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TLE6 (NM_024760) Human Untagged Clone

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SKU
SC122967
TLE6 (untagged)-Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 2
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TLE6
Synonyms GRG6; PREMBL
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_024760 edited
AACTTGGCTGACCTCCGCAGCTTCCGCCCGACTCTGGCTAAAGTCTTGGAGGCTACTGCC
TTGAAGATGACCTCTAGGGACCAGCCCAGACCCAAGGGCCCCCCGAAAAGCACTTCGCCT
TGTCCTGGGATCTCGAACTCTGAGAGCTCTCCGACGCTGAATTATCAGGGCATTCTAAAT
CGGCTCAAGCAGTTCCCCAGGTTTTCTCCTCATTTTGCTGCGGAGTTGGAGAGCATTTAC
TACTCGCTGCACAAGATCCAGCAGGATGTGGCAGAACATCACAAGCAGATAGGAAACGTC
TTACAGATTGTGGAGAGCTGCAGCCAACTCCAGGGTTTCCAGTCTGAGGAGGTCTCACCT
GCTGAACCAGCCAGCCCTGGGACGCCCCAGCAGGTGAAGGACAAGACCCTGCAGGAGTCG
AGCTTTGAGGACATCATGGCCACCAGGTCCTCCGACTGGCTCCGGCGGCCTTTGGGGGAG
GACAATCAGCCGGAGACCCAGCTGTTCTGGGACAAGGAGCCTTGGTTTTGGCACGACACT
CTGACCGAGCAACTCTGGCGGATTTTTGCCGGCGTCCACGATGAGAAGGCAAAGCCCAGA
GACAGACAGCAGGCACCAGGCCTGGGGCAGGAAAGCAAGGCACCAGGATCCTGTGACCCA
GGAACAGACCCATGTCCTGAAGATGCCTCCACCCCCAGGCCACCTGAGGCCTCCTCCAGT
CCCCCTGAGGGTTCCCAAGACAGGAACACAAGTTGGGGTGTGGTCCAGGAGCCTCCTGGA
AGAGCCTCTCGGTTTCTACAGTCCATATCCTGGGACCCTGAGGACTTTGAAGATGCATGG
AAGAGGCCAGATGCCTTGCCCGGGCAGTCAAAGAGACTCGCCGTCCCGTGCAAACTGGAA
AAGATGCGGATCTTGGCACACGGGGAGCTCGTGCTCGCCACGGCCATCAGCAGCTTCACG
CGGCACGTGTTCACCTGTGGCAGAAGAGGCATCAAGGTGTGGAGCCTGACTGGACAGGTG
GCTGAGGACAGGTTCCCTGAGAGCCACCTGCCTATACAGACCCCTGGGGCCTTCCTGCGC
ACCTGCCTGCTGTCCTCAAACAGCAGGAGCCTGCTCACCGGTGGCTACAACCTGGCCAGC
GTGAGCGTGTGGGACCTGGCGGCGCCCTCCCTGCATGTGAAGGAGCAGTTGCCCTGTGCA
GGTCTCAACTGCCAGGCCCTGGATGCCAACCTGGATGCCAACCTGGCCTTCGCCAGCTTC
ACCAGTGGTGTGGTCAGGATCTGGGACCTGCGGGATCAGAGTGTGGTCAGGGACCTCAAG
GGTTATCCTGATGGAGTCAAGAGTATCGTGGTCAAGGGCTACAACATCTGGACTGGGGGT
CCGGATGCCTGTCTGCGGTGCTGGGACCAGAGGACCATCATGAAACCTCTGGAGTACCAA
TTCAAGTCTCAGATAATGAGCCTGTCCCACAGCCCCCAGGAGGACTGGGTGCTGCTGGGC
ATGGCCAATGGCCAGCAGTGGCTGCAAAGCACCAGCGGGAGCCAGCGGCACATGGTGGGG
CAAAAAGACAGCGTCATCCTGAGCGTCAAGTTCTCCCCCTTTGGCCAGTGGTGGGCAAGC
GTTGGAATGGACGACTTCCTTGGCGTCTACAGCATGCCGGCGGGGACAAAAGTGTTCGAG
GTGCCTGAGATGTCTCCAGTCACGTGCTGTGACGTCTCTTCCAACAACCGCCTCGTTGTC
ACAGGCTCCGGGGAGCACGCCTCCGTGTACCAGATCACCTACTGAGGGGCCTCGCTGCTG
TCATCCCACTCCGGCTCCTCTTTTCATCCCCCCCCTTCCCCCCCCCCAACAAGGGGGACA
TGGTGGAGGGAAGCGGGAAGGCTCTTCTGTGGCATCGCACGATCTAGTCTGTGGTGTAGA
CTGGTCGCCATCACGTGTAATAAAGCACCCGGGAAAGGCAGAGTGAAAAAAAAAAAAAAA
AAAAAAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_024760 unedited
NAGTTCAATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGNAACTTGGC
TGACCTCCGCAGCTTCCGCCCGACTCTGGCTAAAGTCTTGGAGGCTACTGCCTTGAAGAT
GACCTCTAGGGACCAGCCCAGACCCAAGGGCCCCCCGAAAGCACTTCGCCTTGTCCTGGG
ATCTCGAACTCTGAGAGCTCTCCGACGCTGAATTATCAGGGCATTCTAAATCGGCTCAAG
CAGTTCCCCAGGTTTTCTCCTCATTTTGCTGCGGAGTTGGAGAGCATTTACTACTCGCTG
CACAAGATCCAGCAGGATGTGGCAGAACATCACAAGCAGATAGGAAACGTCTTACAGATT
GTGGAGAGCTGCAGCCAACTCCAGGGTTTCCAGTCTGAGGAGGTCTCACCTGCTGAACCA
GCCAGCCCTGGGACGCCCCAGCAGGTGAAGGACAAGACCCTGCAGGAGTCGAGCTTTGAG
GACATCATGGCCACCAGGTCCTCCGACTGGCTCCGGCGGCCTTTGGGGGAGGACAATCAG
CCGGAGACCCAGCTGTTCTGGGACAAGGAGCCTTGGTTTTGGCACGACACTCTGACCGAG
CAACTCTGGCGGATTTTTGCCGGCGTCCACGATGAGAAGGCAAAGCCCAGAGACAGACAG
CAGGCACCAGGCCTGGGGCAGGAAAGCAAGGCACCAGGATCCTGTGACCCAGGAACAGAC
CCATGTCCTGAAGATGCCTCCACCCCCAGGCCACCTGAGGCCTCCTCCAGTCCCNCTGAG
GGTTCCCAAGACAGGAACACAAGTTGGGGGTGTGGTCCAGGAGCCTCCTGGAAGACCTCT
CGGTTTCTACAGTCCATATCCTGGGACCCCTGAGACTTTGAAGATGCATGGNAAGAGCCA
GATGCCTTGA
Restriction Sites Please inquire |
ACCN NM_024760
Insert Size 1990 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024760.1, NP_079036.1
RefSeq Size 1977 bp
RefSeq ORF 1350 bp
Locus ID 79816
UniProt ID Q9H808
Cytogenetics 19p13.3
Protein Families Transcription Factors
Summary This gene encodes a member of the Groucho/ transducin-like Enhancer of split family of transcriptional co-repressors. The encoded protein is a component of the mammalian subcortical maternal complex, which is required for preimplantation development. In mouse, knock out of this gene results in cleavage-stage embryonic arrest resulting from defective cytoplasmic F-actin meshwork formation and asymmetric cell division. In human, an allelic variant in this gene is associated with preimplantation embryonic lethality. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2016
Transcript Variant: This variant (2) lacks an alternate in-frame segment, in the 5' coding region and uses a downstream start codon, compared to variant 1. Isoform 2 has a shorter N-terminus, compared to isoform 1.
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Documents
Resources
"NM_024760" in other vectors (4)
SKU Description Size Price
RC205815 TLE6 (Myc-DDK-tagged)-Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 2 10 ug
$457.00
RC205815L3 Lenti ORF clone of Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC205815L4 Lenti ORF clone of Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 2, mGFP tagged 10 ug
$757.00
RG205815 TLE6 (tGFP-tagged) - Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 2 10 ug
$489.00 $657.00

file-alt Citations

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