PGAP6 (NM_021259) Human Untagged Clone

SKU SC319584

TMEM8A (untagged)-Human transmembrane protein 8A (TMEM8A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PGAP6
• Synonyms: GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021259.1 GCCGGGCCGAGCGCGGAGCCATGGGCCGGGCTGGCACCGGGACCGGGGGCGAGGCGGTGG; CCGCGGTGGTGGCGGGGCCGCTGCTGCTGCTGCTGCTTGCCCGGCCCCCGCCTGCCTCCG; CCGGCTACAGCGGGAAGAGCGAGGTGGGGCTGGTGTCCGAGCACTTCTCACAGGCCCCGC; AGAGGCTGTCCTTCTACAGCTGGTATGGCAGTGCCAGGCTCTTCCGCTTCCGCGTGCCCC; CAGATGCTGTGCTTCTACGCTGGCTCCTGCAGGTCTCCCGGGAGAGCGGCGCTGCCTGCA; CCGACGCGGAGATCACCGTGCACTTCCGTTCCGGCGCCCCTCCGGTCATCAACCCGCTGG; GCACCAGCTTCCCGGACGACACCGCGGTGCAGCCCTCCTTCCAGGTCGGGGTGCCGCTGA; GCACCGCACCGAGAAGCAATGCCTCCGTCAACGTTTCCCACCCGGCCCCCGGGGACTGGT; TCGTGGCCGCCCACCTGCCCCCCTCATCCCAGAAGATCGAGTTGAAGGGCTTGGCTCCCA; CCTGTGCCTACGTCTTCCAGCCTGAACTGCTGGTCACGCGGGTGGTCGAGATTTCCATCA; TGGAGCCGGACGTGCCCCTTCCTCAGACCCTCCTCTCCCATCCCAGCTACCTCAAGGTCT; TTGTCCCCGATTACACGCGGGAGCTTCTGCTGGAGCTGCGGGACTGCGTGTCCAATGGGA; GCCTGGGCTGCCCCGTGCGTCTCACCGTGGGCCCGGTCACCCTGCCTAGCAACTTCCAGA; AGGTGCTCACCTGCACCGGTGCCCCCTGGCCCTGCCGCCTGCTGCTGCCCTCACCGCCCT; GGGACCGGTGGCTGCAAGTGACAGCTGAGAGCCTGGTGGGGCCCCTCGGGACAGTGGCTT; TCAGTGCTGTAGCTGCCCTCACAGCTTGCAGGCCACGGAGCGTGACCGTCCAGCCCCTTC; TGCAGAGCAGCCAAAACCAGAGCTTCAATGCCTCCTCTGGTCTGCTGTCCCCGAGCCCCG; ACCACCAGGACCTGGGCAGGAGTGGCAGGGTGGACCGCAGCCCCTTCTGCCTCACAAACT; ACCCAGTCACGCGGGAGGACATGGACGTGGTGTCGGTGCACTTCCAGCCCCTGGACAGGG; TCTCGGTGAGGGTGTGTTCGGACACGCCCTCCGTGATGCGGCTGCGCCTGAACACCGGCA; TGGACAGCGGGGGTTCCCTCACCATCTCCCTGCGGGCCAACAAGACAGAGATGCGGAACG; AGACCGTCGTAGTGGCCTGCGTGAATGCTGCCTCGCCCTTCCTTGGCTTCAATACTTCGC; TCAACTGCACCACAGCCTTCTTCCAGGGCTACCCTTTGTCTCTGAGCGCCTGGTCTCGCA; GGGCCAACCTCATCATCCCCTACCCAGAGACAGACAACTGGTACCTCTCCCTGCAGCTCA; TGTGCCCTGAGAATGCTGAGGACTGTGAGCAGGCTGTGGTCCACGTGGAGACCACCTTGT; ACCTGGTGCCCTGTTTGAACGATTGTGGACCCTATGGCCAGTGCCTCCTGCTCCGCAGAC; ACAGCTACCTGTATGCCAGCTGCAGCTGCAAGGCAGGCTGGCGTGGGTGGAGCTGCACGG; ACAACAGCACAGCCCAGACGGTGGCCCAGCAGAGGGCGGCCACACTGCTGCTCACGCTCA; GCAACCTCATGTTCCTGGCCCCCATCGCCGTCTCAGTGCGGCGATTCTTCCTGGTGGAGG; CCTCCGTCTACGCCTACACCATGTTCTTCTCCACGTTCTACCACGCCTGCGACCAGCCCG; GGGAGGCGGTGCTGTGCATCCTCAGCTACGACACGCTGCAGTACTGCGACTTCTTGGGCT; CCGGGGCGGCCATCTGGGTCACCATCCTGTGCATGGCACGGCTCAAGACAGTCCTGAAAT; ACGTGCTGTTTCTTCTGGGTACACTGGTCATCGCCATGTCCTTGCAGCTGGACCGCAGGG; GCATGTGGAACATGCTGGGGCCCTGCCTCTTTGCCTTCGTGATCATGGCCTCCATGTGGG; CTTACCGCTGCGGGCACCGGCGCCAGTGCTACCCCACCTCGTGGCAGCGCTGGGCCTTCT; ACCTCCTGCCCGGCGTCTCTATGGCCTCTGTGGGCATCGCCATCTACACCTCCATGATGA; CTAGCGACAACTACTACTACACCCACAGCATCTGGCACATCCTGCTGGCCGGGAGCGCAG; CCTTGCTGCTGCCGCCACCTGACCAGCCCGCCGAGCCCTGGGCCTGCTCGCAGAAATTCC; CCTGCCACTATCAGATCTGCAAGAACGATCGGGAGGAACTGTACGCAGTGACGTGACACT; GGCCTGGGGACAGCTGCTGCTCTGATGACCTCTTCAGCCAGGAGCTGTATCGAGGGGGAG; GCGCCTGGTCCAGCCCTGGACAGATTGATTTCCAGCTGAATAAATTGGCCTAGATACCAA; AAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_021259
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021259.1, NP_067082.1
• RefSeq Size: 2561 bp
• RefSeq ORF: 2316 bp
• Locus ID: 58986
• UniProt ID: Q9HCN3
• Cytogenetics: 16p13.3
• Protein Families: Transmembrane
• Summary: Involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchor proteins (GPI-AP). Has phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI. Required for the shedding of the GPI-AP TDGF1, but not CFC1, at the cell surface. Shedding of TDGF1 modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells (PubMed:27881714). Also indirectly involved in the translocation of RAC1 from the cytosol to the plasma membrane by maintaining the steady state amount of CAV1-enriched plasma membrane subdomains, stabilizing RAC1 at the plasma membrane (PubMed:27835684). In contrast to myomaker (TMEM8C), has no fusogenic activity (PubMed:26858401).[UniProtKB/Swiss-Prot Function]