PRPS2 (NM_002765) Human Tagged ORF Clone

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SKU
RC206052
PRPS2 (Myc-DDK-tagged)-Human phosphoribosyl pyrophosphate synthetase 2 (PRPS2), transcript variant 2
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PRPS2
Synonyms PRSII
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206052 representing NM_002765
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAACATCGTGCTGTTCAGCGGCAGCTCGCATCAGGACCTGTCCCAGCGCGTGGCCGACCGCCTGG
GCCTGGAGCTGGGCAAGGTGGTCACGAAGAAGTTCAGCAACCAGGAGACCAGCGTGGAGATTGGTGAAAG
CGTGAGAGGGGAAGATGTCTACATCATCCAGAGCGGCTGCGGGGAAATTAACGACAACCTGATGGAACTC
CTCATCATGATCAATGCCTGCAAGATTGCGTCATCATCCAGAGTAACTGCCGTGATCCCGTGTTTCCCAT
ACGCCCGACAAGATAAAAAGGACAAGAGTCGTGCCCCAATTTCTGCAAAACTTGTGGCCAATATGCTGTC
GGTGGCTGGGGCGGATCACATCATCACCATGGACCTGCATGCTTCTCAGATACAGGGATTCTTTGATATT
CCTGTGGATAATTTGTATGCGGAGCCCGCAGTCCTGCAGTGGATTCGGGAAAACATTGCCGAGTGGAAGA
ACTGTATCATTGTTTCACCTGACGCAGGGGGAGCCAAAAGGGTTACATCAATTGCAGACAGGTTGAATGT
GGAATTTGCTTTGATCCACAAAGAGAGGAAGAAGGCGAATGAAGTGGACCGGATGGTCCTGGTGGGCGAC
GTGAAGGACCGTGTGGCCATCCTCGTGGATGACATGGCTGACACTTGCGGCACCATCTGCCATGCTGCGG
ACAAGCTGCTGTCAGCTGGAGCCACCAAAGTGTATGCTATCCTTACCCATGGGATCTTCTCTGGACCAGC
TATTTCCAGAATAAATAATGCCGCCTTTGAGGCTGTTGTCGTCACAAACACAATTCCGCAAGAGGACAAA
ATGAAACACTGCACCAAGATTCAGGTCATTGACATTTCCATGATCTTGGCCGAAGCAATCCGAAGGACAC
ACAATGGGGAATCCGTGTCCTACCTGTTCAGCCATGTCCCGCTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206052 representing NM_002765
Red=Cloning site Green=Tags(s)

MPNIVLFSGSSHQDLSQRVADRLGLELGKVVTKKFSNQETSVEIGESVRGEDVYIIQSGCGEINDNLMEL
LIMINACKIASSSRVTAVIPCFPYARQDKKDKSRAPISAKLVANMLSVAGADHIITMDLHASQIQGFFDI
PVDNLYAEPAVLQWIRENIAEWKNCIIVSPDAGGAKRVTSIADRLNVEFALIHKERKKANEVDRMVLVGD
VKDRVAILVDDMADTCGTICHAADKLLSAGATKVYAILTHGIFSGPAISRINNAAFEAVVVTNTIPQEDK
MKHCTKIQVIDISMILAEAIRRTHNGESVSYLFSHVPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002765
ORF Size 954 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002765.5
RefSeq Size 2509 bp
RefSeq ORF 957 bp
Locus ID 5634
UniProt ID P11908
Cytogenetics Xp22.2
Domains Pribosyltran
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Pentose phosphate pathway, Purine metabolism
MW 34.6 kDa
Summary This gene encodes a phosphoribosyl pyrophosphate synthetase that plays a central role in the synthesis of purines and pyrimidines. The encoded protein catalyzes the synthesis of 5-phosphoribosyl 1-pyrophosphate from ATP and D-ribose 5-phosphate. Alternate splicing results in multiple transcript variants. provided by RefSeq, Mar 2010
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Documents
Resources
"NM_002765" in other vectors (4)
SKU Description Size Price
RC206052L3 Lenti ORF clone of Human phosphoribosyl pyrophosphate synthetase 2 (PRPS2), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC206052L4 Lenti ORF clone of Human phosphoribosyl pyrophosphate synthetase 2 (PRPS2), transcript variant 2, mGFP tagged 10 ug
$600.00
RG206052 PRPS2 (tGFP-tagged) - Human phosphoribosyl pyrophosphate synthetase 2 (PRPS2), transcript variant 2 10 ug
$500.00
SC118398 PRPS2 (untagged)-Human phosphoribosyl pyrophosphate synthetase 2 (PRPS2), transcript variant 2 10 ug
$300.00

Plasmid Map for RC206052

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Cloning scheme for RC206052

Circular map for RC206052

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