IFIT5 (NM_012420) Human Tagged ORF Clone

SKU RC206499

IFIT5 (Myc-DDK-tagged)-Human interferon-induced protein with tetratricopeptide repeats 5 (IFIT5)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: IFIT5
• Synonyms: ISG58; P58; RI58
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206499 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGTGAAATTCGTAAGGACACCTTGAAGGCCATTCTGTTGGAGTTAGAATGTCATTTTACATGGAATT; TACTTAAGGAAGACATTGATCTGTTTGAGGTAGAAGATACAATTGGGCAACAGCTTGAATTTCTTACCAC; AAAATCTAGACTTGCTCTTTATAACCTATTGGCCTATGTGAAACACCTAAAAGGCCAAAATAAAGACGCC; CTTGAGTGCTTGGAACAAGCAGAAGAAATAATCCAGCAAGAACACTCAGACAAAGAAGAAGTACGAAGCC; TGGTCACTTGGGGAAACTATGCCTGGGTGTATTATCACATGGACCAGCTTGAAGAAGCTCAGAAGTATAC; AGGTAAGATAGGGAATGTCTGTAAGAAATTGTCCAGTCCTTCTAACTACAAGTTGGAGTGTCCTGAGACT; GACTGTGAGAAAGGCTGGGCACTCTTGAAATTTGGAGGAAAGTATTATCAAAAGGCTAAAGCGGCTTTTG; AGAAGGCTCTGGAAGTGGAGCCTGACAATCCAGAATTTAACATCGGCTATGCTATCACAGTGTATCGGCT; GGATGATTCTGATAGAGAAGGGTCTGTAAAGAGCTTTTCTCTGGGGCCTTTGAGAAAGGCTGTTACCCTG; AACCCAGATAACAGCTATATTAAGGTTTTTCTGGCACTGAAGCTTCAAGATGTACATGCAGAAGCTGAAG; GGGAAAAGTATATTGAAGAAATCCTGGACCAAATATCATCCCAGCCTTACGTCCTTCGTTATGCAGCCAA; GTTCTATAGGAGAAAAAATTCCTGGAACAAAGCTCTCGAACTTTTAAAAAAGGCCTTGGAGGTGACACCA; ACTTCTTCTTTCCTGCATCACCAGATGGGACTTTGCTACAGGGCACAAATGATCCAAATCAAGAAGGCCA; CACACAACAGACCTAAAGGAAAGGATAAACTAAAGGTTGATGAGCTGATTTCATCTGCTATATTTCATTT; CAAAGCAGCCATGGAACGAGACTCTATGTTTGCATTTGCCTACACAGACCTGGCCAACATGTATGCTGAA; GGAGGCCAGTATAGCAATGCTGAGGACATTTTCCGGAAAGCTCTTCGTCTGGAGAACATAACCGATGATC; ACAAACATCAGATCCATTACCACTATGGCCGCTTTCAGGAATTTCACCGTAAATCAGAAAATACTGCCAT; CCATCATTATTTAGAAGCCTTAAAGGTCAAAGACAGATCACCCCTTCGCACCAAACTGACAAGTGCTCTG; AAGAAATTGTCTACCAAGAGACTTTGTCACAATGCTTTAGATGTGCAGAGTTTAAGTGCCCTAGGGTTTG; TTTACAAGCTGGAAGGAGAAAAGAGGCAAGCTGCTGAGTACTATGAGAAGGCACAAAAGATAGATCCAGA; AAATGCAGAATTCCTGACTGCTCTCTGTGAGCTCCGACTTTCCATT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206499 protein sequence; Red=Cloning site Green=Tags(s); ; MSEIRKDTLKAILLELECHFTWNLLKEDIDLFEVEDTIGQQLEFLTTKSRLALYNLLAYVKHLKGQNKDA; LECLEQAEEIIQQEHSDKEEVRSLVTWGNYAWVYYHMDQLEEAQKYTGKIGNVCKKLSSPSNYKLECPET; DCEKGWALLKFGGKYYQKAKAAFEKALEVEPDNPEFNIGYAITVYRLDDSDREGSVKSFSLGPLRKAVTL; NPDNSYIKVFLALKLQDVHAEAEGEKYIEEILDQISSQPYVLRYAAKFYRRKNSWNKALELLKKALEVTP; TSSFLHHQMGLCYRAQMIQIKKATHNRPKGKDKLKVDELISSAIFHFKAAMERDSMFAFAYTDLANMYAE; GGQYSNAEDIFRKALRLENITDDHKHQIHYHYGRFQEFHRKSENTAIHHYLEALKVKDRSPLRTKLTSAL; KKLSTKRLCHNALDVQSLSALGFVYKLEGEKRQAAEYYEKAQKIDPENAEFLTALCELRLSI; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012420
• ORF Size: 1446 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012420.1, NP_036552.1
• RefSeq Size: 4034 bp
• RefSeq ORF: 1449 bp
• Locus ID: 24138
• UniProt ID: Q13325
• Cytogenetics: 10q23.31
• Domains: TPR
• MW: 55.8 kDa
• Summary: Interferon-induced RNA-binding protein involved in the human innate immune response. Has a broad and adaptable RNA structure recognition important for RNA recognition specificity in antiviral defense. Binds precursor and processed tRNAs as well as poly-U-tailed tRNA fragments (PubMed:25092312, PubMed:23317505, PubMed:23774268). Specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner (PubMed:23334420). Also recognizes and selectively binds AT-rich dsDNA (PubMed:23774268). Additionally, as a mediator in innate immunity, regulates positively IKK-NFKB signaling by sinergizing the recruitment of IKK to MAP3K7 (PubMed:26334375).[UniProtKB/Swiss-Prot Function]