FOXA1 (NM_004496) Human Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | FOXA1 |
| Synonyms | HNF3A; TCF3A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004496 |
| ORF Size | 1416 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004496.5 |
| RefSeq Size | 3396 bp |
| RefSeq ORF | 1419 bp |
| Locus ID | 3169 |
| UniProt ID | P55317 |
| Cytogenetics | 14q21.1 |
| Domains | FH |
| Protein Families | ES Cell Differentiation/IPS, Transcription Factors |
| MW | 49.1 kDa |
| Summary | This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004496" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206045L1 | Lenti ORF clone of Human forkhead box A1 (FOXA1), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC206045L2 | Lenti ORF clone of Human forkhead box A1 (FOXA1), mGFP tagged | 10 ug |
$986.00
|
|
| RC206045L3 | Lenti ORF clone of Human forkhead box A1 (FOXA1), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC206045L4 | Lenti ORF clone of Human forkhead box A1 (FOXA1), mGFP tagged | 10 ug |
$986.00
|
|
| RG206045 | FOXA1 (tGFP-tagged) - Human forkhead box A1 (FOXA1) | 10 ug |
$886.00
|
|
| SC108256 | FOXA1 (untagged)-Human forkhead box A1 (FOXA1) | 10 ug |
$457.00
|
|
Citations
Elevated FOXA1 Expression Indicates Poor Prognosis in Liver Cancer due to Its Effects on Cell Proliferation and Metastasis
Liu, Wang, Aizimuaji et al
Dis Markers (2022) 2022, 3317315 DOI: 10.1155/2022/3317315
Liu, Wang, Aizimuaji et al
Dis Markers (2022) 2022, 3317315 DOI: 10.1155/2022/3317315
Cited by 9
AI Summary: OriGene products, specifically the pCMV6-FOXA1 vector (Cat#: RC206045) and the pCMV6-entry vector, were used to manipulate FOXA1 expression in liver cancer cells, enabling the study of FOXA1's role in cell proliferation and metastasis in this research.
Snippet: … Both the pCMV6-FOXA1 vector (Art.: RC206045) and pCMV6-entry vector used in this study were purchased from the OriGene Company in the United States. A carbon dioxide incubator (Heraeus, Germany), an electrophoresis tank with a wet transfer electrometer (Bio-Rad, USA), a chemiluminescence imaging system (GE, U;- RC206045) and pCMV6-entry vector used in this study were purchased from the OriGene Company in the United States. …
FOXA1 Mutations Reveal Distinct Chromatin Profiles and Influence Therapeutic Response in Breast Cancer
Arruabarrena-Aristorena, Maag, Kittane et al
Cancer Cell (2020) 38 (4), 534-550.e9 DOI: 10.1016/j.ccell.2020.08.003
Arruabarrena-Aristorena, Maag, Kittane et al
Cancer Cell (2020) 38 (4), 534-550.e9 DOI: 10.1016/j.ccell.2020.08.003
Cited by 141
AI Summary: OriGene’s pCMV6-FOXA1 cDNA (NM_004496) was used as the source for generating wild-type and mutant FOXA1 constructs, enabling the study of how specific FOXA1 mutations affect chromatin profiles and therapeutic responses in breast cancer.
Snippet: To obtain FOXA1 mutant constructs FOXA1 cDNA from pCMV6-FOXA1 (Origene, NM_004496) was first subcloned into a pDONR223 vector using Gateway technology from Thermo Fisher. pDONR223_FOXA1_WT was then engineered by site directed mutagenesis to introduce recurrent mutations occurring in patients using the Q5 site-directed mutagenesis kit from New England Biolabs (NEB), according to the manufacturer’s protocol. … Finally, pDONR223 constructs with either FOXA1 WT or mutant cDNA were subcloned into the pLenti6_Ubc_V5 lentiviral vector. …
Hepatocyte Nuclear Factor 4α regulates expression of the serotonin transporter in intestinal epithelial cells
Holton, Singhal, Kumar et al
Am. J. Physiol., Cell Physiol. (2020)
Holton, Singhal, Kumar et al
Am. J. Physiol., Cell Physiol. (2020)
Cited by 7
AI Summary: In this study, the FoxA1 plasmid from OriGene was used to investigate the role of different transcription factors in regulating serotonin transporter expression in intestinal epithelial cells.
Snippet: Page 8. 8 HNF1β, HNF4α were purchased from Addgene (Watertown, MA) and FoxA1 from 207 Origene (Rockville, MD) and siRNA was purchased from Qiagen (Germantown, MD). 208 The truncated SERT promoter sequences (Δ1, Δ2, Δ3, Δ4) were constructed using
FOXA1, GATA3 and PPARɣ Cooperate to Drive Luminal Subtype in Bladder Cancer: A Molecular Analysis of Established Human Cell Lines
Warrick, Walter, Yamashita et al
Sci Rep (2016) 6, 38531 DOI: 10.1038/srep38531
Warrick, Walter, Yamashita et al
Sci Rep (2016) 6, 38531 DOI: 10.1038/srep38531
AI Summary: OriGene products pCMV6-Entry (empty vector), pCMV6-FOXA1 (RC206045), and pCMV6-GATA3 (RC211904) were used to transfect bladder cancer cell lines, enabling the study of how FOXA1 and GATA3 overexpression contributes to luminal subtype differentiation.
Snippet: The following day, attached cells were transfected with the following plasmid constructs: pCMV6-Entry (CMV empty vector;Origene, Rockville MD), pCMV6-FOXA1 (Origene;RC206045), pCMV6-GATA3 (Origene;RC211904), or a combination of pCMV6-FOXA1 and pCMV6-GATA3 using Lipofectamine 3000 (Life Technologies, Carlsbad CA),
FOXA1 modulates EAF2 regulation of AR transcriptional activity, cell proliferation, and migration in prostate cancer cells
Guo, Keener, Jing et al
Prostate (2015)
Guo, Keener, Jing et al
Prostate (2015)
Cited by 37
Tumor suppressor long non-coding RNA, MT1DP is negatively regulated by YAP and Runx2 to inhibit FoxA1 in liver cancer cells
Yu, Qiao, Tang et al
Cell. Signal. (2014) 26 (12), 2961-2968 DOI: 10.1016/j.cellsig.2014.09.011
Yu, Qiao, Tang et al
Cell. Signal. (2014) 26 (12), 2961-2968 DOI: 10.1016/j.cellsig.2014.09.011
Cited by 106
Showing 1-6 of 7 papers.
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