TSPAN12 (NM_012338) Human Untagged Clone

SKU SC112659

TSPAN12 (untagged)-Human tetraspanin 12 (TSPAN12)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TSPAN12
• Synonyms: EVR5; NET-2; NET2; TM4SF12
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112659 sequence for NM_012338 edited (data generated by NextGen Sequencing); ATGGCCAGAGAAGATTCCGTGAAGTGTCTGCGCTGCCTGCTCTACGCCCTCAATCTGCTC; TTTTGGTTAATGTCCATCAGTGTGTTGGCAGTTTCTGCTTGGATGAGGGACTACCTAAAT; AATGTTCTCACTTTAACTGCAGAAACGAGGGTAGAGGAAGCAGTCATTTTGACTTACTTT; CCTGTGGTTCATCCGGTCATGATTGCTGTTTGCTGTTTCCTTATCATTGTGGGGATGTTA; GGATATTGTGGAACGGTGAAAAGAAATCTGTTGCTTCTTGCATGGTACTTTGGAAGTTTG; CTTGTCATTTTCTGTGTAGAACTGGCTTGTGGCGTTTGGACATATGAACAGGAACTTATG; GTTCCAGTACAATGGTCAGATATGGTCACTTTGAAAGCCAGGATGACAAATTATGGATTA; CCTAGATATCGGTGGCTTACTCATGCTTGGAATTTTTTTCAGAGAGAGTTTAAGTGCTGT; GGAGTAGTATATTTCACTGACTGGTTGGAAATGACAGAGATGGACTGGCCCCCAGATTCC; TGCTGTGTTAGAGAATTCCCAGGATGTTCCAAACAGGCCCACCAGGAAGATCTCAGTGAC; CTTTATCAAGAGGGTTGTGGGAAGAAAATGTATTCCTTTTTGAGAGGAACCAAACAACTG; CAGGTGCTGAGGTTTCTGGGAATCTCCATTGGGGTGACACAAATCCTGGCCATGATTCTC; ACCATTACTCTGCTCTGGGCTCTGTATTATGATAGAAGGGAGCCTGGGACAGACCAAATG; ATGTCCTTGAAGAATGACAACTCTCAGCACCTGTCATGTCCCTCAGTAGAACTGTTGAAA; CCAAGCCTGTCAAGAATCTTTGAACACACATCCATGGCAAACAGCTTTAATACACACTTT; GAGATGGAGGAGTTATAA; ; Clone variation with respect to NM_012338.3; 765 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012338 unedited; AAACCCAATTCGGCACGAGGGGCTCACAATGGCCAGAGAAGATTCCGTGAAGTGTCTGCG; CTGCCTGCTCTACGCCCTCAATCTGCTCTTTTGGTTAATGTCCATCAGTGTGTTGGCAGT; TTCTGCTTGGATGAGGGACTACCTAAATAATGTTCTCACTTTAACTGCAGAAACGAGGGT; ATTATAAGCAGTCATTTTGACTTACTTTCCTGTGGTTCATCCGGTCATGATTGCTGTTTG; CTGTTTCCTTATCATTGTGGGGATGTTAGGATATTGTGGAACGGTGAAAAGAAATCTGTT; GCTTCTTGCATGGTACTTTGGAAGTTTGCTTGTCATTTTCTGTGTAGAACTGGCTTGTGG; CGTTTGGACATATGAACAGGAACTTATGGTTCCAGTACAATGGTCAGATATGGTCACTTT; GAAAGCCAGGATGACAAATTATGGATTACCTAGATATCGGTGGCTTACTCATGCTTGGAA; TTTTTTTCAGAGAGAGTTTAAGTGCTGTGGAGTAGTATATTTCACTGACTGGTTGGAAAT; GACAGAGATGGACTGGCCCCCAGATTCCTGCTGTGTTAGAGAATTCCCAGGATGTTCCAA; ACAGGCCCACCAGGAAGATCTCAGTGACCTTTATCAAGAGGGTTGTGGGAAGAAAATGTA; TTCCTTTTTGAGAGGAACCAAAACACTGCANGTGCTGAGGTTTCTGGGGATCTCCATTTG; GGTGACACANATCCTGNCCATGATTCTCACCATTACTCTGCTCTGGGCTCTGTATTATGA; TAGAAGGGAGCCTTGGACAAGACAAATGATGTCCTTGAAGAAATGACACTCTCAGCACCT; GTCATGTCCCTCAGTAGAACTGGTGAAAACCAGCCCTGTCAGAAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012338 unedited; GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACTCTCTA; AAAAAATTTATTGACACATTTTAATTTTCTTTCCATATTCTGAGAAATAAACAAAATACA; CAAAATACAGTAAAATGCACTTTTCCCATTTCAAATAAATATACTATACTCTTGTAAAAA; AATTTAATAATCTGGGTAATTTGCCACAGTATATTAATCAGTGAAATAAAACAAAGAATC; TCTAAATTGTCACATGTTTTAATGATTCTCACAAGCATTTTTCTGGAAAAAAAGAACACT; ATTTTCTGTAAAATTTATATTGACTTGGAGACTATTGCTTCTTGTTTGGAGAAGGCAAAG; ATAAGCTTTTTAAAAAGTGTTTAGTGTCAAAAAGAGTCTTCATTTGGGAGAAAAACAGCA; TACAAGTCCTTTCTGAATCAACTAAGTTATGTGCAAATTTTCTTATTAATACTTCCTAAA; GACCAGACTACATTCTGAATAATGATTCCTATGTATCACACTGGTAATTTTCAAAATTAG; TGGTTAAGTATTTAAATAACAGTTATATGTGTAATATAAGCCCAGGACAGAATAGTAAAG; CTTTATTAACAGGAAAGGCTAAAAATAATGATGCTATACAGGACAAATTTTCCTTTTCAT; AATGGTTATTCTTAACATTATCAGAACTGAAATCGACTGAATTATACAGATTTAACACAG; ACTTATATACAAAAAATCCCTGATCAGCTTAAGACATGTGNTAGCTTGCTCTTTTAAGTG; GGTAGATATTTTAGTACCAGCAAAATACATTTAGGTAGTCCCATATTGAAAGTTTCTGAT; TATAATTATTTAGCTCAAAGAATAGACGCTGAGTATAAAAGTATTAGATTTCTACTCCTA; TGTACATTAATAGACTTATAACTAGTGGAGAGTAATCACCTTATATGCTCAGCCACATTG; TGACTGTTGATGAGCGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_012338
• Insert Size: 2090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012338.3, NP_036470.1
• RefSeq Size: 2579 bp
• RefSeq ORF: 918 bp
• Locus ID: 23554
• UniProt ID: O95859
• Cytogenetics: 7q31.31
• Domains: transmembrane4
• Protein Families: Transmembrane
• Summary: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. [provided by RefSeq, Jul 2008]