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DUSP19 (NM_080876) Human Tagged ORF Clone

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SKU
RC206095
DUSP19 (Myc-DDK-tagged)-Human dual specificity phosphatase 19 (DUSP19), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DUSP19
Synonyms DUSP17; LMWDSP3; SKRP1; TS-DSP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206095 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACTCCCTTAACCAGGAAATTAAAGCATTCTCCCGGAATAATCTCAGGAAGCAATGCACCAGGGTGA
CAACGCTAACTGGAAAGAAAATTATAGAAACATGGAAAGATGCCAGAATTCATGTTGTGGAAGAAGTAGA
GCCGAGCAGTGGGGGTGGTTGTGGTTATGTGCAGGACCTTAGCTCGGACCTGCAAGTTGGCGTTATTAAG
CCATGGTTGCTCCTAGGGTCACAAGATGCTGCTCATGATTTGGATACACTGAAAAAGAATAAGGTGACTC
ATATTCTTAATGTTGCATATGGAGTTGAAAATGCTTTCCTCAGTGACTTTACATATAAGAGCATTTCTAT
ATTGGATCTGCCTGAAACCAACATCCTGTCTTATTTTCCAGAATGTTTTGAATTTATTGAAGAAGCAAAA
AGAAAAGATGGAGTGGTTCTTGTTCATTGTAATGCAGGCGTTTCCAGGGCTGCTGCAATTGTAATAGGTT
TCCTGATGAATTCTGAACAAACCTCATTTACCAGTGCTTTTTCTTTGGTGAAAAATGCAAGACCTTCCAT
ATGTCCAAATTCTGGCTTCATGGAGCAGCTTCGTACATATCAAGAGGGCAAAGAAAGCAATAAGTGTGAC
AGAATACAGGAGAACAGTTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206095 protein sequence
Red=Cloning site Green=Tags(s)

MYSLNQEIKAFSRNNLRKQCTRVTTLTGKKIIETWKDARIHVVEEVEPSSGGGCGYVQDLSSDLQVGVIK
PWLLLGSQDAAHDLDTLKKNKVTHILNVAYGVENAFLSDFTYKSISILDLPETNILSYFPECFEFIEEAK
RKDGVVLVHCNAGVSRAAAIVIGFLMNSEQTSFTSAFSLVKNARPSICPNSGFMEQLRTYQEGKESNKCD
RIQENSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080876
ORF Size 651 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080876.4
RefSeq Size 5379 bp
RefSeq ORF 654 bp
Locus ID 142679
UniProt ID Q8WTR2
Cytogenetics 2q32.1
Protein Families Druggable Genome, Phosphatase
MW 24.2 kDa
Summary Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. They have been implicated as major modulators of critical signaling pathways. DUSP19 contains a variation of the consensus DUSP C-terminal catalytic domain, with the last serine residue replaced by alanine, and lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase phosphatase) class of DUSPs (see MIM 600714) (summary by Patterson et al., 2009 PubMed 19228121).supplied by OMIM, Dec 2009
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Documents
Resources
"NM_080876" in other vectors (6)
SKU Description Size Price
RC206095L1 Lenti ORF clone of Human dual specificity phosphatase 19 (DUSP19), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC206095L2 Lenti ORF clone of Human dual specificity phosphatase 19 (DUSP19), transcript variant 1, mGFP tagged 10 ug
$600.00
RC206095L3 Lenti ORF clone of Human dual specificity phosphatase 19 (DUSP19), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC206095L4 Lenti ORF clone of Human dual specificity phosphatase 19 (DUSP19), transcript variant 1, mGFP tagged 10 ug
$600.00
RG206095 DUSP19 (tGFP-tagged) - Human dual specificity phosphatase 19 (DUSP19), transcript variant 1 10 ug
$489.00 $500.00
SC128060 DUSP19 (untagged)-Human dual specificity phosphatase 19 (DUSP19), transcript variant 1 10 ug
$300.00

Plasmid Map for RC206095

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206095
Circular map for RC206095

file-alt Citations

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