PGAP6 (NM_021259) Human Tagged ORF Clone

SKU RC205822

TMEM8A (Myc-DDK-tagged)-Human transmembrane protein 8A (TMEM8A)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PGAP6
• Synonyms: GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205822 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGCCGGGCTGGCACCGGGACCGGGGGCGAGGCGGTGGCCGCGGTGGTGGCGGGGCCGCTGCTGCTGC; TGCTGCTTGCCCGGCCCCCGCCTGCCTCCGCCGGCTACAGCGGGAAGAGCGAGGTGGGGCTGGTGTCCGA; GCACTTCTCACAGGCCCCGCAGAGGCTGTCCTTCTACAGCTGGTATGGCAGTGCCAGGCTCTTCCGCTTC; CGCGTGCCCCCAGATGCTGTGCTTCTACGCTGGCTCCTGCAGGTCTCCCGGGAGAGCGGCGCTGCCTGCA; CCGACGCGGAGATCACCGTGCACTTCCGTTCCGGCGCCCCTCCGGTCATCAACCCGCTGGGCACCAGCTT; CCCGGACGACACCGCGGTGCAGCCCTCCTTCCAGGTCGGGGTGCCGCTGAGCACCGCACCGAGAAGCAAT; GCCTCCGTCAACGTTTCCCACCCGGCCCCCGGGGACTGGTTCGTGGCCGCCCACCTGCCCCCCTCATCCC; AGAAGATCGAGTTGAAGGGCTTGGCTCCCACCTGTGCCTACGTCTTCCAGCCTGAACTGCTGGTCACGCG; GGTGGTCGAGATTTCCATCATGGAGCCGGACGTGCCCCTTCCTCAGACCCTCCTCTCCCATCCCAGCTAC; CTCAAGGTCTTTGTCCCCGATTACACGCGGGAGCTTCTGCTGGAGCTGCGGGACTGCGTGTCCAATGGGA; GCCTGGGCTGCCCCGTGCGTCTCACCGTGGGCCCGGTCACCCTGCCTAGCAACTTCCAGAAGGTGCTCAC; CTGCACCGGTGCCCCCTGGCCCTGCCGCCTGCTGCTGCCCTCACCGCCCTGGGACCGGTGGCTGCAAGTG; ACAGCTGAGAGCCTGGTGGGGCCCCTCGGGACAGTGGCTTTCAGTGCTGTAGCTGCCCTCACAGCTTGCA; GGCCACGGAGCGTGACCGTCCAGCCCCTTCTGCAGAGCAGCCAAAACCAGAGCTTCAATGCCTCCTCTGG; TCTGCTGTCCCCGAGCCCCGACCACCAGGACCTGGGCAGGAGTGGCAGGGTGGACCGCAGCCCCTTCTGC; CTCACAAACTACCCAGTCACGCGGGAGGACATGGACGTGGTGTCGGTGCACTTCCAGCCCCTGGACAGGG; TCTCGGTGAGGGTGTGTTCGGACACGCCCTCCGTGATGCGGCTGCGCCTGAACACCGGCATGGACAGCGG; GGGTTCCCTCACCATCTCCCTGCGGGCCAACAAGACAGAGATGCGGAACGAGACCGTCGTAGTGGCCTGC; GTGAATGCTGCCTCGCCCTTCCTTGGCTTCAATACTTCGCTCAACTGCACCACAGCCTTCTTCCAGGGCT; ACCCTTTGTCTCTGAGCGCCTGGTCTCGCAGGGCCAACCTCATCATCCCCTACCCAGAGACAGACAACTG; GTACCTCTCCCTGCAGCTCATGTGCCCTGAGAATGCTGAGGACTGTGAGCAGGCTGTGGTCCACGTGGAG; ACCACCTTGTACCTGGTGCCCTGTTTGAACGATTGTGGACCCTATGGCCAGTGCCTCCTGCTCCGCAGAC; ACAGCTACCTGTATGCCAGCTGCAGCTGCAAGGCAGGCTGGCGTGGGTGGAGCTGCACGGACAACAGCAC; AGCCCAGACGGTGGCCCAGCAGAGGGCGGCCACACTGCTGCTCACGCTCAGCAACCTCATGTTCCTGGCC; CCCATCGCCGTCTCAGTGCGGCGATTCTTCCTGGTGGAGGCCTCCGTCTACGCCTACACCATGTTCTTCT; CCACGTTCTACCACGCCTGCGACCAGCCCGGGGAGGCGGTGCTGTGCATCCTCAGCTACGACACGCTGCA; GTACTGCGACTTCTTGGGCTCCGGGGCGGCCATCTGGGTCACCATCCTGTGCATGGCACGGCTCAAGACA; GTCCTGAAATACGTGCTGTTTCTTCTGGGTACACTGGTCATCGCCATGTCCTTGCAGCTGGACCGCAGGG; GCATGTGGAACATGCTGGGGCCCTGCCTCTTTGCCTTCGTGATCATGGCCTCCATGTGGGCTTACCGCTG; CGGGCACCGGCGCCAGTGCTACCCCACCTCGTGGCAGCGCTGGGCCTTCTACCTCCTGCCCGGCGTCTCT; ATGGCCTCTGTGGGCATCGCCATCTACACCTCCATGATGACTAGCGACAACTACTACTACACCCACAGCA; TCTGGCACATCCTGCTGGCCGGGAGCGCAGCCTTGCTGCTGCCGCCACCTGACCAGCCCGCCGAGCCCTG; GGCCTGCTCGCAGAAATTCCCCTGCCACTATCAGATCTGCAAGAACGATCGGGAGGAACTGTACGCAGTG; ACG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205822 protein sequence; Red=Cloning site Green=Tags(s); ; MGRAGTGTGGEAVAAVVAGPLLLLLLARPPPASAGYSGKSEVGLVSEHFSQAPQRLSFYSWYGSARLFRF; RVPPDAVLLRWLLQVSRESGAACTDAEITVHFRSGAPPVINPLGTSFPDDTAVQPSFQVGVPLSTAPRSN; ASVNVSHPAPGDWFVAAHLPPSSQKIELKGLAPTCAYVFQPELLVTRVVEISIMEPDVPLPQTLLSHPSY; LKVFVPDYTRELLLELRDCVSNGSLGCPVRLTVGPVTLPSNFQKVLTCTGAPWPCRLLLPSPPWDRWLQV; TAESLVGPLGTVAFSAVAALTACRPRSVTVQPLLQSSQNQSFNASSGLLSPSPDHQDLGRSGRVDRSPFC; LTNYPVTREDMDVVSVHFQPLDRVSVRVCSDTPSVMRLRLNTGMDSGGSLTISLRANKTEMRNETVVVAC; VNAASPFLGFNTSLNCTTAFFQGYPLSLSAWSRRANLIIPYPETDNWYLSLQLMCPENAEDCEQAVVHVE; TTLYLVPCLNDCGPYGQCLLLRRHSYLYASCSCKAGWRGWSCTDNSTAQTVAQQRAATLLLTLSNLMFLA; PIAVSVRRFFLVEASVYAYTMFFSTFYHACDQPGEAVLCILSYDTLQYCDFLGSGAAIWVTILCMARLKT; VLKYVLFLLGTLVIAMSLQLDRRGMWNMLGPCLFAFVIMASMWAYRCGHRRQCYPTSWQRWAFYLLPGVS; MASVGIAIYTSMMTSDNYYYTHSIWHILLAGSAALLLPPPDQPAEPWACSQKFPCHYQICKNDREELYAV; T; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021259
• ORF Size: 2313 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021259.3
• RefSeq Size: 3656 bp
• RefSeq ORF: 2316 bp
• Locus ID: 58986
• UniProt ID: Q9HCN3
• Cytogenetics: 16p13.3
• Protein Families: Transmembrane
• MW: 84.7 kDa
• Summary: Involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchor proteins (GPI-AP). Has phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI. Required for the shedding of the GPI-AP TDGF1, but not CFC1, at the cell surface. Shedding of TDGF1 modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells (PubMed:27881714). Also indirectly involved in the translocation of RAC1 from the cytosol to the plasma membrane by maintaining the steady state amount of CAV1-enriched plasma membrane subdomains, stabilizing RAC1 at the plasma membrane (PubMed:27835684). In contrast to myomaker (TMEM8C), has no fusogenic activity (PubMed:26858401).[UniProtKB/Swiss-Prot Function]