TSPAN12 (NM_012338) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TSPAN12 |
| Synonyms | EVR5; NET-2; NET2; TM4SF12 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012338 |
| ORF Size | 915 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012338.4 |
| RefSeq Size | 2579 bp |
| RefSeq ORF | 918 bp |
| Locus ID | 23554 |
| UniProt ID | O95859, A0A024R740 |
| Cytogenetics | 7q31.31 |
| Domains | transmembrane4 |
| Protein Families | Transmembrane |
| MW | 35.4 kDa |
| Summary | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012338" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206943L1 | Lenti ORF clone of Human tetraspanin 12 (TSPAN12), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC206943L2 | Lenti ORF clone of Human tetraspanin 12 (TSPAN12), mGFP tagged | 10 ug |
$600.00
|
|
| RC206943L3 | Lenti ORF clone of Human tetraspanin 12 (TSPAN12), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC206943L4 | Lenti ORF clone of Human tetraspanin 12 (TSPAN12), mGFP tagged | 10 ug |
$600.00
|
|
| RG206943 | TSPAN12 (tGFP-tagged) - Human tetraspanin 12 (TSPAN12) | 10 ug |
$489.00
$500.00
|
|
| SC112659 | TSPAN12 (untagged)-Human tetraspanin 12 (TSPAN12) | 10 ug |
$300.00
|
|
Citations
Antibody-Mediated Inhibition of Tspan12 Ameliorates Vasoproliferative Retinopathy Through Suppression of β-Catenin Signaling
Bucher, Zhang, Aguilar et al
Circulation (2017) 136 (2), 180-195 DOI: 10.1161/CIRCULATIONAHA.116.025604
Bucher, Zhang, Aguilar et al
Circulation (2017) 136 (2), 180-195 DOI: 10.1161/CIRCULATIONAHA.116.025604
AI Summary: In this study, the OriGene Tspan12 expression plasmid (#RC206943) was used to overexpress Tspan12 in 293 cells, enabling functional and mechanistic analyses of Tspan12’s role in vasoproliferative retinopathy.
Snippet: Overexpression of Tspan12 and FACS. 293 cells (#CRL-1573, ATCC) were transfected with Tspan12 (#RC206943, OriGene) for 48 h Dickinson). … Confluent 293 cells in 75 cm2 were transfected with flag-Tspan12 (RC206943, OriGene) and;Overexpression of Tspan12 and FACS. 293 cells (#CRL-1573, ATCC) were transfected with Tspan12 (#RC206943, OriGene) for 48 h Dickinson). … Confluent 293 cells in 75 cm2 were transfected with flag-Tspan12 (RC206943, OriGene) and
Novel mutations in the TSPAN12 gene in Chinese patients with familial exudative vitreoretinopathy
Xu, Huang, Li et al
Molecular Vision (2014) 20, 1296–1306
Xu, Huang, Li et al
Molecular Vision (2014) 20, 1296–1306
Vascular Endothelial-cadherin plays a role in regulating the Norrin-FZD4 pathway and can cause Familial Exududative-vitroretinopathy (FEVR)
Amasheh
Thesis (2015)
Amasheh
Thesis (2015)
Snippet: (Mississauga ,ON). Lipofectamine LTX transfection reagent was purchased from Life Technologies. Plasmids: VE-Cadherin and Tspan12 plasmids were purchased from Origene. Genes are in a pCMV6-DDK tag vector. Plasmids expressing FZD4 and LRP5 were a ... Cite SaveSaving
Showing 1-3 of 3 papers.
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