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TLE6 (NM_024760) Human Tagged ORF Clone

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SKU
RC205815
TLE6 (Myc-DDK-tagged)-Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TLE6
Synonyms GRG6; PREMBL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205815 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACCAGGTCCTCCGACTGGCTCCGGCGGCCTTTGGGGGAGGACAATCAGCCGGAGACCCAGCTGT
TCTGGGACAAGGAGCCTTGGTTTTGGCACGACACTCTGACCGAGCAACTCTGGCGGATTTTTGCCGGCGT
CCACGATGAGAAGGCAAAGCCCAGAGACAGACAGCAGGCACCAGGCCTGGGGCAGGAAAGCAAGGCACCA
GGATCCTGTGACCCAGGAACAGACCCATGTCCTGAAGATGCCTCCACCCCCAGGCCACCTGAGGCCTCCT
CCAGTCCCCCTGAGGGTTCCCAAGACAGGAACACAAGTTGGGGTGTGGTCCAGGAGCCTCCTGGAAGAGC
CTCTCGGTTTCTACAGTCCATATCCTGGGACCCTGAGGACTTTGAAGATGCATGGAAGAGGCCAGATGCC
TTGCCCGGGCAGTCAAAGAGACTCGCCGTCCCGTGCAAACTGGAAAAGATGCGGATCTTGGCACACGGGG
AGCTCGTGCTCGCCACGGCCATCAGCAGCTTCACGCGGCACGTGTTCACCTGTGGCAGAAGAGGCATCAA
GGTGTGGAGCCTGACTGGACAGGTGGCTGAGGACAGGTTCCCTGAGAGCCACCTGCCTATACAGACCCCT
GGGGCCTTCCTGCGCACCTGCCTGCTGTCCTCAAACAGCAGGAGCCTGCTCACCGGTGGCTACAACCTGG
CCAGCGTGAGCGTGTGGGACCTGGCGGCGCCCTCCCTGCATGTGAAGGAGCAGTTGCCCTGTGCAGGTCT
CAACTGCCAGGCCCTGGATGCCAACCTGGATGCCAACCTGGCCTTCGCCAGCTTCACCAGTGGTGTGGTC
AGGATCTGGGACCTGCGGGATCAGAGTGTGGTCAGGGACCTCAAGGGTTATCCTGATGGAGTCAAGAGTA
TCGTGGTCAAGGGCTACAACATCTGGACTGGGGGTCCGGATGCCTGTCTGCGGTGCTGGGACCAGAGGAC
CATCATGAAACCTCTGGAGTACCAATTCAAGTCTCAGATAATGAGCCTGTCCCACAGCCCCCAGGAGGAC
TGGGTGCTGCTGGGCATGGCCAATGGCCAGCAGTGGCTGCAAAGCACCAGCGGGAGCCAGCGGCACATGG
TGGGGCAAAAAGACAGCGTCATCCTGAGCGTCAAGTTCTCCCCCTTTGGCCAGTGGTGGGCAAGCGTTGG
AATGGACGACTTCCTTGGCGTCTACAGCATGCCGGCGGGGACAAAAGTGTTCGAGGTGCCTGAGATGTCT
CCAGTCACGTGCTGTGACGTCTCTTCCAACAACCGCCTCGTTGTCACAGGCTCCGGGGAGCACGCCTCCG
TGTACCAGATCACCTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205815 protein sequence
Red=Cloning site Green=Tags(s)

MATRSSDWLRRPLGEDNQPETQLFWDKEPWFWHDTLTEQLWRIFAGVHDEKAKPRDRQQAPGLGQESKAP
GSCDPGTDPCPEDASTPRPPEASSSPPEGSQDRNTSWGVVQEPPGRASRFLQSISWDPEDFEDAWKRPDA
LPGQSKRLAVPCKLEKMRILAHGELVLATAISSFTRHVFTCGRRGIKVWSLTGQVAEDRFPESHLPIQTP
GAFLRTCLLSSNSRSLLTGGYNLASVSVWDLAAPSLHVKEQLPCAGLNCQALDANLDANLAFASFTSGVV
RIWDLRDQSVVRDLKGYPDGVKSIVVKGYNIWTGGPDACLRCWDQRTIMKPLEYQFKSQIMSLSHSPQED
WVLLGMANGQQWLQSTSGSQRHMVGQKDSVILSVKFSPFGQWWASVGMDDFLGVYSMPAGTKVFEVPEMS
PVTCCDVSSNNRLVVTGSGEHASVYQITY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024760
ORF Size 1347 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024760.3
RefSeq Size 1955 bp
RefSeq ORF 1350 bp
Locus ID 79816
UniProt ID Q9H808, Q6PJM9
Cytogenetics 19p13.3
Protein Families Transcription Factors
MW 49.8 kDa
Summary This gene encodes a member of the Groucho/ transducin-like Enhancer of split family of transcriptional co-repressors. The encoded protein is a component of the mammalian subcortical maternal complex, which is required for preimplantation development. In mouse, knock out of this gene results in cleavage-stage embryonic arrest resulting from defective cytoplasmic F-actin meshwork formation and asymmetric cell division. In human, an allelic variant in this gene is associated with preimplantation embryonic lethality. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2016
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Documents
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"NM_024760" in other vectors (4)
SKU Description Size Price
RC205815L3 Lenti ORF clone of Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC205815L4 Lenti ORF clone of Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 2, mGFP tagged 10 ug
$757.00
RG205815 TLE6 (tGFP-tagged) - Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 2 10 ug
$489.00 $657.00
SC122967 TLE6 (untagged)-Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 2 10 ug
$457.00

Plasmid Map for RC205815

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205815
Circular map for RC205815

file-alt Citations

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