SLC19A3 (NM_025243) Human Tagged ORF Clone

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SKU
RC206409
SLC19A3 (Myc-DDK-tagged)-Human solute carrier family 19, member 3 (SLC19A3)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC19A3
Synonyms BBGD; THMD2; thTr-2; THTR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206409 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATTGTTACAGAACTTCACTAAGCAGTTCCTGGATTTACCCCACTGTGATCCTCTGCTTATTTGGTT
TTTTCTCCATGATGAGACCCTCAGAACCGTTCCTTATCCCATATTTATCTGGACCAGATAAAAACCTGAC
CAGTGCAGAGATAACAAATGAGATCTTCCCCGTTTGGACATACTCCTACCTGGTGCTGCTGCTGCCTGTG
TTTGTCCTCACCGATTATGTCCGCTACAAGCCAGTCATCATCTTGCAAGGTATCAGTTTCATCATTACCT
GGCTGCTGCTGTTGTTTGGCCAAGGAGTGAAGACCATGCAGGTTGTAGAGTTCTTCTATGGGATGGTCAC
CGCCGCCGAGGTGGCCTACTACGCCTACATATACAGCGTGGTCAGCCCCGAGCACTACCAGAGAGTGAGC
GGCTACTGCAGGAGCGTCACGCTGGCCGCCTACACAGCAGGGTCGGTGCTGGCTCAACTCTTGGTATCCC
TGGCGAACATGTCGTACTTTTACCTCAACGTCATATCCTTGGCCTCTGTCTCCGTGGCTTTCCTTTTCTC
ACTTTTCCTACCAATGCCCAAGAAAAGCATGTTTTTTCATGCAAAACCCAGCAGAGAAATAAAGAAGTCA
TCAAGCGTGAATCCAGTATTAGAGGAAACTCACGAAGGTGAAGCACCAGGCTGTGAAGAGCAGAAACCCA
CATCAGAAATACTCAGCACTTCAGGGAAGCTGAATAAGGGCCAGCTGAACAGCCTGAAACCAAGCAATGT
GACTGTGGACGTTTTTGTGCAGTGGTTCCAAGATTTGAAGGAGTGCTACTCCTCAAAACGTCTTTTCTAC
TGGTCTCTATGGTGGGCTTTCGCCACAGCAGGTTTTAACCAGGTTTTGAACTATGTTCAAATCCTGTGGG
ATTACAAGGCGCCATCCCAAGATTCTTCCATCTATAATGGGGCCGTAGAAGCTATTGCAACCTTTGGAGG
GGCTGTGGCTGCCTTTGCAGTGGGTTATGTGAAAGTCAACTGGGACCTTCTGGGAGAGCTGGCTCTGGTG
GTCTTCTCAGTTGTCAATGCCGGTTCTTTATTTCTCATGCATTACACAGCCAATATCTGGGCGTGCTATG
CTGGCTATTTGATATTCAAGTCCAGCTATATGCTTCTTATAACCATAGCAGTATTTCAGATTGCAGTTAA
TCTGAATGTGGAACGCTATGCCTTGGTATTTGGAATCAACACCTTTATTGCCTTGGTGATTCAGACCATC
ATGACTGTGATTGTAGTAGATCAGAGAGGGCTCAACTTGCCAGTCAGCATTCAGTTTTTAGTTTATGGGA
GCTATTTTGCAGTAATTGCTGGAATTTTCCTAATGAGAAGCATGTATATTACCTACTCAACCAAATCCCA
GAAGGATGTACAGAGCCCTGCTCCAAGTGAGAATCCAGATGTGTCTCACCCAGAGGAAGAGAGTAATATC
ATCATGTCAACAAAACTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206409 protein sequence
Red=Cloning site Green=Tags(s)

MDCYRTSLSSSWIYPTVILCLFGFFSMMRPSEPFLIPYLSGPDKNLTSAEITNEIFPVWTYSYLVLLLPV
FVLTDYVRYKPVIILQGISFIITWLLLLFGQGVKTMQVVEFFYGMVTAAEVAYYAYIYSVVSPEHYQRVS
GYCRSVTLAAYTAGSVLAQLLVSLANMSYFYLNVISLASVSVAFLFSLFLPMPKKSMFFHAKPSREIKKS
SSVNPVLEETHEGEAPGCEEQKPTSEILSTSGKLNKGQLNSLKPSNVTVDVFVQWFQDLKECYSSKRLFY
WSLWWAFATAGFNQVLNYVQILWDYKAPSQDSSIYNGAVEAIATFGGAVAAFAVGYVKVNWDLLGELALV
VFSVVNAGSLFLMHYTANIWACYAGYLIFKSSYMLLITIAVFQIAVNLNVERYALVFGINTFIALVIQTI
MTVIVVDQRGLNLPVSIQFLVYGSYFAVIAGIFLMRSMYITYSTKSQKDVQSPAPSENPDVSHPEEESNI
IMSTKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025243
ORF Size 1488 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025243.4
RefSeq Size 3775 bp
RefSeq ORF 1491 bp
Locus ID 80704
UniProt ID Q9BZV2
Cytogenetics 2q36.3
Domains Folate_carrier
Protein Families Transmembrane
MW 55.7 kDa
Summary This gene encodes a ubiquitously expressed transmembrane thiamine transporter that lacks folate transport activity. Mutations in this gene cause biotin-responsive basal ganglia disease (BBGD); a recessive disorder manifested in childhood that progresses to chronic encephalopathy, dystonia, quadriparesis, and death if untreated. Patients with BBGD have bilateral necrosis in the head of the caudate nucleus and in the putamen. Administration of high doses of biotin in the early progression of the disorder eliminates pathological symptoms while delayed treatment results in residual paraparesis, mild cognitive disability, or dystonia. Administration of thiamine is ineffective in the treatment of this disorder. Experiments have failed to show that this protein can transport biotin. Mutations in this gene also cause a Wernicke's-like encephalopathy.provided by RefSeq, Jan 2010
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Documents
Resources
"NM_025243" in other vectors (5)
SKU Description Size Price
RC206409L3 Lenti ORF clone of Human solute carrier family 19, member 3 (SLC19A3), Myc-DDK-tagged 10 ug
$757.00
RC206409L4 Lenti ORF clone of Human solute carrier family 19, member 3 (SLC19A3), mGFP tagged 10 ug
$757.00
RG206409 SLC19A3 (tGFP-tagged) - Human solute carrier family 19, member 3 (SLC19A3) 10 ug
$489.00 $657.00
SC108121 SLC19A3 (untagged)-Human solute carrier family 19, member 3 (SLC19A3) 10 ug
$457.00
SC324186 SLC19A3 (untagged)-Human solute carrier family 19, member 3 (SLC19A3) 10 ug
$457.00

Plasmid Map for RC206409

Download sequence for

Cloning scheme for RC206409

Circular map for RC206409

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