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CHST8 (NM_022467) Human Tagged ORF Clone

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SKU
RC205818
CHST8 (Myc-DDK-tagged)-Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 (CHST8), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CHST8
Synonyms GalNAc4ST; GALNAC4ST1; PSS3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205818 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCTGCGACCTGGAACAATGCGGCTGGCCTGCATGTTCTCTTCCATCCTGCTGTTCGGAGCTGCAG
GCCTCCTCCTCTTCATCAGCCTGCAGGACCCTACGGAGCTCGCCCCCCAGCAGGTGCCAGGAATAAAGTT
CAACATCAGGCCAAGGCAGCCCCACCACGACCTCCCACCAGGCGGCTCCCAGGATGGTGACTTGAAGGAA
CCCACAGAGAGGGTCACTCGGGACTTATCCAGTGGGGCCCCGAGGGGCCGCAACCTGCCAGCGCCTGACC
AGCCTCAACCCCCGCTGCAGAGGGGAACCCGTCTGCGGCTCCGCCAGCGCCGTCGCCGTCTGCTCATCAA
GAAAATGCCAGCTGCGGCGACCATCCCGGCCAACAGCTCGGACGCGCCCTTCATCCGGCCGGGACCCGGG
ACGCTGGATGGCCGCTGGGTCAGCCTGCACCGGAGCCAGCAGGAGCGCAAGCGGGTGATGCAGGAGGCCT
GCGCCAAGTACCGGGCGAGCAGCAGCCGCCGGGCCGTCACGCCCCGCCACGTGTCCCGTATCTTCGTGGA
GGACCGCCACCGCGTGCTCTACTGCGAGGTGCCCAAGGCCGGCTGCTCCAATTGGAAGCGGGTGCTCATG
GTGCTGGCCGGCCTGGCCTCGTCCACTGCCGACATCCAGCACAACACCGTCCACTATGGCAGCGCTCTCA
AGCGCCTGGACACCTTCGACCGCCAGGGTATCTTGCACCGTCTCAGCACCTACACCAAGATGCTCTTTGT
CCGCGAGCCCTTCGAGAGGCTGGTGTCCGCCTTCCGCGACAAGTTTGAGCACCCCAACAGCTACTATCAC
CCGGTCTTCGGCAAGGCCATCCTGGCCCGGTACCGCGCCAATGCCTCTCGGGAGGCCCTGCGGACCGGCT
CTGGGGTGCGTTTTCCCGAGTTCGTCCAGTACCTGCTGGACGTGCACCGGCCCGTGGGGATGGACATTCA
CTGGGACCATGTCAGCCGGCTCTGCAGCCCCTGCCTCATCGACTACGATTTCGTAGGCAAGTTCGAGAGC
ATGGAGGACGATGCCAACTTCTTCCTGAGCCTCATCCGCGCGCCGCGGAACCTGACCTTCCCCCGGTTCA
AGGACCGGCACTCGCAGGAGGCGCGGACCACAGCGAGGATCGCCCACCAGTACTTCGCCCAACTCTCGGC
CCTGCAAAGGCAGCGCACCTACGACTTCTACTACATGGATTACCTGATGTTCAACTATTCCAAGCCCTTT
GCAGATCTGTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205818 protein sequence
Red=Cloning site Green=Tags(s)

MTLRPGTMRLACMFSSILLFGAAGLLLFISLQDPTELAPQQVPGIKFNIRPRQPHHDLPPGGSQDGDLKE
PTERVTRDLSSGAPRGRNLPAPDQPQPPLQRGTRLRLRQRRRRLLIKKMPAAATIPANSSDAPFIRPGPG
TLDGRWVSLHRSQQERKRVMQEACAKYRASSSRRAVTPRHVSRIFVEDRHRVLYCEVPKAGCSNWKRVLM
VLAGLASSTADIQHNTVHYGSALKRLDTFDRQGILHRLSTYTKMLFVREPFERLVSAFRDKFEHPNSYYH
PVFGKAILARYRANASREALRTGSGVRFPEFVQYLLDVHRPVGMDIHWDHVSRLCSPCLIDYDFVGKFES
MEDDANFFLSLIRAPRNLTFPRFKDRHSQEARTTARIAHQYFAQLSALQRQRTYDFYYMDYLMFNYSKPF
ADLY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022467
ORF Size 1272 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022467.3, NP_071912.2
RefSeq Size 2496 bp
RefSeq ORF 1275 bp
Locus ID 64377
UniProt ID Q9H2A9
Cytogenetics 19q13.11
Domains Sulfotransfer2
Protein Families Transmembrane
MW 48.8 kDa
Summary The protein encoded by this gene belongs to the sulfotransferase 2 family. It is predominantly expressed in the pituitary gland, and is localized to the golgi membrane. This protein catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. It is responsible for sulfation of GalNAc on luteinizing hormone (LH), which is required for production of the sex hormones. Mice lacking this enzyme, exhibit increased levels of circulating LH, and precocious sexual maturation of both male and female mice. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Aug 2011
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Documents
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"NM_022467" in other vectors (4)
SKU Description Size Price
RC205818L3 Lenti ORF clone of Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 (CHST8), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC205818L4 Lenti ORF clone of Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 (CHST8), transcript variant 3, mGFP tagged 10 ug
$757.00
RG205818 CHST8 (tGFP-tagged) - Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 (CHST8), transcript variant 3 10 ug
$489.00 $657.00
SC319591 CHST8 (untagged)-Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 (CHST8), transcript variant 3 10 ug
$457.00

Plasmid Map for RC205818

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205818
Circular map for RC205818

file-alt Citations

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