activates the enzyme (Wei et al., 2002 PubMed 11836249).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_080385" in other vectors (4)
SKU Description Size Price
RC207397L3 Lenti ORF clone of Human carboxypeptidase A5 (CPA5), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC207397L4 Lenti ORF clone of Human carboxypeptidase A5 (CPA5), transcript variant 1, mGFP tagged 10 ug
$757.00
RG207397 CPA5 (tGFP-tagged) - Human carboxypeptidase A5 (CPA5), transcript variant 1 10 ug
$489.00 $657.00
SC305781 CPA5 (untagged)-Human carboxypeptidase A5 (CPA5), transcript variant 1 10 ug
$503.00

Plasmid Map for RC207397

Download sequence for

Cloning scheme for RC207397

Circular map for RC207397

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