MSH2 (NM_000251) Human Tagged ORF Clone
SKU
RC205848
MSH2 (Myc-DDK-tagged)-Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2)
$855.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MSH2 |
| Synonyms | COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2; MMRCS2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000251 |
| ORF Size | 2802 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000251.3 |
| RefSeq Size | 3226 bp |
| RefSeq ORF | 2805 bp |
| Locus ID | 4436 |
| UniProt ID | P43246 |
| Cytogenetics | 2p21-p16.3 |
| Domains | MutS_I, MutS_II, MutS_III, MutS_IV, MutS_V |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Colorectal cancer, Mismatch repair, Pathways in cancer |
| MW | 104.7 kDa |
| Summary | This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Apr 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000251" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205848L1 | Lenti ORF clone of Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2), Myc-DDK-tagged | 10 ug |
$1,155.00
|
|
| RC205848L2 | Lenti ORF clone of Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2), mGFP tagged | 10 ug |
$1,155.00
|
|
| RC205848L3 | Lenti ORF clone of Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2), Myc-DDK-tagged | 10 ug |
$1,155.00
|
|
| RC205848L4 | Lenti ORF clone of Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2), mGFP tagged | 10 ug |
$1,155.00
|
|
| RG205848 | MSH2 (tGFP-tagged) - Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2) | 10 ug |
$789.00
$1,055.00
|
|
| SC119995 | MSH2 (untagged)-Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2) | 10 ug |
$1,284.00
|
|
Citations
Replication fork stalling in late S-phase elicits nascent strand degradation by DNA mismatch repair
Colicino-Murbach, Hathaway, Dungrawala
Nucleic Acids Res (2024)
Colicino-Murbach, Hathaway, Dungrawala
Nucleic Acids Res (2024)
Cited by 8
AI Summary: OriGene’s CMV-MSH2 Myc-DDK-tagged (RC205848) and CMV-MLH1 Myc-DDK-tagged (RC201607) plasmids were used for overexpression studies to investigate the roles of DNA mismatch repair proteins in nascent strand degradation at stalled replication forks.
Snippet: mid transfections, RPE-1 cells were transfected using Lipofectamine 2000 (Invitrogen 11668027) in Opti-MEM Reduced Serum Media for 48–72 h using the following constructs: CMV-hRAD51 (Addgene Plasmid #125570), CMVhRAD51K133R (Addgene Plasmid #125571), CMV-MSH2 Myc-DDK-tagged (Origene RC205848) and CMV-MLH1 Myc-DDK-tagged (Origene RC201607)
Dianhydrogalactitol synergizes with topoisomerase poisons to overcome DNA repair activity in tumor cells
Zhai, Li, Kotapalli et al
Cell Death Dis (2020) 11 (7), 577 DOI: 10.1038/s41419-020-02780-8
Zhai, Li, Kotapalli et al
Cell Death Dis (2020) 11 (7), 577 DOI: 10.1038/s41419-020-02780-8
Cited by 8
AI Summary: OriGene products contributed to this study by providing human MLH1 cDNA (vector RC201607), MSH2 cDNA (vector RC205848), and the pLenti-C-Myc-DDK-P2A-Puro vector (PS100092), which were used to generate stable cell lines for investigating the role of DNA repair pathways in tumor cell response to dianhydrogalactitol and chemotherapy.
Snippet: B3, siBRCA1–25 (SI04381377, Qiagen);B4, siBRCA1–15 (SI02664368, Qiagen);and B5, siBRCA1–17 (SI03103975, Qiagen). STABLE CELL LINE GENERATION Human MLH1 cDNA (obtained from vector RC201607, OriGene Technologies, Rockville, MD, USA) or MSH2 cDNA (obtained from vector RC205848, OriGene Technologies) was subcloned into pLenti-C-Myc-DDK-P2A-Puro vector (PS100092, OriGene Technologies) using;- 1,2:5,6-Dianhydrogalactitol (DAG) is a bi-functional DNA-targeting agent currently in phase II clinical trial for treatment of temozolomide-resistant glioblastoma (GBM). In the present study, we investigated the cytotoxic activity of DAG alone or in combination with common;and B5, siBRCA1–17 (SI03103975, Qiagen). Stable cell line generation Human MLH1 cDNA (obtained from vector RC201607, OriGene Technologies, Rockville, MD, USA) or MSH2 cDNA (obtained from vector RC205848, OriGene Technologies) was subcloned into pLenti-C-Myc-DDK-P2A-Puro vector (PS100092, OriGene Technologies) using
Retinal mitochondrial DNA mismatch repair in the development of diabetic retinopathy, and its continued progression after termination of hyperglycemia
Mishra, Kowluru
Invest. Ophthalmol. Vis. Sci. (2014)
Mishra, Kowluru
Invest. Ophthalmol. Vis. Sci. (2014)
Cited by 74
Showing 1-3 of 3 papers.
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