IFIT5 (NM_012420) Human Untagged Clone

SKU SC111218

IFIT5 (untagged)-Human interferon-induced protein with tetratricopeptide repeats 5 (IFIT5)

Product Data

• Type: Human Untagged Clone
• Target Symbol: IFIT5
• Synonyms: ISG58; P58; RI58
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111218 sequence for NM_012420 edited (data generated by NextGen Sequencing); ATGAGTGAAATTCGTAAGGACACCTTGAAGGCCATTCTGTTGGAGTTAGAATGTCATTTT; ACATGGAATTTACTTAAGGAAGACATTGATCTGTTTGAGGTAGAAGATACAATTGGGCAA; CAGCTTGAATTTCTTACCACAAAATCTAGACTTGCTCTTTATAACCTATTGGCCTATGTG; AAACACCTAAAAGGCCAAAATAAAGACGCCCTTGAGTGCTTGGAACAAGCAGAAGAAATA; ATCCAGCAAGAACACTCAGACAAAGAAGAAGTACGAAGCCTGGTCACTTGGGGAAACTAT; GCCTGGGTGTATTATCACATGGACCAGCTTGAAGAAGCTCAGAAGTATACAGGTAAGATA; GGGAATGTCTGTAAGAAATTGTCCAGTCCTTCTAACTACAAGTTGGAGTGTCCTGAGACT; GACTGTGAGAAAGGCTGGGCACTCTTGAAATTTGGAGGAAAGTATTATCAAAAGGCTAAA; GCGGCTTTTGAGAAGGCTCTGGAAGTGGAGCCTGACAATCCAGAATTTAACATCGGCTAT; GCTATCACAGTGTATCGGCTGGATGATTCTGATAGAGAAGGGTCTGTAAAGAGCTTTTCT; CTGGGGCCTTTGAGAAAGGCTGTTACCCTGAACCCAGATAACAGCTATATTAAGGTTTTT; CTGGCACTGAAGCTTCAAGATGTACATGCAGAAGCTGAAGGGGAAAAGTATATTGAAGAA; ATCCTGGACCAAATATCATCCCAGCCTTACGTCCTTCGTTATGCAGCCAAGTTCTATAGG; AGAAAAAATTCCTGGAACAAAGCTCTCGAACTTTTAAAAAAGGCCTTGGAGGTGACACCA; ACTTCTTCTTTCCTGCATCACCAGATGGGACTTTGCTACAGGGCACAAATGATCCAAATC; AAGAAGGCCACACACAACAGACCTAAAGGAAAGGATAAACTAAAGGTTGATGAGCTGATT; TCATCTGCTATATTTCATTTCAAAGCAGCCATGGAACGAGACTCTATGTTTGCATTTGCC; TACACAGACCTGGCCAACATGTACGCTGAAGGAGGCCAGTATAGCAATGCTGAGGACATT; TTCCGGAAAGCTCTTCGTCTGGAGAACATAACCGATGATCACAAACATCAGATCCATTAC; CACTATGGCCGCTTTCAGGAATTTCACCGTAAATCAGAAAATACTGCCATCCATCATTAT; TTAGAAGCCTTAAAGGTCAAAGACAGATCACCCCTTCGCACCAAACTGACAAGTGCTCTG; AAGAAATTGTCTACCAAGAGACTTTGTCACAATGCTTTAGATGTGCAGAGTTTAAGTGCC; CTAGGGTTTGTTTACAAGCTGGAAGGAGAAAAGAGGCAAGCTGCTGAGTACTATGAGAAG; GCACAAAAGATAGATCCAGAAAATGCAGAATTCCTGACTGCTCTCTGTGAGCTCCGACTT; TCCATTTAA; ; Clone variation with respect to NM_012420.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012420 unedited; TACGACTCCTATGGGGCGGCCGCGATTCGGCACGAGGATTCAATCTCCAGTTTCCTGTTC; TTGCTGGGGCTGGGGTCTCTCCTTTAACAAAGACACGCCGCGCGGCCGAGTCCAGGGGCT; GCAGAGGCCTGGCGCGCGCACGCGCACGCGCACGCCCACCGCGCGGCTTCCCGCGGTCCC; CGGTGCTGAGGAGAGAGCGATCCGAGGGACTGCGCCGCCCGGACGGCCTGCAGAGCGCTG; CCATCATGAGTGAAATTCGTAAGGACACCTTGAAGGCCATTCTGTTGGAGTTAGAATGTC; ATTTTACATGGAATTTACTTAAGGAAGACATTGATCTGTTTGAGGTAGAAGATACAATTG; GGCAACAGCTTGAATTTCTTACCACAAAATCTAGACTTGCTCTTTATAACCTATTGGCCT; ATGTGAAACACCTAAAAGGCCAAAATAAAGACGCCCTTGAGTGCTTGGAACAAGCAGAAG; AAATAATCCAGCAAGAACACTCAGACAAAGAAGAAGTACGAAGCCTGGTCACTTGGGGAA; ACTATGCCTGGGTGTATTATCACATGGACCAGCTTGAAGAAGCTCAGAAGTATACAGGTA; AGATAGGGAATGTCTGTAAGAAATTGTCCAGTCCTTCTAACNTACAAGTTGGAGTGTCCT; GAGACTGACTGTGAGAAAGGGCTGGGCACTCTTGAAATTTGGAGGAAAGTATTATCAAAA; GGCTAAAGCGGCTNTTGAGAAGCTCTGGNAGTGGAGCCTGACATCCAGATNTACATCGGC; TTGCTATCACAGTGTATCGGCTGGATGATTCTGATAGAGAGGGTCTGTAAGAGCTTTCTC; TGGGGCCTTGAGAAGGCTGGTACCTGACCAGATACAGTTANTTAGGTTTTCTGCCTGAGC; TTCAAAGTACTGCANACTGAGGGGAAAGTTATGAGAATCTGACAATTCATCCAACTTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012420 unedited; TTTTTTTTTTTTTTTTTAAGTAAAACAAAGNATTTTAAAAATTAATACGTGAGAAACACT; GCAGTATATATGTATGTCTGATTACGCATAGAAAAAAATCTGGAAGTTTACATATCAAAT; TATAAGTTATAAGTTTACCTTGAGGGTGTGGAACTGTGGTAGGGTGAGAGGACTGCTCTC; ATTTTTAAATTCACATACTTTTGTAATTTTTTTTTGAAATTTACAGTGAGAATTCATTAT; TTTTATAATAATCAAACCATAATAGAAATTTTAAAGATGATAAAAAACAAAAAGTAAAAC; ACACACACATACATAATAATTTTAAGAGTTACATTGGAGGAATGGAGAATTAAATCACAT; GGTACTTTTCCACTAATACTGTGATGGTACATAATTGTGTAGTCGGTAATCAAAGAAACT; ACATTAGAGAAGCCACATTAAGTAACATCCGAATTAAAAAGTCCCCTGGGATAAGAGCCC; TCAAGCAGCGACCCCTCAAGGTGGATATTAAAACTGAAAGCAGTGCTTAACTCTTCCTGG; AGTATCTACATGAAAAAAACAACATTCTGAAAATTTCACATTAAAAAAAAAGCTTCATAG; AAGTCACTTTGAAATGCTAAAATAGCCAAAGGCCAGAAATTGAGGGTATAAAAACTCCAT; T;
• Restriction Sites: NotI-NotI
• ACCN: NM_012420
• Insert Size: 3410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012420.1, NP_036552.1
• RefSeq Size: 3906 bp
• RefSeq ORF: 1449 bp
• Locus ID: 24138
• UniProt ID: Q13325
• Cytogenetics: 10q23.31
• Domains: TPR
• Summary: Interferon-induced RNA-binding protein involved in the human innate immune response. Has a broad and adaptable RNA structure recognition important for RNA recognition specificity in antiviral defense. Binds precursor and processed tRNAs as well as poly-U-tailed tRNA fragments (PubMed:25092312, PubMed:23317505, PubMed:23774268). Specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner (PubMed:23334420). Also recognizes and selectively binds AT-rich dsDNA (PubMed:23774268). Additionally, as a mediator in innate immunity, regulates positively IKK-NFKB signaling by sinergizing the recruitment of IKK to MAP3K7 (PubMed:26334375).[UniProtKB/Swiss-Prot Function]