CUTC (NM_015960) Human Tagged ORF Clone

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SKU
RC205748
CUTC (Myc-DDK-tagged)-Human cutC copper transporter homolog (E. coli) (CUTC)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CUTC
Synonyms CGI-32
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205748 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAAGGCAGGGGGCCTCCTCTGAGCGAAAACGAGCGCGGATACCGTCCGGGAAGGCCGGAGCAGCAA
ATGGATTTCTCATGGAAGTTTGTGTTGATTCAGTGGAATCAGCTGTGAATGCAGAAAGAGGAGGTGCTGA
TCGGATTGAATTATGTTCTGGTTTATCAGAGGGGGGAACTACACCCAGCATGGGTGTCCTTCAAGTAGTG
AAGCAGAGTGTTCAGATCCCAGTTTTTGTGATGATTCGGCCACGGGGAGGTGATTTTTTGTATTCAGATC
GTGAAATTGAGGTGATGAAGGCTGACATTCGTCTTGCCAAGCTTTATGGTGCTGATGGTTTGGTTTTTGG
GGCATTGACTGAAGATGGACACATTGACAAAGAGCTGTGTATGTCCCTTATGGCTATTTGCCGCCCTCTG
CCAGTCACTTTCCACCGAGCCTTTGACATGGTTCATGATCCAATGGCAGCTCTGGAGACCCTCTTAACCT
TGGGATTTGAACGCGTGTTGACCAGTGGATGTGACAGTTCAGCATTAGAAGGGCTACCCCTAATAAAGCG
ACTCATTGAGCAGGCAAAAGGCAGGATTGTGGTAATGCCAGGAGGTGGTATAACAGACAGAAATCTACAA
AGGATCCTTGAGGGTTCAGGTGCTACAGAATTCCACTGTTCTGCTCGGTCTACTAGAGACTCGGGAATGA
AGTTTCGAAATTCATCTGTTGCCATGGGAGCCTCACTTTCTTGCTCAGAATATTCCCTAAAGGTAACAGA
TGTGACCAAAGTAAGGACTTTGAATGCTATCGCAAAGAACATCCTGGTG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205748 protein sequence
Red=Cloning site Green=Tags(s)

MKRQGASSERKRARIPSGKAGAANGFLMEVCVDSVESAVNAERGGADRIELCSGLSEGGTTPSMGVLQVV
KQSVQIPVFVMIRPRGGDFLYSDREIEVMKADIRLAKLYGADGLVFGALTEDGHIDKELCMSLMAICRPL
PVTFHRAFDMVHDPMAALETLLTLGFERVLTSGCDSSALEGLPLIKRLIEQAKGRIVVMPGGGITDRNLQ
RILEGSGATEFHCSARSTRDSGMKFRNSSVAMGASLSCSEYSLKVTDVTKVRTLNAIAKNILV

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_015960
ORF Size 819 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015960.3
RefSeq Size 1378 bp
RefSeq ORF 822 bp
Locus ID 51076
UniProt ID Q9NTM9
Cytogenetics 10q24.2
MW 29.3 kDa
Summary Members of the CUT family of copper transporters are associated with copper homeostasis and are involved in the uptake, storage, delivery, and efflux of copper (Gupta et al., 1995 PubMed 7635807; Li et al., 2005 PubMed 16182249).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_015960" in other vectors (4)
SKU Description Size Price
RC205748L3 Lenti ORF clone of Human cutC copper transporter homolog (E. coli) (CUTC), Myc-DDK-tagged 10 ug
$600.00
RC205748L4 Lenti ORF clone of Human cutC copper transporter homolog (E. coli) (CUTC), mGFP tagged 10 ug
$600.00
RG205748 CUTC (tGFP-tagged) - Human cutC copper transporter homolog (E. coli) (CUTC) 10 ug
$489.00 $500.00
SC122833 CUTC (untagged)-Human cutC copper transporter homolog (E. coli) (CUTC) 10 ug
$300.00

Plasmid Map for RC205748

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Cloning scheme for RC205748

Circular map for RC205748

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