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FOXA1 (NM_004496) Human Untagged Clone

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SKU
SC108256
FOXA1 (untagged)-Human forkhead box A1 (FOXA1)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol FOXA1
Synonyms HNF3A; TCF3A
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
NCBI ORF sequence for NM_004496, the custom clone sequence may differ by one or more nucleotides
GAATTCGGCACGAGGGTGGCGTTGGGCCCGCGCGGGCGCTCGGGTGACTGCAGCTGCTCA
GCTCCCCTCCCCCGCCCCGCGCCGCGCGGCCGCCCGTCGCTTCGCACAGGGCTGGATGGT
TGTATTGGGCAGGGTGGCTCCAGGATGTTAGGAACTGTGAAGATGGAAGGGCATGAAACC
AGCGACTGGAACAGCTACTACGCAGACACGCAGGAGGCCTACTCCTCCGTCCCGGTCAGC
AACATGAACTCAGGCCTGGGCTCCATGAACTCCATGAACACCTACATGACCATGAACACC
ATGACTACGAGCGGCAACATGACCCCGGCGTCCTTCAACATGTCCTATGCCAACCCGGGC
CTAGGGGCCGGCCTGAGTCCCGGCGCAGTAACCGGCATGCCGGGGGGCTCGGCGGGCGCC
ATGAACAGCATGACTGCGGCCGGCGTGACGGCCATGGGTACGGCGCTGAGCCCGAGCGGC
ATGGGCGCCATGGGTGCGCAGCAGGCGGCCTCCATGAATGGCCTGGGCCCCTACGCGGCC
GCCATGAACCCGTGCATGAGCCCCATGGCGTACGCGCCGTCCAACCTGGGCCGCAGCCGC
GCGGGCGGCGGCGGCGACGCCAAGACGTTCAAGCGCAGCTACCCGCACGCCAAGCCGCCC
TACTCGTACATCTCGCTCATCACCATGGCCATCCAGCAGGCGCCCAGCAAGATGCTCACG
CTGAGCGAGATCTACCAGTGGATCATGGACCTCTTCCCCTATTACCGGCAGAACCAGCAG
CGCTGGCAGAACTCCATCCGCCACTCGCTGTCCTTCAATGACTGCTTCGTCAAGGTGGCA
CGCTCCCCGGACAAGCCGGGCAAGGGCTCCTACTGGACGCTGCACCCGGACTCCGGCAAC
ATGTTCGAGAACGGCTGCTACTTGCGCCGCCAGAAGCGCTTCAAGTGCGAGAAGCAGCCG
GGGGCCGGCGGCGGGGGCGGGAGCGGAAGCGGGGGCAGCGGCGCCAAGGGCGGCCCTGAG
AGCCGCAAGGACCCCTCTGGCGCCTCTAACCCCAGCGCCGACTCGCCCCTCCATCGGGGT
GTGCACGGGAAGACCGGCCAGCTAGAGGGCGCGCCGGCCCCCGGGCCCGCCGCCAGCCCC
CAGACTCTGGACCACAGTGGGGCGACGGCGACAGGGGGCGCCTCGGAGTTGAAGACTCCA
GCCTCCTCAACTGCGCCCCCCATAAGCTCCGGGCCCGGGGCGCTGGCCTCTGTGCCCGCC
TCTCACCCGGCACACGGCTTGGCACCCCACGAGTCCCAGCTGCACCTGAAAGGGGACCCC
CACTACTCCTTCAACCACCCGTTCTCCATCAACAACCTCATGTCCTCCTCGGAGCAGCAG
CATAAGCTGGACTTCAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACG
TTGCCCGCCAGCCTGCCTCTAGGCAGCGCCTCGGTGACCACCAGGAGCCCCATCGAGCCC
TCAGCCCTGGAGCCGGCGTACTACCAAGGTGTGTATTCCAGACCCGTCCTAAACACTTCC
TAGCTCCCGGGACTGGGGGGTTTGTCTGGCATAGCCATGCTGGTAGCAAGAGAGAAAAAA
TCAACAGCAAACAAAACCACACAAACCAAACCGTCAACAGCATAATAAAATCCCAACAAC
TATTTTTATTTCATTTTTCATGCACAACCTTTCCCCCAGTGCAAAAGACTGTTACTTTAT
TATTGTATTCAAAATTCATTGTGTATATTACTACAAAGACAACCCCAAACCAATTTTTTT
CCTGCGAAGTTTAATGATCCACAAGTGTATATATGAAATTCTCCTCCTTCCTTGCCCCCC
TCTCTTTCTTCCCTCTTTCCCCTCCAGACATTCTAGTTTGTGGAGGGTTATTTAAAAAAA
CAAAAAAGGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004496 unedited
GTGCAGAATATTTGTATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGGTGGC
GTTGGGCCCGCGCGGGCGCTCGGGTGACTGCAGCTGCTCAGCTCCCCTCCCCCGCCCCGC
GCCGCGCGGCCGCCCGTCGCTTCGCACAGGGCTGGATGGTTGTATTGGGCAGGGTGGCTC
CAGGATGTTAGGAACTGTGAAGATGGAAGGGCATGAAACCAGCGACTGGAACAGCTACTA
CGCAGACACGCAGGAGGCCTACTCCTCCGTCCCGGTCAGCAACATGAACTCAGGCCTGGG
CTCCATGAACTCCATGAACACCTACATGACCATGAACACCATGACTACGAGCGGCAACAT
GACCCCGGCGTCCTTCAACATGTCCTATGCCAACCCGGGCCTAGGGGCCGGCCTGAGTCC
CGGCGCAGTAACCGGCATGCCGGGGGGCTCGGCGGGCGCCATGAACAGCATGACTGCGGC
CGGCGTGACGGCCATGGGTACGGCGCTGAGCCCGAGCGGCATGGGCGCCATGGGTGCGCA
GCAGGCGGCCTCCATGAATGGCCTGGGCCCCTACGCGGCCGCCATGAACCCGTGCATGAG
CCCCATGGCGTACGCGCCGTCCAAACCTGTGCCGCACCGCGCGGGCGGCGGCGGCGACGC
CAAGACGTTCAAGCGCAGCTACCCGCACGCCAAGCCCGCCCTACTCGTACATCTTGCTCA
TCACCATGGCCATCCAGCAGGCGCCCAGCAAGATGCTCACGCTGAGCCAGATCTTACAGT
GGATCATGGACCTCTTTCCCTATTACCCGCCGAACCAGCATCGCTGGCAGAACTCCATCC
CCACTCGCTGTCCTTTAATGACTGCGTCGTCCAGGTGGCACCCTCCCCCGACAAGCCGGG
CCAAGGCTCCTACTGGACCCTTGACCCGGCTCCG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004496 unedited
GGCCGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTAACATTTGATTTATTTAAA
GTCCTTAACTGCAAATGATCAAGAACATTTTCCACGGCTTAAAATCTGGTATTATTTTTG
TTATTTAAATCAAAATATTTTTAATTACAATCACATTGATAAAAATTAACAATTTAATTT
GTAATTTTAACCATCATTTAAAGCAATTTCAGCTGTAAAGAAAAAGAATAAACCATGCAA
TAAATTAACATTGAGAAAGCAAAGAGAAATAATAATGAAACCCGTCTGGCTATACTAACA
CCATGTCCAACTGTGGAAAGTGCATAACACTGGATGAAACAAGCACACGATGGCAATGAT
TAAAATGGCCACTATCAATAGGATTTTTGAATCTTGGACCACGTTTTGAAATCCAGCTCC
CTATAACTTATCTCTCCTCCAACATTGTAATATTCCCTTTTACTGAAAAAAAAATTCTTT
AAATGGTATACACTCATAGAAACTAATTTTATGTGTTATGTGACCTCAGAATGACATGAC
CATGGCACTCTGCAAAGCAAGAAGCAGAGTTCTTGAGGGCAATTCCTGAGGAATTGATTC
CCACGGCCATCTGTGGGTAGAGAGGACAAAGGGGTTGGGGGCCTTGTAACCTTCGCCAAG
CAACTCTTGAGAATGATCATGGATTCACCTATTTTTACCTCTAATGGTATGTAAAATACG
GAGGATGGCTCCCATTTGACCATGTGCCTATTAAGCCCCTAACATATCCCTGTTTTCCAT
TTAAGACCCGTGGTCTCCCCATGCCTCGAAAGGAAACTGGGAACAATGGCTCCCCAGTTC
TAAAGGNGAAGGGCGGGCATATACACCACATGGGCTCACTACCTTCACAGCCCTAAGTGA
ACACGCCCTCAGCCTCCAATCCCTTGGTAAACCCTGCGTCGCGCGTACCGCGGGGGCG
Restriction Sites NotI-NotI |
ACCN NM_004496
Insert Size 1419 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004496.2, NP_004487.2
RefSeq Size 3124 bp
RefSeq ORF 1419 bp
Locus ID 3169
UniProt ID P55317
Cytogenetics 14q21.1
Domains FH
Protein Families ES Cell Differentiation/IPS, Transcription Factors
Summary This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_004496" in other vectors (6)
SKU Description Size Price
RC206045 FOXA1 (Myc-DDK-tagged)-Human forkhead box A1 (FOXA1) 10 ug
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RC206045L1 Lenti ORF clone of Human forkhead box A1 (FOXA1), Myc-DDK-tagged 10 ug
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RC206045L2 Lenti ORF clone of Human forkhead box A1 (FOXA1), mGFP tagged 10 ug
$986.00
RC206045L3 Lenti ORF clone of Human forkhead box A1 (FOXA1), Myc-DDK-tagged 10 ug
$986.00
RC206045L4 Lenti ORF clone of Human forkhead box A1 (FOXA1), mGFP tagged 10 ug
$986.00
RG206045 FOXA1 (tGFP-tagged) - Human forkhead box A1 (FOXA1) 10 ug
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file-alt Citations

Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer
Parolia, Cieslik, Chu et al
Nature (2019) 571 (7765), 413-418 DOI: 10.1038/s41586-019-1347-4
Cited by 303
AI Summary: OriGene’s WT FOXA1 coding sequence (Cat#: SC108256) was used as the template to generate wild-type and mutant FOXA1 constructs for functional studies in this paper.
Snippet: WT FOXA1 coding sequence was purchased from Origene (Cat#: SC108256) and cloned into the pLenti6/V5 lentiviral vector (ThermoFisher Scientific;Cat#: K4955–10) using the standard TOPO cloning protocol. Class1 missense mutations (I176M;H247Q and R261G) were engineered from the WT FOXA1 vector using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Tech) as per manufacturer’s instructions. …All FOXA1 variants had the V5-tag fused on the C-terminus. …
FigureFigureFigure
MicroRNA‑212 inhibits the proliferation and invasion of human renal cell carcinoma by targeting FOXA1
Tong, Meng, Wang et al
Mol Med Rep (2018) 17 (1), 1361-1367 DOI: 10.3892/mmr.2017.7956
Cited by 10
AI Summary: OriGene products contributed to this study by providing the human FOXA1 overexpression plasmid (cat. no. SC108256), which was used to assess the functional role of FOXA1 in renal cell carcinoma cells.
Snippet: mimic were purchased from GeneCopoeia, Inc. (Rockville, MD, USA). Plasmids carrying human FOXA1 were purchased from OriGene Technologies, Inc. (Rockville, MD, USA;cat. no. SC108256). Cells were seeded in a six
Identification and characterization of a FOXA2-regulated transcriptional enhancer at a type 2 diabetes intronic locus that controls GCKR expression in liver cells
López Rodríguez, Kaminska, Lappalainen et al
Genome Med (2017) 9 (1), 63 DOI: 10.1186/s13073-017-0453-x
AI Summary: OriGene products, specifically the plasmids expressing human FOXA2 (SC122913), FOXA1 (SC118299), RXRA (SC118696), MAFK (SC108256), and the control vector pCMV6-XL5, were used to assess the role of these transcription factors in regulating the GCKR intronic enhancer activity in liver cells.
Snippet: plasmid (Promega) encoding the luciferase gene hRluc (Renilla reniformis) and plasmids expressing human FOXA2, FOXA1, RXRA, or MAFK or the control vector pCMV6-XL5 without TF insert (SC122913;SC118299;SC118696;SC108256;pCMV6-XL5;OriGene) ... All 6 versions Cite SaveSaving
FigureFigureFigure
MicroRNA-212 suppresses tumor growth of human hepatocellular carcinoma by targeting FOXA1
Dou, Wang, Li et al
Oncotarget (2015) 6 (15), 13216-28 DOI: 10.18632/oncotarget.3916
Cited by 77
AI Summary: OriGene products, specifically the FOXA1 cDNA clone and FOXA1 shRNA lentiviral particles, were used in this study to manipulate FOXA1 expression and investigate its role as a direct target of miR-212 in hepatocellular carcinoma cells.
FigureFigureFigure
Direct Reprogramming of Human Fibroblasts to Hepatocyte-Like Cells by Synthetic Modified mRNAs
Simeonov, Uppal
PLoS ONE (2014) 9 (6), e100134 DOI: 10.1371/journal.pone.0100134
Cited by 92
AI Summary: OriGene contributed plasmids containing human open reading frames (ORFs) for C/EBPA, FOXA1, FOXA2, FOXA3, GATA4, GATA6, HHEX, HNF1A, HNF1B, HNF4A, and HNF6A, which were used as templates for generating the synthetic mRNAs essential for direct reprogramming of human fibroblasts to hepatocyte-like cells in this study.
Snippet: Open reading frame (ORF) PCR amplifications of DNA encoding C/EBPA FOXA1, FOXA2, FOXA3, GATA4, GATA6, HHEX, HNF1A, HNF1B, HNF4A, and HNF6Awere templated from DNA plasmids containing each of the respective human ORFs (Origene, Rockville, ... Cite SaveSaving
FigureFigureFigure
Showing 1-5 of 5 papers.
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