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CPA5 (NM_080385) Human Untagged Clone

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SKU
SC305781
CPA5 (untagged)-Human carboxypeptidase A5 (CPA5), transcript variant 1
  $503.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CPA5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC305781 representing NM_080385.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCAGGGCACCCCTGGAGGCGGGACGCGCCCTGGGCCATCCCCCGTGGACAGGCGGACACTCCTGGTC
TTCAGCTTTATCCTGGCAGCAGCTTTGGGCCAAATGAATTTCACAGGGGACCAGGTTCTTCGAGTCCTG
GCCAAAGATGAGAAGCAGCTTTCACTTCTCGGGGATCTGGAGGGCCTGAAACCCCAGAAGGTGGACTTC
TGGCGTGGCCCAGCCAGGCCCAGCCTCCCTGTGGATATGAGAGTTCCTTTCTCTGAACTGAAAGACATC
AAAGCTTATCTGGAGTCTCATGGACTTGCTTACAGCATCATGATAAAGGACATCCAGGTGCTGCTGGAT
GAGGAAAGACAGGCCATGGCGAAATCCCGCCGGCTGGAGCGCAGCACCAACAGCTTCAGTTACTCATCA
TACCACACCCTGGAGGAGATATATAGCTGGATTGACAACTTTGTAATGGAGCATTCCGATATTGTCTCA
AAAATTCAGATTGGCAACAGCTTTGAAAACCAGTCCATTCTTGTCCTGAAGTTCAGCACTGGAGGTTCT
CGGCACCCAGCCATCTGGATTGACACTGGAATTCACTCCCGGGAGTGGATCACCCATGCCACCGGCATC
TGGACTGCCAATAAGATTGTCAGTGATTATGGCAAAGACCGTGTCCTGACAGACATACTGAATGCCATG
GACATCTTCATAGAGCTCGTCACAAACCCTGATGGGTTTGCTTTTACCCACAGCATGAACCGCTTATGG
CGGAAGAACAAGTCCATCAGACCTGGAATCTTCTGCATCGGCGTGGATCTCAACAGGAACTGGAAGTCG
GGTTTTGGAGGAAATGGTTCTAACAGCAACCCCTGCTCAGAAACTTATCACGGGCCCTCCCCTCAGTCG
GAGCCGGAGGTGGCTGCCATAGTGAACTTCATCACAGCCCATGGCAACTTCAAGGCTCTGATCTCCATC
CACAGCTACTCTCAGATGCTTATGTACCCTTACGGCCGATTGCTGGAGCCCGTTTCAAATCAGAGGGAG
TTGTACGATCTTGCCAAGGATGCGGTGGAGGCCTTGTATAAGGTCCATGGGATCGAGTACATTTTTGGC
AGCATCAGCACCACCCTCTATGTGGCCAGTGGGATCACCGTCGACTGGGCCTATGACAGTGGCATCAAG
TACGCCTTCAGCTTTGAGCTCCGGGACACTGGGCAGTATGGCTTCCTGCTGCCGGCCACACAGATCATC
CCCACGGCCCAGGAGACGTGGATGGCGCTTCGGACCATCATGGAGCACACCCTGAATCACCCCTACTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_080385
Insert Size 1311 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080385.4
RefSeq Size 2078 bp
RefSeq ORF 1311 bp
Locus ID 93979
UniProt ID Q8WXQ8
Cytogenetics 7q32.2
Protein Families Druggable Genome, Secreted Protein
MW 49 kDa
Summary Carboxypeptidases have functions ranging from digestion of food to selective biosynthesis of neuroendocrine peptides. Members of the A/B subfamily of carboxypeptidases, such as CPA5, contain an approximately 90-amino acid pro region that assists in the folding of the active carboxypeptidase domain. Cleavage of the pro region activates the enzyme (Wei et al., 2002 PubMed 11836249).supplied by OMIM, Mar 2008
Transcript Variant: This variant (1) encodes isoform (1). Variants 1, 2, and 4 encode the same isoform.
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Documents
Resources
"NM_080385" in other vectors (4)
SKU Description Size Price
RC207397 CPA5 (Myc-DDK-tagged)-Human carboxypeptidase A5 (CPA5), transcript variant 1 10 ug
$457.00
RC207397L3 Lenti ORF clone of Human carboxypeptidase A5 (CPA5), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC207397L4 Lenti ORF clone of Human carboxypeptidase A5 (CPA5), transcript variant 1, mGFP tagged 10 ug
$757.00
RG207397 CPA5 (tGFP-tagged) - Human carboxypeptidase A5 (CPA5), transcript variant 1 10 ug
$489.00 $657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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