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PPP2R1B (NM_181699) Human Tagged ORF Clone

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SKU
RC205346
PPP2R1B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $611.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PPP2R1B
Synonyms PP2A-Abeta; PR65B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205346 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCGCATCAGAGCTCGGGACCGGCCCAGGAGCAGCGGGTGGAGATGGAGATGATTCGCTATACC
CGATCGCGGTTTTAATCGACGAGCTCCGCAATGAAGACGTGCAGCTCCGACTCAACAGTATTAAGAAGTT
ATCAACAATTGCCCTAGCACTTGGAGTAGAAAGGACCCGAAGTGAATTGTTGCCATTTCTTACAGATACA
ATTTATGATGAAGATGAGGTACTATTAGCTCTTGCTGAGCAGCTGGGAAATTTCACTGGCCTAGTGGGAG
GTCCTGACTTTGCCCACTGTCTGCTGCCTCCTTTGGAAAATCTGGCAACTGTGGAAGAGACTGTTGTTCG
TGACAAGGCTGTGGAGTCCCTGAGACAGATCTCCCAGGAGCATACTCCTGTTGCTCTGGAAGCTTATTTT
GTACCTCTGGTGAAACGCTTAGCAAGTGGGGATTGGTTCACCTCTCGCACATCTGCATGTGGTTTGTTCA
GCGTTTGCTATCCCAGGGCATCAAATGCTGTTAAAGCAGAAATCAGACAGCAATTCCGTTCCTTGTGCTC
AGATGACACACCAATGGTACGACGTGCTGCTGCTTCCAAATTGGGTGAATTTGCAAAAGTTTTGGAATTA
GACAGTGTGAAAAGTGAAATTGTTCCACTGTTCACTAGTCTAGCTTCAGATGAACAGGATTCAGTGCGCC
TCCTTGCTGTGGAAGCTTGTGTCAGTATTGCCCAGTTATTGTCTCAGGATGACCTTGAGACTTTGGTGAT
GCCTACACTTCGACAAGCAGCAGAAGATAAATCTTGGCGCGTTCGCTATATGGTGGCTGACAGATTTTCA
GAGCTCCAGAAAGCCATGGGTCCTAAAATCACCCTAAATGACCTCATCCCCGCCTTTCAGAACCTACTTA
AAGACTGTGAAGCTGAAGTCCGGGCTGCTGCTGCCCACAAAGTAAAAGAACTTGGTGAGAACTTGCCCAT
TGAAGATAGAGAGACCATAATTATGAATCAAATTCTGCCTTATATAAAGGAATTAGTATCCGATACCAAT
CAACATGTCAAATCGGCTCTAGCTTCTGTAATTATGGGATTGTCTACTATTTTGGGCAAAGAAAATACCA
TTGAACATCTTCTACCTCTTTTCTTAGCTCAGTTAAAGGATGAGTGTCCTGACGTTCGTTTGAATATCAT
CTCCAATTTGGATTGTGTAAATGAAGTGATTGGAATCCGTCAGCTCTCTCAGTCTCTCCTTCCTGCCATA
GTGGAGCTGGCAGAAGATGCCAAATGGAGGGTCCGCCTGGCCATCATTGAGTATATGCCGCTGCTGGCAG
GCCAGCTGGGTGTGGAATTCTTTGATGAAAAGCTGAATTCTTTATGTATGGCTTGGCTCGTGGACCATGT
ATACGCCATCCGAGAAGCTGCCACCAACAACCTCATGAAACTAGTTCAGAAGTTTGGTACAGAGTGGGCC
CAAAATACTATTGTTCCCAAAGTGTTAGTAATGGCAAATGATCCTAATTACTTGCATAGAATGACCACTT
TATTCTGCATTAATGCACTGTCTGAGGCCTGTGGTCAGGAAATAACTACTAAGCAAATGCTGCCCATCGT
ATTAAAAATGGCAGGAGACCAAGTAGCAAATGTTCGCTTCAATGTGGCCAAATCTCTACAAAAGATTGGA
CCAATTCTAGATACCAATGCTTTACAGGGAGAAGTGAAGCCAGTACTACAGAAGTTAGGTCAAGATGAAG
ACATGGATGTCAAATACTTTGCACAGGAAGCTATAAGTGTGGTGGCACAAAGGCTGAGGAAGCTAGAATT
TCCTGTGAAGGACAGTGGAGAACCCAGTGTCCCTCGGGCTGACAAGAACCACTTCCCAAGACCCACAGTG
CCTGGAGAGGACATGGGGAAGGGACCAGTGTATCAGTTGCGTGGAGATACTAGAGACACACTTGCCCAGC
TGGGAATTGCAGAGCTAGTGCATTTCTCCCAAAGCACAGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205346 protein sequence
Red=Cloning site Green=Tags(s)

MAGASELGTGPGAAGGDGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDT
IYDEDEVLLALAEQLGNFTGLVGGPDFAHCLLPPLENLATVEETVVRDKAVESLRQISQEHTPVALEAYF
VPLVKRLASGDWFTSRTSACGLFSVCYPRASNAVKAEIRQQFRSLCSDDTPMVRRAAASKLGEFAKVLEL
DSVKSEIVPLFTSLASDEQDSVRLLAVEACVSIAQLLSQDDLETLVMPTLRQAAEDKSWRVRYMVADRFS
ELQKAMGPKITLNDLIPAFQNLLKDCEAEVRAAAAHKVKELGENLPIEDRETIIMNQILPYIKELVSDTN
QHVKSALASVIMGLSTILGKENTIEHLLPLFLAQLKDECPDVRLNIISNLDCVNEVIGIRQLSQSLLPAI
VELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKFGTEWA
QNTIVPKVLVMANDPNYLHRMTTLFCINALSEACGQEITTKQMLPIVLKMAGDQVANVRFNVAKSLQKIG
PILDTNALQGEVKPVLQKLGQDEDMDVKYFAQEAISVVAQRLRKLEFPVKDSGEPSVPRADKNHFPRPTV
PGEDMGKGPVYQLRGDTRDTLAQLGIAELVHFSQSTD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181699
ORF Size 2001 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181699.3
RefSeq Size 2161 bp
RefSeq ORF 2004 bp
Locus ID 5519
UniProt ID P30154
Cytogenetics 11q23.1
Protein Families Druggable Genome, Phosphatase, Transcription Factors
Protein Pathways Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
MW 73.6 kDa
Summary This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. provided by RefSeq, Apr 2010
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"NM_181699" in other vectors (6)
SKU Description Size Price
RC205346L1 Lenti ORF clone of Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2, Myc-DDK-tagged 10 ug
$911.00
RC205346L2 Lenti ORF clone of Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2, mGFP tagged 10 ug
$911.00
RC205346L3 Lenti ORF clone of Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2, Myc-DDK-tagged 10 ug
$911.00
RC205346L4 Lenti ORF clone of Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2, mGFP tagged 10 ug
$911.00
RG205346 PPP2R1B (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2 10 ug
$789.00 $811.00
SC123371 PPP2R1B (untagged)-Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2 10 ug
$612.00

Plasmid Map for RC205346

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205346
Circular map for RC205346

file-alt Citations

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