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IFIT5 (NM_012420) Human Untagged Clone

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SKU
SC111218
IFIT5 (untagged)-Human interferon-induced protein with tetratricopeptide repeats 5 (IFIT5)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol IFIT5
Synonyms ISG58; P58; RI58
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC111218 sequence for NM_012420 edited (data generated by NextGen Sequencing)
ATGAGTGAAATTCGTAAGGACACCTTGAAGGCCATTCTGTTGGAGTTAGAATGTCATTTT
ACATGGAATTTACTTAAGGAAGACATTGATCTGTTTGAGGTAGAAGATACAATTGGGCAA
CAGCTTGAATTTCTTACCACAAAATCTAGACTTGCTCTTTATAACCTATTGGCCTATGTG
AAACACCTAAAAGGCCAAAATAAAGACGCCCTTGAGTGCTTGGAACAAGCAGAAGAAATA
ATCCAGCAAGAACACTCAGACAAAGAAGAAGTACGAAGCCTGGTCACTTGGGGAAACTAT
GCCTGGGTGTATTATCACATGGACCAGCTTGAAGAAGCTCAGAAGTATACAGGTAAGATA
GGGAATGTCTGTAAGAAATTGTCCAGTCCTTCTAACTACAAGTTGGAGTGTCCTGAGACT
GACTGTGAGAAAGGCTGGGCACTCTTGAAATTTGGAGGAAAGTATTATCAAAAGGCTAAA
GCGGCTTTTGAGAAGGCTCTGGAAGTGGAGCCTGACAATCCAGAATTTAACATCGGCTAT
GCTATCACAGTGTATCGGCTGGATGATTCTGATAGAGAAGGGTCTGTAAAGAGCTTTTCT
CTGGGGCCTTTGAGAAAGGCTGTTACCCTGAACCCAGATAACAGCTATATTAAGGTTTTT
CTGGCACTGAAGCTTCAAGATGTACATGCAGAAGCTGAAGGGGAAAAGTATATTGAAGAA
ATCCTGGACCAAATATCATCCCAGCCTTACGTCCTTCGTTATGCAGCCAAGTTCTATAGG
AGAAAAAATTCCTGGAACAAAGCTCTCGAACTTTTAAAAAAGGCCTTGGAGGTGACACCA
ACTTCTTCTTTCCTGCATCACCAGATGGGACTTTGCTACAGGGCACAAATGATCCAAATC
AAGAAGGCCACACACAACAGACCTAAAGGAAAGGATAAACTAAAGGTTGATGAGCTGATT
TCATCTGCTATATTTCATTTCAAAGCAGCCATGGAACGAGACTCTATGTTTGCATTTGCC
TACACAGACCTGGCCAACATGTACGCTGAAGGAGGCCAGTATAGCAATGCTGAGGACATT
TTCCGGAAAGCTCTTCGTCTGGAGAACATAACCGATGATCACAAACATCAGATCCATTAC
CACTATGGCCGCTTTCAGGAATTTCACCGTAAATCAGAAAATACTGCCATCCATCATTAT
TTAGAAGCCTTAAAGGTCAAAGACAGATCACCCCTTCGCACCAAACTGACAAGTGCTCTG
AAGAAATTGTCTACCAAGAGACTTTGTCACAATGCTTTAGATGTGCAGAGTTTAAGTGCC
CTAGGGTTTGTTTACAAGCTGGAAGGAGAAAAGAGGCAAGCTGCTGAGTACTATGAGAAG
GCACAAAAGATAGATCCAGAAAATGCAGAATTCCTGACTGCTCTCTGTGAGCTCCGACTT
TCCATTTAA

Clone variation with respect to NM_012420.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_012420 unedited
TACGACTCCTATGGGGCGGCCGCGATTCGGCACGAGGATTCAATCTCCAGTTTCCTGTTC
TTGCTGGGGCTGGGGTCTCTCCTTTAACAAAGACACGCCGCGCGGCCGAGTCCAGGGGCT
GCAGAGGCCTGGCGCGCGCACGCGCACGCGCACGCCCACCGCGCGGCTTCCCGCGGTCCC
CGGTGCTGAGGAGAGAGCGATCCGAGGGACTGCGCCGCCCGGACGGCCTGCAGAGCGCTG
CCATCATGAGTGAAATTCGTAAGGACACCTTGAAGGCCATTCTGTTGGAGTTAGAATGTC
ATTTTACATGGAATTTACTTAAGGAAGACATTGATCTGTTTGAGGTAGAAGATACAATTG
GGCAACAGCTTGAATTTCTTACCACAAAATCTAGACTTGCTCTTTATAACCTATTGGCCT
ATGTGAAACACCTAAAAGGCCAAAATAAAGACGCCCTTGAGTGCTTGGAACAAGCAGAAG
AAATAATCCAGCAAGAACACTCAGACAAAGAAGAAGTACGAAGCCTGGTCACTTGGGGAA
ACTATGCCTGGGTGTATTATCACATGGACCAGCTTGAAGAAGCTCAGAAGTATACAGGTA
AGATAGGGAATGTCTGTAAGAAATTGTCCAGTCCTTCTAACNTACAAGTTGGAGTGTCCT
GAGACTGACTGTGAGAAAGGGCTGGGCACTCTTGAAATTTGGAGGAAAGTATTATCAAAA
GGCTAAAGCGGCTNTTGAGAAGCTCTGGNAGTGGAGCCTGACATCCAGATNTACATCGGC
TTGCTATCACAGTGTATCGGCTGGATGATTCTGATAGAGAGGGTCTGTAAGAGCTTTCTC
TGGGGCCTTGAGAAGGCTGGTACCTGACCAGATACAGTTANTTAGGTTTTCTGCCTGAGC
TTCAAAGTACTGCANACTGAGGGGAAAGTTATGAGAATCTGACAATTCATCCAACTTG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_012420 unedited
TTTTTTTTTTTTTTTTTAAGTAAAACAAAGNATTTTAAAAATTAATACGTGAGAAACACT
GCAGTATATATGTATGTCTGATTACGCATAGAAAAAAATCTGGAAGTTTACATATCAAAT
TATAAGTTATAAGTTTACCTTGAGGGTGTGGAACTGTGGTAGGGTGAGAGGACTGCTCTC
ATTTTTAAATTCACATACTTTTGTAATTTTTTTTTGAAATTTACAGTGAGAATTCATTAT
TTTTATAATAATCAAACCATAATAGAAATTTTAAAGATGATAAAAAACAAAAAGTAAAAC
ACACACACATACATAATAATTTTAAGAGTTACATTGGAGGAATGGAGAATTAAATCACAT
GGTACTTTTCCACTAATACTGTGATGGTACATAATTGTGTAGTCGGTAATCAAAGAAACT
ACATTAGAGAAGCCACATTAAGTAACATCCGAATTAAAAAGTCCCCTGGGATAAGAGCCC
TCAAGCAGCGACCCCTCAAGGTGGATATTAAAACTGAAAGCAGTGCTTAACTCTTCCTGG
AGTATCTACATGAAAAAAACAACATTCTGAAAATTTCACATTAAAAAAAAAGCTTCATAG
AAGTCACTTTGAAATGCTAAAATAGCCAAAGGCCAGAAATTGAGGGTATAAAAACTCCAT
T
Restriction Sites NotI-NotI |
ACCN NM_012420
Insert Size 3410 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012420.1, NP_036552.1
RefSeq Size 3906 bp
RefSeq ORF 1449 bp
Locus ID 24138
UniProt ID Q13325
Cytogenetics 10q23.31
Domains TPR
Summary Interferon-induced RNA-binding protein involved in the human innate immune response. Has a broad and adaptable RNA structure recognition important for RNA recognition specificity in antiviral defense. Binds precursor and processed tRNAs as well as poly-U-tailed tRNA fragments (PubMed:25092312, PubMed:23317505, PubMed:23774268). Specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner (PubMed:23334420). Also recognizes and selectively binds AT-rich dsDNA (PubMed:23774268). Additionally, as a mediator in innate immunity, regulates positively IKK-NFKB signaling by sinergizing the recruitment of IKK to MAP3K7 (PubMed:26334375).UniProtKB/Swiss-Prot Function
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"NM_012420" in other vectors (6)
SKU Description Size Price
RC206499 IFIT5 (Myc-DDK-tagged)-Human interferon-induced protein with tetratricopeptide repeats 5 (IFIT5) 10 ug
$457.00
RC206499L1 Lenti ORF clone of Human interferon-induced protein with tetratricopeptide repeats 5 (IFIT5), Myc-DDK-tagged 10 ug
$757.00
RC206499L2 Lenti ORF clone of Human interferon-induced protein with tetratricopeptide repeats 5 (IFIT5), mGFP tagged 10 ug
$757.00
RC206499L3 Lenti ORF clone of Human interferon-induced protein with tetratricopeptide repeats 5 (IFIT5), Myc-DDK-tagged 10 ug
$757.00
RC206499L4 Lenti ORF clone of Human interferon-induced protein with tetratricopeptide repeats 5 (IFIT5), mGFP tagged 10 ug
$757.00
RG206499 IFIT5 (tGFP-tagged) - Human interferon-induced protein with tetratricopeptide repeats 5 (IFIT5) 10 ug
$489.00 $657.00

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