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CHST8 (NM_022467) Human Untagged Clone

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SKU
SC319591
CHST8 (untagged)-Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 (CHST8), transcript variant 3
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CHST8
Synonyms GalNAc4ST; GALNAC4ST1; PSS3
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_022467.3 GGGACTCGTCCCAGAGTTTGCTGCCGCCGCCGCCGCTGCCATTAGAGCGGTTTTAATTAG
CTGCAGGATTTTTAATGAGCTGAAATCAAGAAGAGCCCCATCTCAGAAGTAAGCGAGAGC
CGCGCGCAGGGAAGAACTAGTCTGACAAAACAGAAGGAGGTGGTGCTCTCGGGGGACTGG
GCTGTAGGCTCCCACCGATCGATCGGCGACGAGCAGATGAGTTCCCGAGGCCAGGAGGCT
GAGGAGGGAAGCCAGGGCTCAGAGACCAGCGCCCGGTGCAGAGTCCCCGCTGCTGCCACC
GCGGCAGAAGGCACGGTGCCGGATTCAAGGTGACGGCAGTGCCTGGCTCCATTCTCCTCA
AGTTGCCATAGCAGCCTCTCTCCCAACAAGCAGCTTCTCCGGGAGCCCTCAGCCGGGAGG
GACGTCATTGTTCTCTCCTGCCCGTGTGGTGCAAGGAGACCCACCGGACAGAGAACTTTC
CCGAGCCCGGGGTCCGCTGACCACGTGGGTGCACCCATGCTGCCTGGAGCCAGGCACAGG
GACAGGTCCCCACGGCCACAGGGAATCCTCTGGGCAGCTGAGGGGAGCGTCCAGGCCCAG
AAGCAGCTGCAGCCAAGCGTGTCCTTGGAGCCGTCCATGCGTCTGTCCGCCTGCTGCCGG
TCGCCACTGGAGATCTCGGCCTGATGGACGCCTGGTGTGGACGATGAGGGAAGAACGTGC
CCCCCACACCCAAGAGGTGACCCCTGAGCCAGCCCCGGATGACCCTGCGACCTGGAACAA
TGCGGCTGGCCTGCATGTTCTCTTCCATCCTGCTGTTCGGAGCTGCAGGCCTCCTCCTCT
TCATCAGCCTGCAGGACCCTACGGAGCTCGCCCCCCAGCAGGTGCCAGGAATAAAGTTCA
ACATCAGGCCAAGGCAGCCCCACCACGACCTCCCACCAGGCGGCTCCCAGGATGGTGACT
TGAAGGAACCCACAGAGAGGGTCACTCGGGACTTATCCAGTGGGGCCCCGAGGGGCCGCA
ACCTGCCAGCGCCTGACCAGCCTCAACCCCCGCTGCAGAGGGGAACCCGTCTGCGGCTCC
GCCAGCGCCGTCGCCGTCTGCTCATCAAGAAAATGCCAGCTGCGGCGACCATCCCGGCCA
ACAGCTCGGACGCGCCCTTCATCCGGCCGGGACCCGGGACGCTGGATGGCCGCTGGGTCA
GCCTGCACCGGAGCCAGCAGGAGCGCAAGCGGGTGATGCAGGAGGCCTGCGCCAAGTACC
GGGCGAGCAGCAGCCGCCGGGCCGTCACGCCCCGCCACGTGTCCCGTATCTTCGTGGAGG
ACCGCCACCGCGTGCTCTACTGCGAGGTGCCCAAGGCCGGCTGCTCCAATTGGAAGCGGG
TGCTCATGGTGCTGGCCGGCCTGGCCTCGTCCACTGCCGACATCCAGCACAACACCGTCC
ACTATGGCAGCGCTCTCAAGCGCCTGGACACCTTCGACCGCCAGGGTATCTTGCACCGTC
TCAGCACCTACACCAAGATGCTCTTTGTCCGCGAGCCCTTCGAGAGGCTGGTGTCCGCCT
TCCGCGACAAGTTTGAGCACCCCAACAGCTACTATCACCCGGTCTTCGGCAAGGCCATCC
TGGCCCGGTACCGCGCCAATGCCTCTCGGGAGGCCCTGCGGACCGGCTCTGGGGTGCGTT
TTCCCGAGTTCGTCCAGTACCTGCTGGACGTGCACCGGCCCGTGGGGATGGACATTCACT
GGGACCATGTCAGCCGGCTCTGCAGCCCCTGCCTCATCGACTACGATTTCGTAGGCAAGT
TCGAGAGCATGGAGGACGATGCCAACTTCTTCCTGAGCCTCATCCGCGCGCCGCGGAACC
TGACCTTCCCCCGGTTCAAGGACCGGCACTCGCAGGAGGCGCGGACCACAGCGAGGATCG
CCCACCAGTACTTCGCCCAACTCTCGGCCCTGCAAAGGCAGCGCACCTACGACTTCTACT
ACATGGATTACCTGATGTTCAACTATTCCAAGCCCTTTGCAGATCTGTACTGAGGGGCGC
CGCAGCTGGCCGGGGCCGCCCTGCCCCGGTCACTCACCTGTGCTCCCGGGCATCCTCCTG
TCCCTGGCTCCTCATCCTGGGAGCAACAGGGCTCTGAGGACGTGAGGAGCCATCGCTGTG
GGAGGCAGCAGGCCCCGGGTGGGGGGCAGAGGCGCCCAGCCTTGGATGGGGACCCCAGCC
CCTGGCCTGTACCTGTTTCCTCATTCCTTGGCTGAGGGAGAGGCTGAGAACTGGGCAGAC
ACCCCTGGAGCTCAGCCGACAGTTTTGATGAGCAGGGAAGTCTGAGGCCCAGAGGACGGG
GGGCCCAGCGGTAAGGGATGTCCCGCACTCCCTTAGCCATTGCCTTGGACCAAACCACGT
GGTTTGCAGCTTTTCTACGAGCCAGGGGGGAGGTTCCCTTGGATTAAGGTTCCAAATAAA
GCACATGGTTTCCAGAGCAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_022467
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022467.3, NP_071912.2
RefSeq Size 2496 bp
RefSeq ORF 1275 bp
Locus ID 64377
UniProt ID Q9H2A9
Cytogenetics 19q13.11
Domains Sulfotransfer2
Protein Families Transmembrane
Summary The protein encoded by this gene belongs to the sulfotransferase 2 family. It is predominantly expressed in the pituitary gland, and is localized to the golgi membrane. This protein catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. It is responsible for sulfation of GalNAc on luteinizing hormone (LH), which is required for production of the sex hormones. Mice lacking this enzyme, exhibit increased levels of circulating LH, and precocious sexual maturation of both male and female mice. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Aug 2011
Transcript Variant: This variant (3) contains an alternate 5' non-coding exon compared to variant 1. Variants 1-3 encode the same protein.
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Documents
Resources
"NM_022467" in other vectors (4)
SKU Description Size Price
RC205818 CHST8 (Myc-DDK-tagged)-Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 (CHST8), transcript variant 3 10 ug
$457.00
RC205818L3 Lenti ORF clone of Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 (CHST8), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC205818L4 Lenti ORF clone of Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 (CHST8), transcript variant 3, mGFP tagged 10 ug
$757.00
RG205818 CHST8 (tGFP-tagged) - Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 (CHST8), transcript variant 3 10 ug
$489.00 $657.00

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